IS3 peptide-formed ion channels in rat skeletal muscle cell membranes
A 22-mer peptide, identical to the primary sequence of domain I segment 3 (IS3) of rat brain sodium channel I, was synthesized. With the patch clamp cell-attached technique, single channel currents could be recorded from the patches of cultured rat myotube membranes when the patches were held at hyp...
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| Veröffentlicht in: | FEBS letters 1999-03, Vol.446 (2), p.351-354 |
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| container_title | FEBS letters |
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| creator | Bao, Lin Miao, Zhen-Wei Zhou, Pei-Ai Jiang, Yun Sha, Yin-Lin Zhang, Ren-Ji Tang, You-Chi |
| description | A 22-mer peptide, identical to the primary sequence of domain I segment 3 (IS3) of rat brain sodium channel I, was synthesized. With the patch clamp cell-attached technique, single channel currents could be recorded from the patches of cultured rat myotube membranes when the patches were held at hyperpolarized potentials and the electrode solution contained NaCl and 1 μM IS3, indicating that IS3 incorporated into the membranes and formed ion channels. The single channel conductances of IS3 channels were distributed heterogeneously, but mainly in the range of 10–25 pS. There was a tendency that the mean open time and open probability of IS3 channels increased and the mean close time decreased with the increasing of hyperpolarized membrane potentials. IS3 channels are highly selective for Na
+ and Li
+ but not for Cl
− and K
+, similar to the authentic Na
+ channels. |
| doi_str_mv | 10.1016/S0014-5793(99)00249-5 |
| format | Article |
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+ and Li
+ but not for Cl
− and K
+, similar to the authentic Na
+ channels.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/S0014-5793(99)00249-5</identifier><identifier>PMID: 10100872</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Animals ; Cell Membrane - physiology ; Cultured rat myotube membrane ; IS3 channel ; IS3 peptide, a synthetic peptide corresponding to the transmembrane segment 3 of repeat I from rat brain Na+ channel type I ; Membrane Potentials ; Molecular Sequence Data ; Muscle, Skeletal - cytology ; Na + selective channel ; Peptides - physiology ; Rats ; Rats, Sprague-Dawley ; Single channel recording ; Sodium Channels - physiology ; Synthetic peptide</subject><ispartof>FEBS letters, 1999-03, Vol.446 (2), p.351-354</ispartof><rights>1999 Federation of European Biochemical Societies</rights><rights>FEBS Letters 446 (1999) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3745-8c82ea3dc9474c4fbb91197669d7efd35e14225a5eb42ead9c9389ecf00216cf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0014-5793%2899%2900249-5$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0014-5793(99)00249-5$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,777,781,1412,1428,3537,27905,27906,45555,45556,45976,46390,46814</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10100872$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bao, Lin</creatorcontrib><creatorcontrib>Miao, Zhen-Wei</creatorcontrib><creatorcontrib>Zhou, Pei-Ai</creatorcontrib><creatorcontrib>Jiang, Yun</creatorcontrib><creatorcontrib>Sha, Yin-Lin</creatorcontrib><creatorcontrib>Zhang, Ren-Ji</creatorcontrib><creatorcontrib>Tang, You-Chi</creatorcontrib><title>IS3 peptide-formed ion channels in rat skeletal muscle cell membranes</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>A 22-mer peptide, identical to the primary sequence of domain I segment 3 (IS3) of rat brain sodium channel I, was synthesized. With the patch clamp cell-attached technique, single channel currents could be recorded from the patches of cultured rat myotube membranes when the patches were held at hyperpolarized potentials and the electrode solution contained NaCl and 1 μM IS3, indicating that IS3 incorporated into the membranes and formed ion channels. The single channel conductances of IS3 channels were distributed heterogeneously, but mainly in the range of 10–25 pS. There was a tendency that the mean open time and open probability of IS3 channels increased and the mean close time decreased with the increasing of hyperpolarized membrane potentials. IS3 channels are highly selective for Na
+ and Li
+ but not for Cl
− and K
+, similar to the authentic Na
