Semen processing by density gradient centrifugation does not improve sperm apoptotic deoxyribonucleic acid fragmentation rates
We wished to verify whether semen processing by discontinuous double-layered density gradient centrifugation could improve sperm apoptotic DNA fragmentation rates using a commercially available deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling assay in 35 consecutive...
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| Veröffentlicht in: | Fertility and sterility 2008-09, Vol.90 (3), p.889-890 |
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| container_title | Fertility and sterility |
| container_volume | 90 |
| creator | Stevanato, Juliana, M.S Bertolla, Ricardo Pimenta, Ph.D., D.V.M Barradas, Valeria, B.S Spaine, Deborah M., M.S Cedenho, Agnaldo P., M.D., Ph.D Ortiz, Valdemar, M.D., Ph.D |
| description | We wished to verify whether semen processing by discontinuous double-layered density gradient centrifugation could improve sperm apoptotic DNA fragmentation rates using a commercially available deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling assay in 35 consecutive men presenting for assisted reproductive treatments. Although sperm motility did improve as expected, no effects were observed in sperm apoptotic DNA fragmentation rates, and this should be considered in the routine assisted reproduction setting. |
| doi_str_mv | 10.1016/j.fertnstert.2007.01.059 |
| format | Article |
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| ispartof | Fertility and sterility, 2008-09, Vol.90 (3), p.889-890 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Adult Biological and medical sciences Centrifugation, Density Gradient - methods DNA - analysis DNA - genetics DNA Fragmentation Gynecology. Andrology. Obstetrics Humans Internal Medicine Male Medical sciences Obstetrics and Gynecology Specimen Handling - methods Sperm Motility - genetics Spermatozoa - cytology Spermatozoa - physiology |
| title | Semen processing by density gradient centrifugation does not improve sperm apoptotic deoxyribonucleic acid fragmentation rates |
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