Enzymatic properties of vesicle-reconstituted human cytochrome P450SCC (CYP11A1) differences in functioning of the mitochondrial electron-transfer chain using human and bovine adrenodoxin and activation by cardiolipin

The recently reported heterologous expression and purification of both human cytochrome P450SCC and adrenodoxin [Woods, S.T., Sadleir, J., Downs, T., Triantopoulos, T., Haedlam, M.J. & Tuckey, R.C. (1998) Arch. Biochem. Biophys. 353, 109-115] has enabled us to perform studies with the membrane-r...

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Veröffentlicht in:	European journal of biochemistry 1999-03, Vol.260 (3), p.768-773
Hauptverfasser:	Kisselev, P, Tuckey, R C, Woods, S T, Triantopoulos, T, Schwarz, D
Format:	Artikel
Sprache:	eng
Schlagworte:	Adrenodoxin - pharmacology Animals Binding Sites Cardiolipins - pharmacology Cattle Cholesterol - metabolism Cholesterol Side-Chain Cleavage Enzyme - isolation & purification Cholesterol Side-Chain Cleavage Enzyme - metabolism Electron Transport - drug effects Enzyme Activation Humans Kinetics Micelles Mitochondria - drug effects Mitochondria - enzymology Mitochondria - metabolism Oxidation-Reduction Phospholipids - metabolism Polysorbates - pharmacology Species Specificity
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