Charge-modified single chain antibody constructs of monoclonal antibody CC49: generation, characterization, pharmacokinetics, and biodistribution analysis

A novel strategy was developed in which an antibody scFv fragment of the monoclonal antibody (MAb) CC49 was modified by engineering DNA coding sequences to lower its isoelectric point. Negatively charged amino acids were added to the carboxy terminus of the CC49 V H region by adding nucleotide seque...

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Veröffentlicht in:	Nuclear medicine and biology 1999, Vol.26 (1), p.27-34
Hauptverfasser:	Pavlinkova, Gabriela, Beresford, Guy, Booth, Barbara J.M, Batra, Surinder K, Colcher, David
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Antibodies, Neoplasm - biosynthesis Antibodies, Neoplasm - metabolism Antibody engineering Antibody Formation Base Sequence Biological and medical sciences Carcinoma - metabolism Colon carcinoma xenografts Colonic Neoplasms - metabolism Digestive system Female Humans Immunoglobulin Variable Region Investigative techniques, diagnostic techniques (general aspects) Iodine Radioisotopes Isoelectric Point Medical sciences Mice Mice, Nude Molecular Sequence Data Neoplasm Transplantation Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Polymerase Chain Reaction Radioimmunodiagnosis Radiopharmaceuticals Single chain fragments
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