The impact of spermatozoa preincubation time and spontaneous acrosome reaction in intracytoplasmic sperm injection: a controlled randomized study
Objective To determine the optimum time interval between semen processing and incubation before intracytoplasmic sperm injection (ICSI) and correlate it with the acrosomal reaction rate. Design Controlled randomized study. Setting The Egyptian IVF-ET Center. Patient(s) Couples with male factor infer...
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| Veröffentlicht in: | Fertility and sterility 2008-09, Vol.90 (3), p.584-591 |
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| creator | Mansour, Ragaa T., M.D., Ph.D Serour, Mennatallah G., B.Sc Abbas, Amal M., M.D Kamal, Ahmed, M.D Tawab, Nevine A., B.Sc Aboulghar, Mohamed A., M.D Serour, Gamal I., M.D., FRCOG |
| description | Objective To determine the optimum time interval between semen processing and incubation before intracytoplasmic sperm injection (ICSI) and correlate it with the acrosomal reaction rate. Design Controlled randomized study. Setting The Egyptian IVF-ET Center. Patient(s) Couples with male factor infertility undergoing ICSI using ejaculated semen. Intervention(s) The patients were prospectively randomized according to differences in sperm preincubation time before ICSI into 1-hour, 3-hour, and 5-hour groups. The status of the acrosome was studied using electron microscopy. Main Outcome Measure(s) The primary outcome measures were fertilization rate and acrosome reaction rate. Secondary outcome measures were the implantation and pregnancy rates. Result(s) The rate of acrosomally reacted spermatozoa was the highest (68.2%) after 5 hours of incubation and lowest (25.6%) after 1 hour of incubation. The difference was statistically significant. The fertilization rate was the highest (74%) using spermatozoa incubated for 3 hours as compared with 1 hour (70%) and 5 hours (67%), but the difference was not statistically significant. Conclusion(s) Acrosome reaction is time dependent; the optimum incubation time of spermatozoa before ICSI was 3 hours, which resulted in the highest fertilization rate. |
| doi_str_mv | 10.1016/j.fertnstert.2006.11.176 |
| format | Article |
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Design Controlled randomized study. Setting The Egyptian IVF-ET Center. Patient(s) Couples with male factor infertility undergoing ICSI using ejaculated semen. Intervention(s) The patients were prospectively randomized according to differences in sperm preincubation time before ICSI into 1-hour, 3-hour, and 5-hour groups. The status of the acrosome was studied using electron microscopy. Main Outcome Measure(s) The primary outcome measures were fertilization rate and acrosome reaction rate. Secondary outcome measures were the implantation and pregnancy rates. Result(s) The rate of acrosomally reacted spermatozoa was the highest (68.2%) after 5 hours of incubation and lowest (25.6%) after 1 hour of incubation. The difference was statistically significant. The fertilization rate was the highest (74%) using spermatozoa incubated for 3 hours as compared with 1 hour (70%) and 5 hours (67%), but the difference was not statistically significant. Conclusion(s) Acrosome reaction is time dependent; the optimum incubation time of spermatozoa before ICSI was 3 hours, which resulted in the highest fertilization rate.</description><identifier>ISSN: 0015-0282</identifier><identifier>EISSN: 1556-5653</identifier><identifier>DOI: 10.1016/j.fertnstert.2006.11.176</identifier><identifier>PMID: 18295761</identifier><identifier>CODEN: FESTAS</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Acrosome Reaction ; Adult ; Biological and medical sciences ; Egypt - epidemiology ; electron microscopy ; Female ; fertilization ; Gynecology. Andrology. Obstetrics ; Humans ; ICSI ; Infertility, Male - epidemiology ; Infertility, Male - therapy ; Internal Medicine ; Male ; Medical sciences ; Obstetrics and Gynecology ; Pregnancy ; Pregnancy Rate ; Semen Preservation - methods ; Semen Preservation - statistics & numerical data ; Sperm Injections, Intracytoplasmic - statistics & numerical data ; sperm preincubation time ; Time Factors ; Treatment Outcome</subject><ispartof>Fertility and sterility, 2008-09, Vol.90 (3), p.584-591</ispartof><rights>American Society for Reproductive Medicine</rights><rights>2008 American Society for Reproductive