Effects of a purified fraction from Echeveria gibbiflora aqueous crude extract on guinea-pig spermatozoa
Guinea‐pig spermatozoa in the presence of a purified fraction from Echeveria gibbiflora aqueous crude extract suffer a hypotonic‐like effect. The phenomena exhibited included a distension of the plasma membrane over the acrosome region, inducing the formation of a huge ‘head‐bubble’. The agglutinati...
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| Veröffentlicht in: | Phytotherapy research 1999-02, Vol.13 (1), p.46-49 |
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| creator | Delgado, Néstor M. Ramírez, Javier Taboada Hernández, Alfredo Ortega Merchant-Larios, Horacio Sánchez-Vázqueź, María Luisa Ramírez, Guillermo Reyes, Rosalina |
| description | Guinea‐pig spermatozoa in the presence of a purified fraction from Echeveria gibbiflora aqueous crude extract suffer a hypotonic‐like effect. The phenomena exhibited included a distension of the plasma membrane over the acrosome region, inducing the formation of a huge ‘head‐bubble’. The agglutination effect was so enhanced that instead of inducing sperm clusters, it produced cane‐like ‘stalk’ structures. The immobilizing activity was induced instantaneously after the addition of the purified fraction. At electron microscope level it was possible to observe a heavy amount of electron dense material of the purified fraction embedded or intercalated along the plasma membrane. It was also possible to corroborate the dispersion of the acrosomal content and the disappearance of the external acrosome membrane. The purified fraction induced loosening of the plasma membrane all along the sperm cell, however, the distension of the membrane was only produced in the apical portion of the sperm head and not in the post equatorial region. The results suggest that the plant may yield a compound suitable for use as a vaginal barrier or male contraceptive agent. Copyright © 1999 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/(SICI)1099-1573(199902)13:1<46::AID-PTR373>3.0.CO;2-S |
| format | Article |
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The phenomena exhibited included a distension of the plasma membrane over the acrosome region, inducing the formation of a huge ‘head‐bubble’. The agglutination effect was so enhanced that instead of inducing sperm clusters, it produced cane‐like ‘stalk’ structures. The immobilizing activity was induced instantaneously after the addition of the purified fraction. At electron microscope level it was possible to observe a heavy amount of electron dense material of the purified fraction embedded or intercalated along the plasma membrane. It was also possible to corroborate the dispersion of the acrosomal content and the disappearance of the external acrosome membrane. The purified fraction induced loosening of the plasma membrane all along the sperm cell, however, the distension of the membrane was only produced in the apical portion of the sperm head and not in the post equatorial region. The results suggest that the plant may yield a compound suitable for use as a vaginal barrier or male contraceptive agent. Copyright © 1999 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0951-418X</identifier><identifier>EISSN: 1099-1573</identifier><identifier>DOI: 10.1002/(SICI)1099-1573(199902)13:1<46::AID-PTR373>3.0.CO;2-S</identifier><identifier>PMID: 10189950</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>acrosome ; Acrosome - drug effects ; Acrosome - ultrastructure ; agglutination ; Animals ; Biological and medical sciences ; Biological Assay ; General pharmacology ; Guinea Pigs ; hyperosmotic ; immobilization ; In Vitro Techniques ; Male ; Medical sciences ; Mexico ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Plant Extracts - pharmacology ; Plants, Medicinal - chemistry ; Sperm Motility - drug effects ; Spermatozoa - drug effects ; Spermatozoa - ultrastructure</subject><ispartof>Phytotherapy research, 1999-02, Vol.13 (1), p.46-49</ispartof><rights>Copyright © 1999 John Wiley & Sons, Ltd.