Pdr12p-dependent and -independent fluorescein extrusion from baker's yeast cells
Fluorescein efflux from S. cerevisiae cells was measured to study the peculiarities of fluorescein transport system, which is important for yeast resistance to certain drugs and weak organic acid preservatives. Glucose-independent and glucose-stimulated fluorescein effluxes were characterized using...
Gespeichert in:
| Veröffentlicht in: | Acta biochimica polonica 2008-01, Vol.55 (3), p.595-601 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 601 |
|---|---|
| container_issue | 3 |
| container_start_page | 595 |
| container_title | Acta biochimica polonica |
| container_volume | 55 |
| creator | Lushchak, Volodymyr Abrat, Oleksandra Miedzobrodzki, Jacek Semchyshyn, Halyna |
| description | Fluorescein efflux from S. cerevisiae cells was measured to study the peculiarities of fluorescein transport system, which is important for yeast resistance to certain drugs and weak organic acid preservatives. Glucose-independent and glucose-stimulated fluorescein effluxes were characterized using iodoacetate, cyanide and orthovanadate, inhibitors of glycolysis, electron transport chain, and ATPases, respectively. It is supposed that in glucose-free medium fluorescein extrusion is ATP-dependent and the energy for this efflux is mainly provided by respiration. In glucose-containing medium, glycolysis plays a critical role for extrusion of fluorescein. The results indicate that acetic acid inhibits the fluorescein efflux from yeast cells. The inhibition constant of glucose-stimulated fluorescein efflux is significantly lower in parental strain than in two mutants defective in PDR12 (ABC-transporter Pdr12p) or WAR1 (transcription factor of Pdr12p). It can be suggested that the membrane protein Pdr12 is involved in fluorescein extrusion from the yeast cells, but component(s) other than Pdr12p is (are) also important. |
| doi_str_mv | 10.18388/abp.2008_3066 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69657926</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69657926</sourcerecordid><originalsourceid>FETCH-LOGICAL-c364t-abbc5182be5658ae08118ba220e2b39103158c52f8a3ccc46d55a443668a8bb93</originalsourceid><addsrcrecordid>eNqFkEtLw0AURgdRbKxuXUpWupo670yWUnxBwS4UuhtmJjcQzcuZBOy_N9pil64u3Hu-j8tB6JKSBdVc61vr-gUjRBtOlDpCCc24wFSKzTFKCCEUS5ZtZugsxndCGKe5OEUzqvV0ymSC1usiUNbjAnpoC2iH1LZFiqv2sCjrsQsQPVRtCl9DGGPVtWkZuiZ19gPCTUy3YOOQeqjreI5OSltHuNjPOXp7uH9dPuHVy-Pz8m6FPVdiwNY5L6lmDqSS2gLRlGpnGSPAHM8p4VRqL1mpLffeC1VIaYXgSmmrncv5HF3vevvQfY4QB9NU8ecD20I3RqNyJbOcqX9BRkQmmcgmcLEDfehiDFCaPlSNDVtDifmVbSbZ5k_2FLjaN4-ugeKA7-3ybxzeeqQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20475247</pqid></control><display><type>article</type><title>Pdr12p-dependent and -independent fluorescein extrusion from baker's yeast cells</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Free Full-Text Journals in Chemistry</source><creator>Lushchak, Volodymyr ; Abrat, Oleksandra ; Miedzobrodzki, Jacek ; Semchyshyn, Halyna</creator><creatorcontrib>Lushchak, Volodymyr ; Abrat, Oleksandra ; Miedzobrodzki, Jacek ; Semchyshyn, Halyna</creatorcontrib><description>Fluorescein efflux from S. cerevisiae cells was measured to study the peculiarities of fluorescein transport system, which is important for yeast resistance to certain drugs and weak organic acid preservatives. Glucose-independent and glucose-stimulated fluorescein effluxes were characterized using iodoacetate, cyanide and orthovanadate, inhibitors of glycolysis, electron transport chain, and ATPases, respectively. It is supposed that in glucose-free medium fluorescein extrusion is ATP-dependent and the energy for this efflux is mainly provided by respiration. In glucose-containing medium, glycolysis plays a critical role for extrusion of fluorescein. The results indicate that acetic acid inhibits the fluorescein efflux from yeast cells. The inhibition constant of glucose-stimulated fluorescein efflux is significantly lower in parental strain than in two mutants defective in PDR12 (ABC-transporter Pdr12p) or WAR1 (transcription factor of Pdr12p). It can be suggested that the membrane protein Pdr12 is involved in fluorescein extrusion from the yeast cells, but component(s) other than Pdr12p is (are) also important.