Cloning of the mouse phospholipid hydroperoxide glutathione peroxidase gene
15-Lipoxygenases and phospholipid hydroperoxide glutathione peroxidases (PH-GPx) are counterparts in the metabolism of hydroperoxy lipids and a balanced regulation of both enzymes appears to be important for the cellular peroxide tone regulating the expression of redox sensitive genes. In contrast t...
Gespeichert in:
| Veröffentlicht in: | FEBS letters 1999-03, Vol.446 (2), p.223-227 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 227 |
|---|---|
| container_issue | 2 |
| container_start_page | 223 |
| container_title | FEBS letters |
| container_volume | 446 |
| creator | Borchert, Astrid Schnurr, Kerstin Thiele, Bernd J Kühn, Hartmut |
| description | 15-Lipoxygenases and phospholipid hydroperoxide glutathione peroxidases (PH-GPx) are counterparts in the metabolism of hydroperoxy lipids and a balanced regulation of both enzymes appears to be important for the cellular peroxide tone regulating the expression of redox sensitive genes. In contrast to lipoxygenases the molecular biology of PH-GPx is less well investigated. In this study we cloned the PH-GPx cDNA from a mouse fibroblast cDNA library and the PH-GPx gene from a mouse genomic library. The gene spans approximately 4 kb which includes 1 kb of 5′-flanking region and consists of seven exons and six introns. The immediate promoter region does not contain a TATA box but there are binding sites for several transcription factors which also occur in the porcine gene. Our investigations provide useful tools for future targeted gene disruption studies. |
| doi_str_mv | 10.1016/S0014-5793(99)00221-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_69659045</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0014579399002215</els_id><sourcerecordid>69659045</sourcerecordid><originalsourceid>FETCH-LOGICAL-e263t-a288ad680c311d3ddbf4f1fd381f71d67899358978a16fe60472c0db9218b3d53</originalsourceid><addsrcrecordid>eNo9kE1LxDAQhoMo7rr6E5SeRA_VpGnT5CTL4hcueFDPIW2m20jb1KQV99-b_dDDMMzwzMs7L0LnBN8QTNjtG8YkjbNc0CshrjFOEhJnB2hKeE5jmjJ-iKb_yASdeP-Jw8yJOEaToIAxT7Mpelk0tjPdKrJVNNQQtXb0EPW19aEa0xsd1WvtbA_O_hgN0aoZBzXUxnYB2y1VuFhBB6foqFKNh7N9n6GPh_v3xVO8fH18XsyXMSSMDrFKOFeacVxSQjTVuqjSilSaclLlRLOcC0EzLnKuCKuA4TRPSqwLkRBeUJ3RGbrc6fbOfo3gB9kaX0LTqA6CfckEywRON-DFHhyLFrTsnWmVW8u_9wNwtwMg2P024KQvDXQlaOOgHKS2JsAbnslt4HKTphRCbgOXGf0F8c9x-w</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69659045</pqid></control><display><type>article</type><title>Cloning of the mouse phospholipid hydroperoxide glutathione peroxidase gene</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Access via Wiley Online Library</source><source>Wiley Free Content</source><source>Alma/SFX Local Collection</source><creator>Borchert, Astrid ; Schnurr, Kerstin ; Thiele, Bernd J ; Kühn, Hartmut</creator><creatorcontrib>Borchert, Astrid ; Schnurr, Kerstin ; Thiele, Bernd J ; Kühn, Hartmut</creatorcontrib><description>15-Lipoxygenases and phospholipid hydroperoxide glutathione peroxidases (PH-GPx) are counterparts in the metabolism of hydroperoxy lipids and a balanced regulation of both enzymes appears to be important for the cellular peroxide tone regulating the expression of redox sensitive genes. In contrast to lipoxygenases the molecular biology of PH-GPx is less well investigated. In this study we cloned the PH-GPx cDNA from a mouse fibroblast cDNA library and the PH-GPx gene from a mouse genomic library. The gene spans approximately 4 kb which includes 1 kb of 5′-flanking region and consists of seven exons and six introns. The immediate promoter region does not contain a TATA box but there are binding sites for several transcription factors which also occur in the porcine gene. Our investigations provide useful tools for future targeted gene disruption studies.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/S0014-5793(99)00221-5</identifier><identifier>PMID: 10100845</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Animals ; Atherogenesis ; Base Sequence ; Cloning, Molecular ; DNA, Complementary ; Glutathione Peroxidase - genetics ; Humans ; Inflammation ; Lipid peroxidation ; Mice ; Molecular Sequence Data ; Oxidative stress ; Selenium ; Sequence Homology, Nucleic Acid ; Swine</subject><ispartof>FEBS letters, 1999-03, Vol.446 (2), p.223-227</ispartof><rights>1999 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0014-5793(99)00221-5$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10100845$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Borchert, Astrid</creatorcontrib><creatorcontrib>Schnurr, Kerstin</creatorcontrib><creatorcontrib>Thiele, Bernd J</creatorcontrib><creatorcontrib>Kühn, Hartmut</creatorcontrib><title>Cloning of the mouse phospholipid hydroperoxide glutathione peroxidase gene</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>15-Lipoxygenases and phospholipid hydroperoxide glutathione peroxidases (PH-GPx) are counterparts in the metabolism of hydroperoxy lipids and a balanced regulation of both enzymes appears to be important for the cellular peroxide tone regulating the expression of redox sensitive genes. In contrast to lipoxygenases the molecular biology of PH-GPx is less well investigated. In this study we cloned the PH-GPx cDNA from a mouse fibroblast cDNA library and the PH-GPx gene from a mouse genomic library. The gene spans approximately 4 kb which includes 1 kb of 5′-flanking region and consists of seven exons and six introns. The immediate promoter region does not contain a TATA box but there are binding sites for several transcription factors which also occur in the porcine gene. Our investigations provide useful tools for future targeted gene disruption studies.