Flagellin stimulation suppresses IL-7 secretion of intestinal epithelial cells
IL-7 is a cytokine, which regulates development, maintenance and proliferation of T lymphocytes within the human immune system. Production of IL-7 is observed in a sterile environment such as thymus or bone marrow. However, it is also known that intestinal epithelial cells (IECs) residing in close c...
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Veröffentlicht in: | Cytokine (Philadelphia, Pa.) Pa.), 2008-10, Vol.44 (1), p.57-64 |
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creator | Yoshioka, Atsushi Okamoto, Ryuichi Oshima, Shigeru Akiyama, Junko Tsuchiya, Kiichiro Nakamura, Tetsuya Kanai, Takanori Watanabe, Mamoru |
description | IL-7 is a cytokine, which regulates development, maintenance and proliferation of T lymphocytes within the human immune system. Production of IL-7 is observed in a sterile environment such as thymus or bone marrow. However, it is also known that intestinal epithelial cells (IECs) residing in close contact with numerous bacterial stimuli also produce IL-7. Here we show that secretion of IL-7 by IECs is significantly suppressed upon stimulation by various bacterial components, including flagellin. Analysis of the intracellular mechanism by which flagellin regulates IL-7 production revealed that flagellin down-regulates expression of the two major transcripts encoding IL-7. Surprisingly, such function of flagellin was independent from the known transcriptional regulation of the IL-7 gene, as no significant change was observed in the transcriptional activity regulated by the previously identified promoter region. As the stability of IL-7 mRNA also remained unchanged upon flagellin stimulation, results suggested the possible involvement of a yet unknown transcriptional regulation of the IL-7 gene. These results describe a novel regulation of IL-7 production by bacterial stimuli, presumably mediated via Toll-like receptors. The present system might contribute to regulate the local lymphocyte pool, in response to the gut luminal or sub-mucosal bacterial abundance. |
doi_str_mv | 10.1016/j.cyto.2008.06.004 |
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Production of IL-7 is observed in a sterile environment such as thymus or bone marrow. However, it is also known that intestinal epithelial cells (IECs) residing in close contact with numerous bacterial stimuli also produce IL-7. Here we show that secretion of IL-7 by IECs is significantly suppressed upon stimulation by various bacterial components, including flagellin. Analysis of the intracellular mechanism by which flagellin regulates IL-7 production revealed that flagellin down-regulates expression of the two major transcripts encoding IL-7. Surprisingly, such function of flagellin was independent from the known transcriptional regulation of the IL-7 gene, as no significant change was observed in the transcriptional activity regulated by the previously identified promoter region. As the stability of IL-7 mRNA also remained unchanged upon flagellin stimulation, results suggested the possible involvement of a yet unknown transcriptional regulation of the IL-7 gene. These results describe a novel regulation of IL-7 production by bacterial stimuli, presumably mediated via Toll-like receptors. The present system might contribute to regulate the local lymphocyte pool, in response to the gut luminal or sub-mucosal bacterial abundance.</description><identifier>ISSN: 1043-4666</identifier><identifier>EISSN: 1096-0023</identifier><identifier>DOI: 10.1016/j.cyto.2008.06.004</identifier><identifier>PMID: 18657434</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Cell Line, Tumor ; Colonic Neoplasms ; Down-Regulation ; Flagellin ; Flagellin - pharmacology ; Humans ; IL-7 ; Interleukin-7 - antagonists & inhibitors ; Interleukin-7 - secretion ; Intestinal epithelial cells ; Intestinal Mucosa - cytology ; RNA, Messenger - metabolism</subject><ispartof>Cytokine (Philadelphia, Pa.), 2008-10, Vol.44 (1), p.57-64</ispartof><rights>2008 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c385t-e4cac59cdeabc43867c36bf6541c3c9f8a27656f209992088b02a20ae48800363</citedby><cites>FETCH-LOGICAL-c385t-e4cac59cdeabc43867c36bf6541c3c9f8a27656f209992088b02a20ae48800363</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.cyto.2008.06.