Intraepidermal Expression of Basement Membrane Components in the Lesional Skin of a Patient with Dystrophic Epidermolysis Bullosa
The patient was a 15‐year‐old male. Since birth, he had developed blistering and erosion of the skin. Biopsy skin specimen of the bullous lesions showed subepidermal blister formation. Electron microscopic examination revealed that tissue separation had occurred at the sublamina densa level. By indi...
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| Veröffentlicht in: | Journal of dermatology 1999-02, Vol.26 (2), p.106-110 |
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| container_title | Journal of dermatology |
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| creator | Muramatsu, Tsutomu Ko, Toshiko Honoki, Kumiko Hatoko, Mituso Shirai, Toshihiko Vnittanakom, Pramote |
| description | The patient was a 15‐year‐old male. Since birth, he had developed blistering and erosion of the skin. Biopsy skin specimen of the bullous lesions showed subepidermal blister formation. Electron microscopic examination revealed that tissue separation had occurred at the sublamina densa level. By indirect immunofluorescence using antibodies specific for α6 integrin, laminin 5, type IV collagen, and type VII collagen, all of these basement membrane components were detected as coarse granular intracytoplasmic deposits only in the basal and suprabasal cells of the blister roof. In the non‐blistered regions, these basement membrane components showed a linear pattern similar to that seen in normal skin. These findings suggest that intraepidermal expression of basement membrane components was closely related to the blister formation. The biological meaning of intraepidermal expression of basement membrane components were also discussed. |
| doi_str_mv | 10.1111/j.1346-8138.1999.tb03519.x |
| format | Article |
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Since birth, he had developed blistering and erosion of the skin. Biopsy skin specimen of the bullous lesions showed subepidermal blister formation. Electron microscopic examination revealed that tissue separation had occurred at the sublamina densa level. By indirect immunofluorescence using antibodies specific for α6 integrin, laminin 5, type IV collagen, and type VII collagen, all of these basement membrane components were detected as coarse granular intracytoplasmic deposits only in the basal and suprabasal cells of the blister roof. In the non‐blistered regions, these basement membrane components showed a linear pattern similar to that seen in normal skin. These findings suggest that intraepidermal expression of basement membrane components was closely related to the blister formation. The biological meaning of intraepidermal expression of basement membrane components were also discussed.</description><identifier>ISSN: 0385-2407</identifier><identifier>EISSN: 1346-8138</identifier><identifier>DOI: 10.1111/j.1346-8138.1999.tb03519.x</identifier><identifier>PMID: 10091480</identifier><language>eng</language><publisher>England</publisher><subject>Adolescent ; Antigens, CD - analysis ; Basement Membrane - chemistry ; Blister - metabolism ; Cell Adhesion Molecules - analysis ; Collagen - analysis ; dystrophic epidermolysis bullosa ; Epidermolysis Bullosa Dystrophica - metabolism ; Humans ; Integrin alphaV ; Kalinin ; laminin 5 ; Male ; Skin - chemistry ; type IV collagen ; type VII collagen ; α6 integrin</subject><ispartof>Journal of dermatology, 1999-02, Vol.26 (2), p.106-110</ispartof><rights>1999 Japanese Dermatological Association</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3169-2211e9f76c1be2f8db1d5a21fafb071dc52adfb29894ca82023755f20e15cade3</citedby><cites>FETCH-LOGICAL-c3169-2211e9f76c1be2f8db1d5a21fafb071dc52adfb29894ca82023755f20e15cade3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1346-8138.1999.tb03519.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1346-8138.1999.tb03519.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27911,27912,45561,45562</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10091480$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Muramatsu, Tsutomu</creatorcontrib><creatorcontrib>Ko, Toshiko</creatorcontrib><creatorcontrib>Honoki, Kumiko</creatorcontrib><creatorcontrib>Hatoko, Mituso</creatorcontrib><creatorcontrib>Shirai, Toshihiko</creatorcontrib><creatorcontrib>Vnittanakom, Pramote</creatorcontrib><title>Intraepidermal Expression of Basement Membrane Components in the Lesional Skin of a Patient with Dystrophic Epidermolysis Bullosa</title><title>Journal of dermatology</title><addtitle>J Dermatol</addtitle><description>The patient was a 15‐year‐old male. Since birth, he had developed blistering and erosion of the skin. Biopsy skin specimen of the bullous lesions showed subepidermal blister formation. Electron microscopic examination revealed that tissue separation had occurred at the sublamina densa level. By indirect immunofluorescence using antibodies specific for α6 integrin, laminin 5, type IV collagen, and type VII collagen, all of these basement membrane components were detected as coarse granular intracytoplasmic deposits only in the basal and suprabasal cells of the blister roof. In the non‐blistered regions, these basement membrane components showed a linear pattern similar to that seen in normal skin. These findings suggest that intraepidermal expression of basement membrane components was closely related to the blister formation. The biological meaning of intraepidermal expression of basement membrane components were also discussed.