</description><subject>Animals</subject><subject>Atherogenesis</subject><subject>Base Sequence</subject><subject>Cloning, Molecular</subject><subject>DNA, Complementary</subject><subject>Glutathione Peroxidase - genetics</subject><subject>Humans</subject><subject>Inflammation</subject><subject>Lipid peroxidation</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>Oxidative stress</subject><subject>Selenium</subject><subject>Sequence Homology, Nucleic Acid</subject><subject>Swine</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1LxDAQhoMo7rr6E5SeRA_VpGnT5CTL4hcueFDPIW2m20jb1KQV99-b_dDDMMzwzMs7L0LnBN8QTNjtG8YkjbNc0CshrjFOEhJnB2hKeE5jmjJ-iKb_yASdeP-Jw8yJOEaToIAxT7Mpelk0tjPdKrJVNNQQtXb0EPW19aEa0xsd1WvtbA_O_hgN0aoZBzXUxnYB2y1VuFhBB6foqFKNh7N9n6GPh_v3xVO8fH18XsyXMSSMDrFKOFeacVxSQjTVuqjSilSaclLlRLOcC0EzLnKuCKuA4TRPSqwLkRBeUJ3RGbrc6fbOfo3gB9kaX0LTqA6CfckEywRON-DFHhyLFrTsnWmVW8u_9wNwtwMg2P024KQvDXQlaOOgHKS2JsAbnslt4HKTphRCbgOXGf0F8c9x-w</recordid><startdate>19990312</startdate><enddate>19990312</enddate><creator>Borchert, Astrid</creator><creator>Schnurr, Kerstin</creator><creator>Thiele, Bernd J</creator><creator>Kühn, Hartmut</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19990312</creationdate><title>Cloning of the mouse phospholipid hydroperoxide glutathione peroxidase gene</title><author>Borchert, Astrid ; Schnurr, Kerstin ; Thiele, Bernd J ; Kühn, Hartmut</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e263t-a288ad680c311d3ddbf4f1fd381f71d67899358978a16fe60472c0db9218b3d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Atherogenesis</topic><topic>Base Sequence</topic><topic>Cloning, Molecular</topic><topic>DNA, Complementary</topic><topic>Glutathione Peroxidase - genetics</topic><topic>Humans</topic><topic>Inflammation</topic><topic>Lipid peroxidation</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>Oxidative stress</topic><topic>Selenium</topic><topic>Sequence Homology, Nucleic Acid</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Borchert, Astrid</creatorcontrib><creatorcontrib>Schnurr, Kerstin</creatorcontrib><creatorcontrib>Thiele, Bernd J</creatorcontrib><creatorcontrib>Kühn, Hartmut</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Borchert, Astrid</au><au>Schnurr, Kerstin</au><au>Thiele, Bernd J</au><au>Kühn, Hartmut</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning of the mouse phospholipid hydroperoxide glutathione peroxidase gene</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1999-03-12</date><risdate>1999</risdate><volume>446</volume><issue>2</issue><spage>223</spage><epage>227</epage><pages>223-227</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>15-Lipoxygenases and phospholipid hydroperoxide glutathione peroxidases (PH-GPx) are counterparts in the metabolism of hydroperoxy lipids and a balanced regulation of both enzymes appears to be important for the cellular peroxide tone regulating the expression of redox sensitive genes. In contrast to lipoxygenases the molecular biology of PH-GPx is less well investigated. In this study we cloned the PH-GPx cDNA from a mouse fibroblast cDNA library and the PH-GPx gene from a mouse genomic library. The gene spans approximately 4 kb which includes 1 kb of 5′-flanking region and consists of seven exons and six introns. The immediate promoter region does not contain a TATA box but there are binding sites for several transcription factors which also occur in the porcine gene. Our investigations provide useful tools for future targeted gene disruption studies.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>10100845</pmid><doi>10.1016/S0014-5793(99)00221-5</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1999-03, Vol.446 (2), p.223-227 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69659045 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Access via Wiley Online Library; Wiley Free Content; Alma/SFX Local Collection |
| subjects | Animals Atherogenesis Base Sequence Cloning, Molecular DNA, Complementary Glutathione Peroxidase - genetics Humans Inflammation Lipid peroxidation Mice Molecular Sequence Data Oxidative stress Selenium Sequence Homology, Nucleic Acid Swine |
| title | Cloning of the mouse phospholipid hydroperoxide glutathione peroxidase gene |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-21T09%3A38%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cloning%20of%20the%20mouse%20phospholipid%20hydroperoxide%20glutathione%20peroxidase%20gene&rft.jtitle=FEBS%20letters&rft.au=Borchert,%20Astrid&rft.date=1999-03-12&rft.volume=446&rft.issue=2&rft.spage=223&rft.epage=227&rft.pages=223-227&rft.issn=0014-5793&rft.eissn=1873-3468&rft_id=info:doi/10.1016/S0014-5793(99)00221-5&rft_dat=%3Cproquest_pubme%3E69659045%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=69659045&rft_id=info:pmid/10100845&rft_els_id=S0014579399002215&rfr_iscdi=true |