Transformation of a type 4 encapsulated strain of Streptococcus pneumoniae
Abstract Streptococcus pneumoniae strain JNR.7/87 is a highly virulent, type 4 encapsulated Gram-positive bacterium whose transformability has not been tested previously, and whose genome is currently being sequenced. The strain was transformed at very low efficiency by addition of exogenous compete...
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| Veröffentlicht in: | FEMS microbiology letters 1999-03, Vol.172 (2), p.131-135 |
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| creator | Bricker, Angela L. Camilli, Andrew |
| description | Abstract
Streptococcus pneumoniae strain JNR.7/87 is a highly virulent, type 4 encapsulated Gram-positive bacterium whose transformability has not been tested previously, and whose genome is currently being sequenced. The strain was transformed at very low efficiency by addition of exogenous competence-stimulating peptide: However, the efficiency was too low and irreproducible to be useful in many genetic studies. Therefore, the effects on transformation efficiency of changing different components of competence-stimulating peptide-induced transformation have been examined. Screening of growth media was followed by optimization of pre-induction culture acidification, glycine concentration, and induction time. An optimized protocol was developed whereby S. pneumoniae strain JNR.7/87 was transformed reproducibly with a streptomycin resistance (SmR) marker at an efficiency of ~105 colony forming units per 108 cells. |
| doi_str_mv | 10.1111/j.1574-6968.1999.tb13460.x |
| format | Article |
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Streptococcus pneumoniae strain JNR.7/87 is a highly virulent, type 4 encapsulated Gram-positive bacterium whose transformability has not been tested previously, and whose genome is currently being sequenced. The strain was transformed at very low efficiency by addition of exogenous competence-stimulating peptide: However, the efficiency was too low and irreproducible to be useful in many genetic studies. Therefore, the effects on transformation efficiency of changing different components of competence-stimulating peptide-induced transformation have been examined. Screening of growth media was followed by optimization of pre-induction culture acidification, glycine concentration, and induction time. An optimized protocol was developed whereby S. pneumoniae strain JNR.7/87 was transformed reproducibly with a streptomycin resistance (SmR) marker at an efficiency of ~105 colony forming units per 108 cells.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.1999.tb13460.x</identifier><identifier>PMID: 10188240</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Acidification ; Bacterial Proteins - pharmacology ; Biological and medical sciences ; Biotechnology ; Cell culture ; Competence‐stimulating peptide ; Culture media ; Culture Media - chemistry ; DNA-Binding Proteins - pharmacology ; Efficiency ; Encapsulation ; Fundamental and applied biological sciences. Psychology ; Gene transfer ; Genetic engineering ; Genetic technics ; Genetic transformation ; Genomes ; Glycine ; Growth media ; Humans ; Methods. Procedures. Technologies ; Microbiology ; Optimization ; Peptides ; Streptococcus infections ; Streptococcus pneumoniae ; Streptococcus pneumoniae - genetics ; Streptococcus pneumoniae - growth & development ; Streptomycin ; Synthetic digonucleotides and genes. Sequencing ; Time Factors ; Transformation efficiency ; Transformation, Bacterial - drug effects</subject><ispartof>FEMS microbiology letters, 1999-03, Vol.172 (2), p.131-135</ispartof><rights>1999 Federation of European Microbiological Societies. Published by Elsevier Science B. V. All rights reserved. 1999</rights><rights>1999 INIST-CNRS</rights><rights>1999 Federation of European Microbiological Societies. Published by Elsevier Science B. V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3511-ef8fbec06f2d8a867960d7316d86cd9a9103d579302c742ed47e37aefce7f1543</citedby><cites>FETCH-LOGICAL-c3511-ef8fbec06f2d8a867960d7316d86cd9a9103d579302c742ed47e37aefce7f1543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.1999.tb13460.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.1999.tb13460.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1763262$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10188240$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bricker, Angela L.</creatorcontrib><creatorcontrib>Camilli, Andrew</creatorcontrib><title>Transformation of a type 4 encapsulated strain of Streptococcus pneumoniae</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
Streptococcus pneumoniae strain JNR.7/87 is a highly virulent, type 4 encapsulated Gram-positive bacterium whose transformability has not been tested previously, and whose genome is currently being sequenced. The strain was transformed at very low efficiency by addition of exogenous competence-stimulating peptide: However, the efficiency was too low and irreproducible to be useful in many genetic studies. Therefore, the effects on transformation efficiency of changing different components of competence-stimulating peptide-induced transformation have been examined. Screening of growth media was followed by optimization of pre-induction culture acidification, glycine concentration, and induction time. An optimized protocol was developed whereby S. pneumoniae strain JNR.7/87 was transformed reproducibly with a streptomycin resistance (SmR) marker at an efficiency of ~105 colony forming units per 108 cells.