Expression and one-step purification of a fully active polyhistidine-tagged cytochrome bc1 complex from Rhodobacter sphaeroides

The fbcB and fbcC genes encoding cytochromes b and c1 of the bc1 complex were extended with a segment to encode a polyhistidine tag linked to their C-terminal sequence allowing a one-step affinity purification of the complex. Constructions were made in vitro in a pUC-derived background using PCR amp...

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Veröffentlicht in:	Protein expression and purification 1999-04, Vol.15 (3), p.370-380
Hauptverfasser:	Guergova-Kuras, M, Salcedo-Hernandez, R, Bechmann, G, Kuras, R, Gennis, R B, Crofts, A R
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Base Sequence Chromatography, Affinity Chromatography, Gel Cloning, Molecular Cytochrome b Group - chemistry Cytochrome b Group - genetics Cytochromes c1 - chemistry Cytochromes c1 - genetics DNA Primers Electron Transport Complex III - genetics Electron Transport Complex III - isolation & purification Electron Transport Complex III - metabolism Electrophoresis, Polyacrylamide Gel Escherichia coli Histidine Molecular Sequence Data Peptides Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Rhodobacter sphaeroides - enzymology Rhodobacter sphaeroides - genetics
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