</description><identifier>ISSN: 0001-527X</identifier><identifier>EISSN: 1734-154X</identifier><identifier>DOI: 10.18388/abp.2008_3066</identifier><identifier>PMID: 18800175</identifier><language>eng</language><publisher>Poland</publisher><subject>Acetic Acid - pharmacology ; ATP-Binding Cassette Transporters - genetics ; ATP-Binding Cassette Transporters - metabolism ; Biological Transport, Active - drug effects ; Cyanides - pharmacology ; Energy Metabolism ; Fluorescein - pharmacokinetics ; Fluorescent Dyes - pharmacokinetics ; Genes, Fungal ; Glucose - metabolism ; Glucose - pharmacology ; Iodoacetates - pharmacology ; Mutation ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - drug effects ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Saccharomyces cerevisiae Proteins - genetics ; Saccharomyces cerevisiae Proteins - metabolism ; Stress, Physiological ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Vanadates - pharmacology</subject><ispartof>Acta biochimica polonica, 2008-01, Vol.55 (3), p.595-601</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c364t-abbc5182be5658ae08118ba220e2b39103158c52f8a3ccc46d55a443668a8bb93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,862,27907,27908</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18800175$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lushchak, Volodymyr</creatorcontrib><creatorcontrib>Abrat, Oleksandra</creatorcontrib><creatorcontrib>Miedzobrodzki, Jacek</creatorcontrib><creatorcontrib>Semchyshyn, Halyna</creatorcontrib><title>Pdr12p-dependent and -independent fluorescein extrusion from baker's yeast cells</title><title>Acta biochimica polonica</title><addtitle>Acta Biochim Pol</addtitle><description>Fluorescein efflux from S. cerevisiae cells was measured to study the peculiarities of fluorescein transport system, which is important for yeast resistance to certain drugs and weak organic acid preservatives. Glucose-independent and glucose-stimulated fluorescein effluxes were characterized using iodoacetate, cyanide and orthovanadate, inhibitors of glycolysis, electron transport chain, and ATPases, respectively. It is supposed that in glucose-free medium fluorescein extrusion is ATP-dependent and the energy for this efflux is mainly provided by respiration. In glucose-containing medium, glycolysis plays a critical role for extrusion of fluorescein. The results indicate that acetic acid inhibits the fluorescein efflux from yeast cells. The inhibition constant of glucose-stimulated fluorescein efflux is significantly lower in parental strain than in two mutants defective in PDR12 (ABC-transporter Pdr12p) or WAR1 (transcription factor of Pdr12p). It can be suggested that the membrane protein Pdr12 is involved in fluorescein extrusion from the yeast cells, but component(s) other than Pdr12p is (are) also important.</description><subject>Acetic Acid - pharmacology</subject><subject>ATP-Binding Cassette Transporters - genetics</subject><subject>ATP-Binding Cassette Transporters - metabolism</subject><subject>Biological Transport, Active - drug effects</subject><subject>Cyanides - pharmacology</subject><subject>Energy Metabolism</subject><subject>Fluorescein - pharmacokinetics</subject><subject>Fluorescent Dyes - pharmacokinetics</subject><subject>Genes, Fungal</subject><subject>Glucose - metabolism</subject><subject>Glucose - pharmacology</subject><subject>Iodoacetates - pharmacology</subject><subject>Mutation</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - drug effects</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Saccharomyces cerevisiae Proteins - genetics</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><subject>Stress, Physiological</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Vanadates - pharmacology</subject><issn>0001-527X</issn><issn>1734-154X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLw0AURgdRbKxuXUpWupo670yWUnxBwS4UuhtmJjcQzcuZBOy_N9pil64u3Hu-j8tB6JKSBdVc61vr-gUjRBtOlDpCCc24wFSKzTFKCCEUS5ZtZugsxndCGKe5OEUzqvV0ymSC1usiUNbjAnpoC2iH1LZFiqv2sCjrsQsQPVRtCl9DGGPVtWkZuiZ19gPCTUy3YOOQeqjreI5OSltHuNjPOXp7uH9dPuHVy-Pz8m6FPVdiwNY5L6lmDqSS2gLRlGpnGSPAHM8p4VRqL1mpLffeC1VIaYXgSmmrncv5HF3vevvQfY4QB9NU8ecD20I3RqNyJbOcqX9BRkQmmcgmcLEDfehiDFCaPlSNDVtDifmVbSbZ5k_2FLjaN4-ugeKA7-3ybxzeeqQ</recordid><startdate>20080101</startdate><enddate>20080101</enddate><creator>Lushchak, Volodymyr</creator><creator>Abrat, Oleksandra</creator><creator>Miedzobrodzki, Jacek</creator><creator>Semchyshyn, Halyna</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20080101</creationdate><title>Pdr12p-dependent and -independent fluorescein extrusion from baker's yeast cells</title><author>Lushchak, Volodymyr ; Abrat, Oleksandra ; Miedzobrodzki, Jacek ; Semchyshyn, Halyna</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c364t-abbc5182be5658ae08118ba220e2b39103158c52f8a3ccc46d55a443668a8bb93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Acetic