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18657434$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yoshioka, Atsushi</creatorcontrib><creatorcontrib>Okamoto, Ryuichi</creatorcontrib><creatorcontrib>Oshima, Shigeru</creatorcontrib><creatorcontrib>Akiyama, Junko</creatorcontrib><creatorcontrib>Tsuchiya, Kiichiro</creatorcontrib><creatorcontrib>Nakamura, Tetsuya</creatorcontrib><creatorcontrib>Kanai, Takanori</creatorcontrib><creatorcontrib>Watanabe, Mamoru</creatorcontrib><title>Flagellin stimulation suppresses IL-7 secretion of intestinal epithelial cells</title><title>Cytokine (Philadelphia, Pa.)</title><addtitle>Cytokine</addtitle><description>IL-7 is a cytokine, which regulates development, maintenance and proliferation of T lymphocytes within the human immune system. Production of IL-7 is observed in a sterile environment such as thymus or bone marrow. However, it is also known that intestinal epithelial cells (IECs) residing in close contact with numerous bacterial stimuli also produce IL-7. Here we show that secretion of IL-7 by IECs is significantly suppressed upon stimulation by various bacterial components, including flagellin. Analysis of the intracellular mechanism by which flagellin regulates IL-7 production revealed that flagellin down-regulates expression of the two major transcripts encoding IL-7. Surprisingly, such function of flagellin was independent from the known transcriptional regulation of the IL-7 gene, as no significant change was observed in the transcriptional activity regulated by the previously identified promoter region. As the stability of IL-7 mRNA also remained unchanged upon flagellin stimulation, results suggested the possible involvement of a yet unknown transcriptional regulation of the IL-7 gene. These results describe a novel regulation of IL-7 production by bacterial stimuli, presumably mediated via Toll-like receptors. The present system might contribute to regulate the local lymphocyte pool, in response to the gut luminal or sub-mucosal bacterial abundance.</description><subject>Cell Line, Tumor</subject><subject>Colonic Neoplasms</subject><subject>Down-Regulation</subject><subject>Flagellin</subject><subject>Flagellin - pharmacology</subject><subject>Humans</subject><subject>IL-7</subject><subject>Interleukin-7 - antagonists & inhibitors</subject><subject>Interleukin-7 - secretion</subject><subject>Intestinal epithelial cells</subject><subject>Intestinal Mucosa - cytology</subject><subject>RNA, Messenger - metabolism</subject><issn>1043-4666</issn><issn>1096-0023</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEFLwzAUx4Mobk6_gAfpyVvra5KmCXiR4VQYetFzyLJXzejamrTCvr2pG3jT0_vD-70_jx8hlzlkOeTiZpPZXd9mFEBmIDIAfkSmOSiRAlB2PGbOUi6EmJCzEDYAoFhZnpJJLkVRcsan5HlRm3esa9ckoXfboTa9a2Meus5jCBiSp2VaJgGtx59NWyWu6THCjakT7Fz_gbWL0caWcE5OKlMHvDjMGXlb3L_OH9Ply8PT_G6ZWiaLPkVujS2UXaNZWc6kKC0Tq0oUPLfMqkoaWopCVBSUUhSkXAE1FAxyKQGYYDNyve_tfPs5xG_01oXxA9NgOwQtlCiZZOpfkOZRG6NFBOketL4NwWOlO--2xu90DnrUrTd61K1H3RqEHu9m5OrQPqy2uP49OfiNwO0ewCjjy6HXwTpsLK6dR9vrdev-6v8GggeRMw</recordid><startdate>20081001</startdate><enddate>20081001</enddate><creator>Yoshioka, Atsushi</creator><creator>Okamoto, Ryuichi</creator><creator>Oshima, Shigeru</creator><creator>Akiyama, Junko</creator><creator>Tsuchiya, Kiichiro</creator><creator>Nakamura, Tetsuya</creator><creator>Kanai, Takanori</creator><creator>Watanabe, Mamoru</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20081001</creationdate><title>Flagellin