+ channels.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Cell Membrane - physiology</subject><subject>Cultured rat myotube membrane</subject><subject>IS3 channel</subject><subject>IS3 peptide, a synthetic peptide corresponding to the transmembrane segment 3 of repeat I from rat brain Na+ channel type I</subject><subject>Membrane Potentials</subject><subject>Molecular Sequence Data</subject><subject>Muscle, Skeletal - cytology</subject><subject>Na + selective channel</subject><subject>Peptides - physiology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Single channel recording</subject><subject>Sodium Channels - physiology</subject><subject>Synthetic peptide</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMtOwzAQRS0EouXxCaCsECwCdmwnnhUCVGglJBbA2kqciTDkUewUxN_jNBViByvbM3fuXB9Cjhg9Z5SlF4-UMhHLDPgpwBmliYBYbpEpUxmPuUjVNpn-SCZkz_tXGt6KwS6ZBAdKVZZMyWzxyKMlLntbYlx1rsEysl0bmZe8bbH2kW0jl_eRf8Ma-7yOmpU3NUYG63DHpnB5i_6A7FR57fFwc-6T59vZ0808vn-4W9xc3ceGZ0LGyqgEc14aEJkwoioKYAyyNIUyw6rkEplIEplLLEQQlmCAK0BThe-x1FR8n5yMvkvXva_Q97qxfogSQnQrr1NIJSRKBaEchcZ13jus9NLZJndfmlE98NNrfnqAowH0mp-WYe54s2BVBBS_pkZgQTAfBZ-2xq__uerb2XWy7gwNgHV52HU5WgXM-GHRaW8stgZL69D0uuzsH2m_AR0kkwo</recordid><startdate>19990312</startdate><enddate>19990312</enddate><creator>Bao, Lin</creator><creator>Miao, Zhen-Wei</creator><creator>Zhou, Pei-Ai</creator><creator>Jiang, Yun</creator><creator>Sha, Yin-Lin</creator><creator>Zhang, Ren-Ji</creator><creator>Tang, You-Chi</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990312</creationdate><title>IS3 peptide-formed ion channels in rat skeletal muscle cell membranes</title><author>Bao, Lin ; Miao, Zhen-Wei ; Zhou, Pei-Ai ; Jiang, Yun ; Sha, Yin-Lin ; Zhang, Ren-Ji ; Tang, You-Chi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3745-8c82ea3dc9474c4fbb91197669d7efd35e14225a5eb42ead9c9389ecf00216cf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Cell Membrane - physiology</topic><topic>Cultured rat myotube membrane</topic><topic>IS3 channel</topic><topic>IS3 peptide, a synthetic peptide corresponding to the transmembrane segment 3 of repeat I from rat brain Na+ channel type I</topic><topic>Membrane Potentials</topic><topic>Molecular Sequence Data</topic><topic>Muscle, Skeletal - cytology</topic><topic>Na + selective channel</topic><topic>Peptides - physiology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Single channel recording</topic><topic>Sodium Channels - physiology</topic><topic>Synthetic peptide</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bao, Lin</creatorcontrib><creatorcontrib>Miao, Zhen-Wei</creatorcontrib><creatorcontrib>Zhou, Pei-Ai</creatorcontrib><creatorcontrib>Jiang, Yun</creatorcontrib><creatorcontrib>Sha, Yin-Lin</creatorcontrib><creatorcontrib>Zhang, Ren-Ji</creatorcontrib><creatorcontrib>Tang, You-Chi</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bao, Lin</au><au>Miao, Zhen-Wei</au><au>Zhou, Pei-Ai</au><au>Jiang, Yun</au><au>Sha, Yin-Lin</au><au>Zhang, Ren-Ji</au><au>Tang, You-Chi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>IS3 peptide-formed ion channels in rat skeletal muscle cell membranes</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1999-03-12</date><risdate>1999</risdate><volume>446</volume><issue>2</issue><spage>351</spage><epage>354</epage><pages>351-354</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>A 22-mer peptide, identical to the primary sequence of domain I segment 3 (IS3) of rat brain sodium channel I, was synthesized. With the patch clamp cell-attached technique, single channel currents could be recorded from the patches of cultured rat myotube membranes when the patches were held at hyperpolarized potentials and the electrode solution contained NaCl and 1 μM IS3, indicating that IS3 incorporated into the membranes and formed ion channels. The single channel conductances of IS3 channels were distributed heterogeneously, but mainly in the range of 10–25 pS. There was a tendency that the mean open time and open probability of IS3 channels increased and the mean close time decreased with the increasing of hyperpolarized membrane potentials. IS3 channels are highly selective for Na
+ and Li
+ but not for Cl
− and K
+, similar to the authentic Na
+ channels.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>10100872</pmid><doi>10.1016/S0014-5793(99)00249-5</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Free Content; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Animals Cell Membrane - physiology Cultured rat myotube membrane IS3 channel IS3 peptide, a synthetic peptide corresponding to the transmembrane segment 3 of repeat I from rat brain Na+ channel type I Membrane Potentials Molecular Sequence Data Muscle, Skeletal - cytology Na + selective channel Peptides - physiology Rats Rats, Sprague-Dawley Single channel recording Sodium Channels - physiology Synthetic peptide |
| title | IS3 peptide-formed ion channels in rat skeletal muscle cell membranes |
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