</description><subject>Acidification</subject><subject>Bacterial Proteins - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cell culture</subject><subject>Competence‐stimulating peptide</subject><subject>Culture media</subject><subject>Culture Media - chemistry</subject><subject>DNA-Binding Proteins - pharmacology</subject><subject>Efficiency</subject><subject>Encapsulation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene transfer</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Genetic transformation</subject><subject>Genomes</subject><subject>Glycine</subject><subject>Growth media</subject><subject>Humans</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbiology</subject><subject>Optimization</subject><subject>Peptides</subject><subject>Streptococcus infections</subject><subject>Streptococcus pneumoniae</subject><subject>Streptococcus pneumoniae - genetics</subject><subject>Streptococcus pneumoniae - growth & development</subject><subject>Streptomycin</subject><subject>Synthetic digonucleotides and genes. Sequencing</subject><subject>Time Factors</subject><subject>Transformation efficiency</subject><subject>Transformation, Bacterial - drug effects</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqVkUFv1DAQhS1ERZfCX0ARoN6SeuzEdjhUQlULVIs4UM6W1xlLWSVxsBPR_fc4ZAUIgVB98WG-NzNvHiEvgRaQ3sW-gEqWuaiFKqCu62LaAS8FLe4fkc3P0mOyoVyqHGgtT8nTGPeU0pJR8YScAgWlWEk35PYumCE6H3oztX7IvMtMNh1GzMoMB2vGOHdmwiaLUzDtj_rnKeA4eeutnWM2Djj3fmgNPiMnznQRnx__M_Ll5vru6n2-_fTuw9XbbW55BZCjU26HlgrHGmWUkLWgjeQgGiVsU5saKG8qWXPKrCwZNqVELg06i9JBVfIzcr72HYP_OmOcdN9Gi11nBvRz1Mm8BMXof0GQ6XqgIIGv_gD3fg5DMqEZB5BVJegy981K2eBjDOj0GNrehIMGqpdg9F4v118WUHoJRh-D0fdJ_OI4Yt712PwmXZNIwOsjYKI1nUux2Db-4qTgTLCEXa7Yt7bDwwM20Dcft8AXr9XawM_jP-T53wx8B_SZubI</recordid><startdate>199903</startdate><enddate>199903</enddate><creator>Bricker, Angela L.</creator><creator>Camilli, Andrew</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199903</creationdate><title>Transformation of a type 4 encapsulated strain of Streptococcus pneumoniae</title><author>Bricker, Angela L. ; Camilli, Andrew</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3511-ef8fbec06f2d8a867960d7316d86cd9a9103d579302c742ed47e37aefce7f1543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Acidification</topic><topic>Bacterial Proteins - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cell culture</topic><topic>Competence‐stimulating peptide</topic><topic>Culture media</topic><topic>Culture Media - chemistry</topic><topic>DNA-Binding Proteins - pharmacology</topic><topic>Efficiency</topic><topic>Encapsulation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene transfer</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Genetic transformation</topic><topic>Genomes</topic><topic>Glycine</topic><topic>Growth media</topic><topic>Humans</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbiology</topic><topic>Optimization</topic><topic>Peptides</topic><topic>Streptococcus infections</topic><topic>Streptococcus pneumoniae</topic><topic>Streptococcus pneumoniae - genetics</topic><topic>Streptococcus pneumoniae - growth & development</topic><topic>Streptomycin</topic><topic>Synthetic digonucleotides and genes. Sequencing</topic><topic>Time Factors</topic><topic>Transformation efficiency</topic><topic>Transformation, Bacterial - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bricker, Angela L.</creatorcontrib><creatorcontrib>Camilli, Andrew</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bricker, Angela L.</au><au>Camilli, Andrew</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transformation of a type 4 encapsulated strain of Streptococcus pneumoniae</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>1999-03</date><risdate>1999</risdate><volume>172</volume><issue>2</issue><spage>131</spage><epage>135</epage><pages>131-135</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Abstract
Streptococcus pneumoniae strain JNR.7/87 is a highly virulent, type 4 encapsulated Gram-positive bacterium whose transformability has not been tested previously, and whose genome is currently being sequenced. The strain was transformed at very low efficiency by addition of exogenous competence-stimulating peptide: However, the efficiency was too low and irreproducible to be useful in many genetic studies. Therefore, the effects on transformation efficiency of changing different components of competence-stimulating peptide-induced transformation have been examined. Screening of growth media was followed by optimization of pre-induction culture acidification, glycine concentration, and induction time. An optimized protocol was developed whereby S. pneumoniae strain JNR.7/87 was transformed reproducibly with a streptomycin resistance (SmR) marker at an efficiency of ~105 colony forming units per 108 cells.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>10188240</pmid><doi>10.1111/j.1574-6968.1999.tb13460.x</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1999-03, Vol.172 (2), p.131-135 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69671820 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Acidification Bacterial Proteins - pharmacology Biological and medical sciences Biotechnology Cell culture Competence‐stimulating peptide Culture media Culture Media - chemistry DNA-Binding Proteins - pharmacology Efficiency Encapsulation Fundamental and applied biological sciences. Psychology Gene transfer Genetic engineering Genetic technics Genetic transformation Genomes Glycine Growth media Humans Methods. Procedures. Technologies Microbiology Optimization Peptides Streptococcus infections Streptococcus pneumoniae Streptococcus pneumoniae - genetics Streptococcus pneumoniae - growth & development Streptomycin Synthetic digonucleotides and genes. Sequencing Time Factors Transformation efficiency Transformation, Bacterial - drug effects |
| title | Transformation of a type 4 encapsulated strain of Streptococcus pneumoniae |
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