stimulation suppresses IL-7 secretion of intestinal epithelial cells</title><author>Yoshioka, Atsushi ; Okamoto, Ryuichi ; Oshima, Shigeru ; Akiyama, Junko ; Tsuchiya, Kiichiro ; Nakamura, Tetsuya ; Kanai, Takanori ; Watanabe, Mamoru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c385t-e4cac59cdeabc43867c36bf6541c3c9f8a27656f209992088b02a20ae48800363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Cell Line, Tumor</topic><topic>Colonic Neoplasms</topic><topic>Down-Regulation</topic><topic>Flagellin</topic><topic>Flagellin - pharmacology</topic><topic>Humans</topic><topic>IL-7</topic><topic>Interleukin-7 - antagonists & inhibitors</topic><topic>Interleukin-7 - secretion</topic><topic>Intestinal epithelial cells</topic><topic>Intestinal Mucosa - cytology</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yoshioka, Atsushi</creatorcontrib><creatorcontrib>Okamoto, Ryuichi</creatorcontrib><creatorcontrib>Oshima, Shigeru</creatorcontrib><creatorcontrib>Akiyama, Junko</creatorcontrib><creatorcontrib>Tsuchiya, Kiichiro</creatorcontrib><creatorcontrib>Nakamura, Tetsuya</creatorcontrib><creatorcontrib>Kanai, Takanori</creatorcontrib><creatorcontrib>Watanabe, Mamoru</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cytokine (Philadelphia, Pa.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yoshioka, Atsushi</au><au>Okamoto, Ryuichi</au><au>Oshima, Shigeru</au><au>Akiyama, Junko</au><au>Tsuchiya, Kiichiro</au><au>Nakamura, Tetsuya</au><au>Kanai, Takanori</au><au>Watanabe, Mamoru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Flagellin stimulation suppresses IL-7 secretion of intestinal epithelial cells</atitle><jtitle>Cytokine (Philadelphia, Pa.)</jtitle><addtitle>Cytokine</addtitle><date>2008-10-01</date><risdate>2008</risdate><volume>44</volume><issue>1</issue><spage>57</spage><epage>64</epage><pages>57-64</pages><issn>1043-4666</issn><eissn>1096-0023</eissn><abstract>IL-7 is a cytokine, which regulates development, maintenance and proliferation of T lymphocytes within the human immune system. Production of IL-7 is observed in a sterile environment such as thymus or bone marrow. However, it is also known that intestinal epithelial cells (IECs) residing in close contact with numerous bacterial stimuli also produce IL-7. Here we show that secretion of IL-7 by IECs is significantly suppressed upon stimulation by various bacterial components, including flagellin. Analysis of the intracellular mechanism by which flagellin regulates IL-7 production revealed that flagellin down-regulates expression of the two major transcripts encoding IL-7. Surprisingly, such function of flagellin was independent from the known transcriptional regulation of the IL-7 gene, as no significant change was observed in the transcriptional activity regulated by the previously identified promoter region. As the stability of IL-7 mRNA also remained unchanged upon flagellin stimulation, results suggested the possible involvement of a yet unknown transcriptional regulation of the IL-7 gene. These results describe a novel regulation of IL-7 production by bacterial stimuli, presumably mediated via Toll-like receptors. The present system might contribute to regulate the local lymphocyte pool, in response to the gut luminal or sub-mucosal bacterial abundance.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>18657434</pmid><doi>10.1016/j.cyto.2008.06.004</doi><tpages>8</tpages></addata></record> |
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subjects | Cell Line, Tumor Colonic Neoplasms Down-Regulation Flagellin Flagellin - pharmacology Humans IL-7 Interleukin-7 - antagonists & inhibitors Interleukin-7 - secretion Intestinal epithelial cells Intestinal Mucosa - cytology RNA, Messenger - metabolism |
title | Flagellin stimulation suppresses IL-7 secretion of intestinal epithelial cells |
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