Medicine</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c507t-da63654519d8853cb9f36bc0a578936cf4b2f6185e6b1e4cc4fad95b8ecbd6673</citedby><cites>FETCH-LOGICAL-c507t-da63654519d8853cb9f36bc0a578936cf4b2f6185e6b1e4cc4fad95b8ecbd6673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.fertnstert.2006.11.176$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20656528$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18295761$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mansour, Ragaa T., M.D., Ph.D</creatorcontrib><creatorcontrib>Serour, Mennatallah G., B.Sc</creatorcontrib><creatorcontrib>Abbas, Amal M., M.D</creatorcontrib><creatorcontrib>Kamal, Ahmed, M.D</creatorcontrib><creatorcontrib>Tawab, Nevine A., B.Sc</creatorcontrib><creatorcontrib>Aboulghar, Mohamed A., M.D</creatorcontrib><creatorcontrib>Serour, Gamal I., M.D., FRCOG</creatorcontrib><title>The impact of spermatozoa preincubation time and spontaneous acrosome reaction in intracytoplasmic sperm injection: a controlled randomized study</title><title>Fertility and sterility</title><addtitle>Fertil Steril</addtitle><description>Objective To determine the optimum time interval between semen processing and incubation before intracytoplasmic sperm injection (ICSI) and correlate it with the acrosomal reaction rate. Design Controlled randomized study. Setting The Egyptian IVF-ET Center. Patient(s) Couples with male factor infertility undergoing ICSI using ejaculated semen. Intervention(s) The patients were prospectively randomized according to differences in sperm preincubation time before ICSI into 1-hour, 3-hour, and 5-hour groups. The status of the acrosome was studied using electron microscopy. Main Outcome Measure(s) The primary outcome measures were fertilization rate and acrosome reaction rate. Secondary outcome measures were the implantation and pregnancy rates. Result(s) The rate of acrosomally reacted spermatozoa was the highest (68.2%) after 5 hours of incubation and lowest (25.6%) after 1 hour of incubation. The difference was statistically significant. The fertilization rate was the highest (74%) using spermatozoa incubated for 3 hours as compared with 1 hour (70%) and 5 hours (67%), but the difference was not statistically significant. Conclusion(s) Acrosome reaction is time dependent; the optimum incubation time of spermatozoa before ICSI was 3 hours, which resulted in the highest fertilization rate.</description><subject>Acrosome Reaction</subject><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Egypt - epidemiology</subject><subject>electron microscopy</subject><subject>Female</subject><subject>fertilization</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>ICSI</subject><subject>Infertility, Male - epidemiology</subject><subject>Infertility, Male - therapy</subject><subject>Internal Medicine</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Obstetrics and Gynecology</subject><subject>Pregnancy</subject><subject>Pregnancy Rate</subject><subject>Semen Preservation - methods</subject><subject>Semen Preservation - statistics & numerical data</subject><subject>Sperm Injections, Intracytoplasmic - statistics & numerical data</subject><subject>sperm preincubation time</subject><subject>Time Factors</subject><subject>Treatment Outcome</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNks-O1SAUxonRONfRVzBsdNcKtNDWhYlO_JdM4sJxTSicRq5tqUAnufMWvrGn9sZJXJkQIPA7H_B9EEI5Kznj6tWxHCDmOWXsS8GYKjkveaMekAOXUhVSyeohOTDGZcFEKy7Ik5SODEHeiMfkgreik43iB_Lr5jtQPy3GZhoGmhaIk8nhLhi6RPCzXXuTfZhp9hNQMztEwpzNDGFN1NgYUsCNCCiwYX5rORp7ymEZTZq83UVx-Qh_mNfUUIsaMYwjOBpRNEz-Dqcpr-70lDwazJjg2Xm8JN8-vL-5-lRcf_n4-ertdWEla3LhjKqUrCXvXNvKyvbdUKneMiObtquUHepeDIq3ElTPoba2HozrZN-C7Z1STXVJXu66Sww_V0hZTz5ZGMf9bVp1SglsCLY7uD02RRj0Ev1k4klzprc49FHfx6G3ODTnGuPA0ufnM9Z-AndfePYfgRdnwCRrxgHNsD795QRTGKVokXu3c4CO3HqIOlkPswXnI7qqXfD_c5s3_4jY0c8ez_0BJ0jHsMYZHddcJ6GZ_rp9n-33MMXqpqpZ9RvFLMhB</recordid><startdate>20080901</startdate><enddate>20080901</enddate><creator>Mansour, Ragaa T., M.D., Ph.D</creator><creator>Serour, Mennatallah G., B.Sc</creator><creator>Abbas, Amal M., M.D</creator><creator>Kamal, Ahmed, M.D</creator><creator>Tawab, Nevine A., B.Sc</creator><creator>Aboulghar, Mohamed A., M.D</creator><creator>Serour, Gamal I., M.D., FRCOG</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080901</creationdate><title>The impact of spermatozoa preincubation time and spontaneous acrosome reaction in intracytoplasmic sperm injection: a controlled randomized study</title><author>Mansour, Ragaa T., M.D., Ph.D ; Serour, Mennatallah G., B.Sc ; Abbas, Amal M., M.D ; Kamal, Ahmed, M.D ; Tawab, Nevine A., B.Sc ; Aboulghar, Mohamed A., M.D ; Serour, Gamal