Acid - pharmacology</topic><topic>ATP-Binding Cassette Transporters - genetics</topic><topic>ATP-Binding Cassette Transporters - metabolism</topic><topic>Biological Transport, Active - drug effects</topic><topic>Cyanides - pharmacology</topic><topic>Energy Metabolism</topic><topic>Fluorescein - pharmacokinetics</topic><topic>Fluorescent Dyes - pharmacokinetics</topic><topic>Genes, Fungal</topic><topic>Glucose - metabolism</topic><topic>Glucose - pharmacology</topic><topic>Iodoacetates - pharmacology</topic><topic>Mutation</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - drug effects</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Saccharomyces cerevisiae Proteins - genetics</topic><topic>Saccharomyces cerevisiae Proteins - metabolism</topic><topic>Stress, Physiological</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Vanadates - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lushchak, Volodymyr</creatorcontrib><creatorcontrib>Abrat, Oleksandra</creatorcontrib><creatorcontrib>Miedzobrodzki, Jacek</creatorcontrib><creatorcontrib>Semchyshyn, Halyna</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Acta biochimica polonica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lushchak, Volodymyr</au><au>Abrat, Oleksandra</au><au>Miedzobrodzki, Jacek</au><au>Semchyshyn, Halyna</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pdr12p-dependent and -independent fluorescein extrusion from baker's yeast cells</atitle><jtitle>Acta biochimica polonica</jtitle><addtitle>Acta Biochim Pol</addtitle><date>2008-01-01</date><risdate>2008</risdate><volume>55</volume><issue>3</issue><spage>595</spage><epage>601</epage><pages>595-601</pages><issn>0001-527X</issn><eissn>1734-154X</eissn><abstract>Fluorescein efflux from S. cerevisiae cells was measured to study the peculiarities of fluorescein transport system, which is important for yeast resistance to certain drugs and weak organic acid preservatives. Glucose-independent and glucose-stimulated fluorescein effluxes were characterized using iodoacetate, cyanide and orthovanadate, inhibitors of glycolysis, electron transport chain, and ATPases, respectively. It is supposed that in glucose-free medium fluorescein extrusion is ATP-dependent and the energy for this efflux is mainly provided by respiration. In glucose-containing medium, glycolysis plays a critical role for extrusion of fluorescein. The results indicate that acetic acid inhibits the fluorescein efflux from yeast cells. The inhibition constant of glucose-stimulated fluorescein efflux is significantly lower in parental strain than in two mutants defective in PDR12 (ABC-transporter Pdr12p) or WAR1 (transcription factor of Pdr12p). It can be suggested that the membrane protein Pdr12 is involved in fluorescein extrusion from the yeast cells, but component(s) other than Pdr12p is (are) also important.</abstract><cop>Poland</cop><pmid>18800175</pmid><doi>10.18388/abp.2008_3066</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0001-527X |
| ispartof | Acta biochimica polonica, 2008-01, Vol.55 (3), p.595-601 |
| issn | 0001-527X 1734-154X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69657926 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Free Full-Text Journals in Chemistry |
| subjects | Acetic Acid - pharmacology ATP-Binding Cassette Transporters - genetics ATP-Binding Cassette Transporters - metabolism Biological Transport, Active - drug effects Cyanides - pharmacology Energy Metabolism Fluorescein - pharmacokinetics Fluorescent Dyes - pharmacokinetics Genes, Fungal Glucose - metabolism Glucose - pharmacology Iodoacetates - pharmacology Mutation Saccharomyces cerevisiae Saccharomyces cerevisiae - drug effects Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Stress, Physiological Transcription Factors - genetics Transcription Factors - metabolism Vanadates - pharmacology |
| title | Pdr12p-dependent and -independent fluorescein extrusion from baker's yeast cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T12%3A11%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Pdr12p-dependent%20and%20-independent%20fluorescein%20extrusion%20from%20baker's%20yeast%20cells&rft.jtitle=Acta%20biochimica%20polonica&rft.au=Lushchak,%20Volodymyr&rft.date=2008-01-01&rft.volume=55&rft.issue=3&rft.spage=595&rft.epage=601&rft.pages=595-601&rft.issn=0001-527X&rft.eissn=1734-154X&rft_id=info:doi/10.18388/abp.2008_3066&rft_dat=%3Cproquest_cross%3E69657926%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=20475247&rft_id=info:pmid/18800175&rfr_iscdi=true |