</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F%28SICI%291099-1573%28199902%2913%3A1%3C46%3A%3AAID-PTR373%3E3.0.CO%3B2-S$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F%28SICI%291099-1573%28199902%2913%3A1%3C46%3A%3AAID-PTR373%3E3.0.CO%3B2-S$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1733050$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10189950$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Delgado, Néstor M.</creatorcontrib><creatorcontrib>Ramírez, Javier Taboada</creatorcontrib><creatorcontrib>Hernández, Alfredo Ortega</creatorcontrib><creatorcontrib>Merchant-Larios, Horacio</creatorcontrib><creatorcontrib>Sánchez-Vázqueź, María Luisa</creatorcontrib><creatorcontrib>Ramírez, Guillermo</creatorcontrib><creatorcontrib>Reyes, Rosalina</creatorcontrib><title>Effects of a purified fraction from Echeveria gibbiflora aqueous crude extract on guinea-pig spermatozoa</title><title>Phytotherapy research</title><addtitle>Phytother. Res</addtitle><description>Guinea‐pig spermatozoa in the presence of a purified fraction from Echeveria gibbiflora aqueous crude extract suffer a hypotonic‐like effect. The phenomena exhibited included a distension of the plasma membrane over the acrosome region, inducing the formation of a huge ‘head‐bubble’. The agglutination effect was so enhanced that instead of inducing sperm clusters, it produced cane‐like ‘stalk’ structures. The immobilizing activity was induced instantaneously after the addition of the purified fraction. At electron microscope level it was possible to observe a heavy amount of electron dense material of the purified fraction embedded or intercalated along the plasma membrane. It was also possible to corroborate the dispersion of the acrosomal content and the disappearance of the external acrosome membrane. The purified fraction induced loosening of the plasma membrane all along the sperm cell, however, the distension of the membrane was only produced in the apical portion of the sperm head and not in the post equatorial region. The results suggest that the plant may yield a compound suitable for use as a vaginal barrier or male contraceptive agent. Copyright © 1999 John Wiley & Sons, Ltd.</description><subject>acrosome</subject><subject>Acrosome - drug effects</subject><subject>Acrosome - ultrastructure</subject><subject>agglutination</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biological Assay</subject><subject>General pharmacology</subject><subject>Guinea Pigs</subject><subject>hyperosmotic</subject><subject>immobilization</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mexico</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Plant Extracts - pharmacology</subject><subject>Plants, Medicinal - chemistry</subject><subject>Sperm Motility - drug effects</subject><subject>Spermatozoa - drug effects</subject><subject>Spermatozoa - ultrastructure</subject><issn>0951-418X</issn><issn>1099-1573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkdFu0zAUhi0EYt3gFZAvENouUuw4seMOJo1QRqWJoq2oiJsjJ3Y6Q9IUO4GNp8chZXBly_r-X8fnQ-g1JVNKSPzy-HqRL04okTKiqWDHVEpJ4hPKZvRVwmez88Xb6OPqigl2xqZkmi9P4-j6AZrcJx6iCZEpjRKafT5Ah95_JYTImCSP0QElNJMyJRN0M68qU3YetxVWeNc7W1mjceVU2dl2Gy5tg-fljflhnFV4Y4vCVnXrFFbfe9P2Hpeu1wab226I4BDZ9HZrVLSzG-x3xjWqa3-16gl6VKnam6f78wh9ejdf5e-jy-XFIj-_jGzMJYvC4DpjjNMsS4nWsjCxZkUpeVroUoTPUUUYiwXPjBA0jhNpqK604IQLwjLFjtCLsXfn2jCh76CxvjR1rbbDuMAl55lMZACf7cG-aIyGnbONcnfwdzcBeL4HlC9VHVayLa3_xwnGyB9sPWI_bW3u_quBwSMMGmGQAoMUGDUCDTdIOASLMFoEBgTyJYTE_iU0R2Oz9Z25vW9W7htwwUQK6w8XkK_ffOHZ6go4-w2oUqRA</recordid><startdate>199902</startdate><enddate>199902</enddate><creator>Delgado, Néstor M.