I., M.D., FRCOG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c507t-da63654519d8853cb9f36bc0a578936cf4b2f6185e6b1e4cc4fad95b8ecbd6673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Acrosome Reaction</topic><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Egypt - epidemiology</topic><topic>electron microscopy</topic><topic>Female</topic><topic>fertilization</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>ICSI</topic><topic>Infertility, Male - epidemiology</topic><topic>Infertility, Male - therapy</topic><topic>Internal Medicine</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Obstetrics and Gynecology</topic><topic>Pregnancy</topic><topic>Pregnancy Rate</topic><topic>Semen Preservation - methods</topic><topic>Semen Preservation - statistics & numerical data</topic><topic>Sperm Injections, Intracytoplasmic - statistics & numerical data</topic><topic>sperm preincubation time</topic><topic>Time Factors</topic><topic>Treatment Outcome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mansour, Ragaa T., M.D., Ph.D</creatorcontrib><creatorcontrib>Serour, Mennatallah G., B.Sc</creatorcontrib><creatorcontrib>Abbas, Amal M., M.D</creatorcontrib><creatorcontrib>Kamal, Ahmed, M.D</creatorcontrib><creatorcontrib>Tawab, Nevine A., B.Sc</creatorcontrib><creatorcontrib>Aboulghar, Mohamed A., M.D</creatorcontrib><creatorcontrib>Serour, Gamal I., M.D., FRCOG</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Fertility and sterility</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mansour, Ragaa T., M.D., Ph.D</au><au>Serour, Mennatallah G., B.Sc</au><au>Abbas, Amal M., M.D</au><au>Kamal, Ahmed, M.D</au><au>Tawab, Nevine A., B.Sc</au><au>Aboulghar, Mohamed A., M.D</au><au>Serour, Gamal I., M.D., FRCOG</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The impact of spermatozoa preincubation time and spontaneous acrosome reaction in intracytoplasmic sperm injection: a controlled randomized study</atitle><jtitle>Fertility and sterility</jtitle><addtitle>Fertil Steril</addtitle><date>2008-09-01</date><risdate>2008</risdate><volume>90</volume><issue>3</issue><spage>584</spage><epage>591</epage><pages>584-591</pages><issn>0015-0282</issn><eissn>1556-5653</eissn><coden>FESTAS</coden><abstract>Objective To determine the optimum time interval between semen processing and incubation before intracytoplasmic sperm injection (ICSI) and correlate it with the acrosomal reaction rate. Design Controlled randomized study. Setting The Egyptian IVF-ET Center. Patient(s) Couples with male factor infertility undergoing ICSI using ejaculated semen. Intervention(s) The patients were prospectively randomized according to differences in sperm preincubation time before ICSI into 1-hour, 3-hour, and 5-hour groups. The status of the acrosome was studied using electron microscopy. Main Outcome Measure(s) The primary outcome measures were fertilization rate and acrosome reaction rate. Secondary outcome measures were the implantation and pregnancy rates. Result(s) The rate of acrosomally reacted spermatozoa was the highest (68.2%) after 5 hours of incubation and lowest (25.6%) after 1 hour of incubation. The difference was statistically significant. The fertilization rate was the highest (74%) using spermatozoa incubated for 3 hours as compared with 1 hour (70%) and 5 hours (67%), but the difference was not statistically significant. Conclusion(s) Acrosome reaction is time dependent; the optimum incubation time of spermatozoa before ICSI was 3 hours, which resulted in the highest fertilization rate.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>18295761</pmid><doi>10.1016/j.fertnstert.2006.11.176</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Fertility and sterility, 2008-09, Vol.90 (3), p.584-591 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Free E-Journal (出版社公開部分のみ）; Alma/SFX Local Collection |
| subjects | Acrosome Reaction Adult Biological and medical sciences Egypt - epidemiology electron microscopy Female fertilization Gynecology. Andrology. Obstetrics Humans ICSI Infertility, Male - epidemiology Infertility, Male - therapy Internal Medicine Male Medical sciences Obstetrics and Gynecology Pregnancy Pregnancy Rate Semen Preservation - methods Semen Preservation - statistics & numerical data Sperm Injections, Intracytoplasmic - statistics & numerical data sperm preincubation time Time Factors Treatment Outcome |
| title | The impact of spermatozoa preincubation time and spontaneous acrosome reaction in intracytoplasmic sperm injection: a controlled randomized study |
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