</description><subject>Adolescent</subject><subject>Antigens, CD - analysis</subject><subject>Basement Membrane - chemistry</subject><subject>Blister - metabolism</subject><subject>Cell Adhesion Molecules - analysis</subject><subject>Collagen - analysis</subject><subject>dystrophic epidermolysis bullosa</subject><subject>Epidermolysis Bullosa Dystrophica - metabolism</subject><subject>Humans</subject><subject>Integrin alphaV</subject><subject>Kalinin</subject><subject>laminin 5</subject><subject>Male</subject><subject>Skin - chemistry</subject><subject>type IV collagen</subject><subject>type VII collagen</subject><subject>α6 integrin</subject><issn>0385-2407</issn><issn>1346-8138</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkcFP2zAUxq1p0-hg_8Jkcdgtwc-p03gnoHSsqGhIwNlykmfVXRIHOxXtcf85SVNNXOeLreff957e9xFyDiyG_lxsYkimaZRBksUgpYy7nCUCZLz7QCb_vj6SCUsyEfEpm52QLyFsGONSAPtMToAxCdOMTcjfZdN5ja0t0de6ootd6zEE6xrqDL3WAWtsOnqPde51g3Tu6tY1fSlQ29BujXSFA91LH__Yg0jTB93ZQfVquzW92YfOu3ZtC7oYx7hqH2yg19uqckGfkU9GVwG_Hu9T8vxz8TT_Fa1-3y7nV6uoSCCVEecAKM0sLSBHbrIyh1JoDkabnM2gLATXpcm5zOS00BlnPJkJYThDEIUuMTkl38e-rXcvWwydqm0osKr6tdw2qFSmgnHBe_DHCBbeheDRqNbbWvu9AqaGANRGDS6rwWU1BKCOAahdL_52nLLNayzfSUfHe-ByBF5thfv_aK3ubhaHZ_IGJVWZNg</recordid><startdate>199902</startdate><enddate>199902</enddate><creator>Muramatsu, Tsutomu</creator><creator>Ko, Toshiko</creator><creator>Honoki, Kumiko</creator><creator>Hatoko, Mituso</creator><creator>Shirai, Toshihiko</creator><creator>Vnittanakom, Pramote</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199902</creationdate><title>Intraepidermal Expression of Basement Membrane Components in the Lesional Skin of a Patient with Dystrophic Epidermolysis Bullosa</title><author>Muramatsu, Tsutomu ; Ko, Toshiko ; Honoki, Kumiko ; Hatoko, Mituso ; Shirai, Toshihiko ; Vnittanakom, Pramote</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3169-2211e9f76c1be2f8db1d5a21fafb071dc52adfb29894ca82023755f20e15cade3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adolescent</topic><topic>Antigens, CD - analysis</topic><topic>Basement Membrane - chemistry</topic><topic>Blister - metabolism</topic><topic>Cell Adhesion Molecules - analysis</topic><topic>Collagen - analysis</topic><topic>dystrophic epidermolysis bullosa</topic><topic>Epidermolysis Bullosa Dystrophica - metabolism</topic><topic>Humans</topic><topic>Integrin alphaV</topic><topic>Kalinin</topic><topic>laminin 5</topic><topic>Male</topic><topic>Skin - chemistry</topic><topic>type IV collagen</topic><topic>type VII collagen</topic><topic>α6 integrin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Muramatsu, Tsutomu</creatorcontrib><creatorcontrib>Ko, Toshiko</creatorcontrib><creatorcontrib>Honoki, Kumiko</creatorcontrib><creatorcontrib>Hatoko, Mituso</creatorcontrib><creatorcontrib>Shirai, Toshihiko</creatorcontrib><creatorcontrib>Vnittanakom, Pramote</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Muramatsu, Tsutomu</au><au>Ko, Toshiko</au><au>Honoki, Kumiko</au><au>Hatoko, Mituso</au><au>Shirai, Toshihiko</au><au>Vnittanakom, Pramote</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intraepidermal Expression of Basement Membrane Components in the Lesional Skin of a Patient with Dystrophic Epidermolysis Bullosa</atitle><jtitle>Journal of dermatology</jtitle><addtitle>J Dermatol</addtitle><date>1999-02</date><risdate>1999</risdate><volume>26</volume><issue>2</issue><spage>106</spage><epage>110</epage><pages>106-110</pages><issn>0385-2407</issn><eissn>1346-8138</eissn><abstract>The patient was a 15‐year‐old male. Since birth, he had developed blistering and erosion of the skin. Biopsy skin specimen of the bullous lesions showed subepidermal blister formation. Electron microscopic examination revealed that tissue separation had occurred at the sublamina densa level. By indirect immunofluorescence using antibodies specific for α6 integrin, laminin 5, type IV collagen, and type VII collagen, all of these basement membrane components were detected as coarse granular intracytoplasmic deposits only in the basal and suprabasal cells of the blister roof. In the non‐blistered regions, these basement membrane components showed a linear pattern similar to that seen in normal skin. These findings suggest that intraepidermal expression of basement membrane components was closely related to the blister formation. The biological meaning of intraepidermal expression of basement membrane components were also discussed.</abstract><cop>England</cop><pmid>10091480</pmid><doi>10.1111/j.1346-8138.1999.tb03519.x</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0385-2407 |
| ispartof | Journal of dermatology, 1999-02, Vol.26 (2), p.106-110 |
| issn | 0385-2407 1346-8138 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Adolescent Antigens, CD - analysis Basement Membrane - chemistry Blister - metabolism Cell Adhesion Molecules - analysis Collagen - analysis dystrophic epidermolysis bullosa Epidermolysis Bullosa Dystrophica - metabolism Humans Integrin alphaV Kalinin laminin 5 Male Skin - chemistry type IV collagen type VII collagen α6 integrin |
| title | Intraepidermal Expression of Basement Membrane Components in the Lesional Skin of a Patient with Dystrophic Epidermolysis Bullosa |
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