</creator><creator>Ramírez, Javier Taboada</creator><creator>Hernández, Alfredo Ortega</creator><creator>Merchant-Larios, Horacio</creator><creator>Sánchez-Vázqueź, María Luisa</creator><creator>Ramírez, Guillermo</creator><creator>Reyes, Rosalina</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>199902</creationdate><title>Effects of a purified fraction from Echeveria gibbiflora aqueous crude extract on guinea-pig spermatozoa</title><author>Delgado, Néstor M. ; Ramírez, Javier Taboada ; Hernández, Alfredo Ortega ; Merchant-Larios, Horacio ; Sánchez-Vázqueź, María Luisa ; Ramírez, Guillermo ; Reyes, Rosalina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i2693-573d833618850dd9be2d3bc965bdc71091a0332768e7712249e1dfd76067038a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>acrosome</topic><topic>Acrosome - drug effects</topic><topic>Acrosome - ultrastructure</topic><topic>agglutination</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biological Assay</topic><topic>General pharmacology</topic><topic>Guinea Pigs</topic><topic>hyperosmotic</topic><topic>immobilization</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mexico</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>Plant Extracts - pharmacology</topic><topic>Plants, Medicinal - chemistry</topic><topic>Sperm Motility - drug effects</topic><topic>Spermatozoa - drug effects</topic><topic>Spermatozoa - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Delgado, Néstor M.</creatorcontrib><creatorcontrib>Ramírez, Javier Taboada</creatorcontrib><creatorcontrib>Hernández, Alfredo Ortega</creatorcontrib><creatorcontrib>Merchant-Larios, Horacio</creatorcontrib><creatorcontrib>Sánchez-Vázqueź, María Luisa</creatorcontrib><creatorcontrib>Ramírez, Guillermo</creatorcontrib><creatorcontrib>Reyes, Rosalina</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Phytotherapy research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Delgado, Néstor M.</au><au>Ramírez, Javier Taboada</au><au>Hernández, Alfredo Ortega</au><au>Merchant-Larios, Horacio</au><au>Sánchez-Vázqueź, María Luisa</au><au>Ramírez, Guillermo</au><au>Reyes, Rosalina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of a purified fraction from Echeveria gibbiflora aqueous crude extract on guinea-pig spermatozoa</atitle><jtitle>Phytotherapy research</jtitle><addtitle>Phytother. Res</addtitle><date>1999-02</date><risdate>1999</risdate><volume>13</volume><issue>1</issue><spage>46</spage><epage>49</epage><pages>46-49</pages><issn>0951-418X</issn><eissn>1099-1573</eissn><abstract>Guinea‐pig spermatozoa in the presence of a purified fraction from Echeveria gibbiflora aqueous crude extract suffer a hypotonic‐like effect. The phenomena exhibited included a distension of the plasma membrane over the acrosome region, inducing the formation of a huge ‘head‐bubble’. The agglutination effect was so enhanced that instead of inducing sperm clusters, it produced cane‐like ‘stalk’ structures. The immobilizing activity was induced instantaneously after the addition of the purified fraction. At electron microscope level it was possible to observe a heavy amount of electron dense material of the purified fraction embedded or intercalated along the plasma membrane. It was also possible to corroborate the dispersion of the acrosomal content and the disappearance of the external acrosome membrane. The purified fraction induced loosening of the plasma membrane all along the sperm cell, however, the distension of the membrane was only produced in the apical portion of the sperm head and not in the post equatorial region. The results suggest that the plant may yield a compound suitable for use as a vaginal barrier or male contraceptive agent. Copyright © 1999 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>10189950</pmid><doi>10.1002/(SICI)1099-1573(199902)13:1<46::AID-PTR373>3.0.CO;2-S</doi><tpages>4</tpages></addata></record> |
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| subjects | acrosome Acrosome - drug effects Acrosome - ultrastructure agglutination Animals Biological and medical sciences Biological Assay General pharmacology Guinea Pigs hyperosmotic immobilization In Vitro Techniques Male Medical sciences Mexico Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Plant Extracts - pharmacology Plants, Medicinal - chemistry Sperm Motility - drug effects Spermatozoa - drug effects Spermatozoa - ultrastructure |
| title | Effects of a purified fraction from Echeveria gibbiflora aqueous crude extract on guinea-pig spermatozoa |
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