A novel microtiter plate based method for identification of B-cell epitopes

A new type of microtiter plate capable of binding biomolecules covalently in a one step procedure was used to map linear B‐cell epitopes in two different proteins using a peptide‐based solid phase immunoassay. The method was compared with a conventional immobilization method using passive adsorption...

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Veröffentlicht in:	Journal of peptide science 1999-02, Vol.5 (2), p.75-82
Hauptverfasser:	Gregorius, Klaus, Dalum, Iben, Freisleben, Marianne, Mouritsen, Søren, Elsner, Henrik I.
Format:	Artikel
Sprache:	eng
Schlagworte:	aa, amino acid amino acid Amino Acid Sequence Animals Antibody Specificity AquaBind B-Lymphocytes - immunology bovine serum albumin BSA BSA, bovine serum albumin covalent binding Dextrans - chemistry Dextrans - metabolism Epitope Mapping - methods epitope scan Immunochemistry - instrumentation Immunochemistry - methods Mice microtiter plate Molecular Sequence Data mTNFα mTNFα, murine tumor necrosis factor‐α murine tumor necrosis factor-α N-hydroxy succinimide NHS NHS, N‐hydroxy succinimide peptide ELISA Peptides - immunology Peptides - metabolism PLL PLL, poly l‐lysine poly l-lysine room temperature RT, room temperature Titrimetry - instrumentation Tumor Necrosis Factor-alpha - immunology
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