Cloning and expression analyses of sucrose non-fermenting-1-related kinase 1 (SnRK1b) gene during development of sorghum and maize endosperm and its implicated role in sugar-to-starch metabolic transition
A full-length cDNA clone, SbSnRK1b (1530 bp, GenBank accession no. EF544393), encoding a putative serine/threonine protein kinase homologue of yeast (Saccharomyces cerevisiae) SNF1, was isolated from developing endosperm of sorghum [Sorghum bicolor (L.) Moench]. Multiple sequence alignment data show...
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| description | A full-length cDNA clone, SbSnRK1b (1530 bp, GenBank accession no. EF544393), encoding a putative serine/threonine protein kinase homologue of yeast (Saccharomyces cerevisiae) SNF1, was isolated from developing endosperm of sorghum [Sorghum bicolor (L.) Moench]. Multiple sequence alignment data showed a phylogenetic affiliation of the sorghum clone with the SnRK1b group of protein kinases that are highly expressed in cereal seed endosperm. The DNA gel blot analyses indicated that SbSnRK1b gene is present as a single- or low copy number gene in sorghum. The RNA and protein gel blot analyses confirmed the expression of SbSnRK1b in developing sorghum caryopses, overlapping with the starch biosynthesis phase, 12-24 days after fertilization. In situ hybridization and immunolocalization data resolved the spatial specificity of SbSnRK1b expression in the basal endosperm transfer cell layer, the unique port of assimilate unloading in the growing sorghum seed. Expression of SbSnRK1b was also evident in the developing sorghum microspores, coincident with the onset of starch deposition phase. As in sorghum, similar spatiotemporal specificity of SnRK1b expression was observed during maize (Zea mays L.) seed development. However, discordant in situ hybridization and immunolocalization data indicated that the expression of SbSnRK1b homologue in maize is under posttranscriptional control during endosperm development. |
| doi_str_mv | 10.1111/j.1399-3054.2008.01106.x |
| format | Article |
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EF544393), encoding a putative serine/threonine protein kinase homologue of yeast (Saccharomyces cerevisiae) SNF1, was isolated from developing endosperm of sorghum [Sorghum bicolor (L.) Moench]. Multiple sequence alignment data showed a phylogenetic affiliation of the sorghum clone with the SnRK1b group of protein kinases that are highly expressed in cereal seed endosperm. The DNA gel blot analyses indicated that SbSnRK1b gene is present as a single- or low copy number gene in sorghum. The RNA and protein gel blot analyses confirmed the expression of SbSnRK1b in developing sorghum caryopses, overlapping with the starch biosynthesis phase, 12-24 days after fertilization. In situ hybridization and immunolocalization data resolved the spatial specificity of SbSnRK1b expression in the basal endosperm transfer cell layer, the unique port of assimilate unloading in the growing sorghum seed. Expression of SbSnRK1b was also evident in the developing sorghum microspores, coincident with the onset of starch deposition phase. As in sorghum, similar spatiotemporal specificity of SnRK1b expression was observed during maize (Zea mays L.) seed development. However, discordant in situ hybridization and immunolocalization data indicated that the expression of SbSnRK1b homologue in maize is under posttranscriptional control during endosperm development.</description><identifier>ISSN: 0031-9317</identifier><identifier>EISSN: 1399-3054</identifier><identifier>DOI: 10.1111/j.1399-3054.2008.01106.x</identifier><identifier>PMID: 18433416</identifier><identifier>CODEN: PHPLAI</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Amino Acid Sequence ; Biological and medical sciences ; Blotting, Northern ; Blotting, Southern ; Blotting, Western ; Carbohydrate Metabolism ; Cloning, Molecular ; Electrophoresis, Polyacrylamide Gel ; Fundamental and applied biological sciences. Psychology ; Gene Expression Profiling ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Plant ; In Situ Hybridization ; Metabolism ; Molecular Sequence Data ; Photosynthesis, respiration. Anabolism, catabolism ; Phylogeny ; Plant physiology and development ; Plant Proteins - classification ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Protein-Serine-Threonine Kinases - genetics ; Protein-Serine-Threonine Kinases - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Seeds - genetics ; Seeds - growth & development ; Seeds - metabolism ; Sequence Alignment ; Sorghum ; Sorghum - genetics ; Sorghum - growth & development ; Sorghum - metabolism ; Starch - biosynthesis ; Zea mays ; Zea mays - genetics ; Zea mays - growth & development ; Zea mays - metabolism</subject><ispartof>Physiologia plantarum, 2008-09, Vol.134 (1), p.161-173</ispartof><rights>Physiologia Plantarum 2008</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4906-ff006a9bc7d3d406ab9c577641a87e3e206db97eaadcd1a99b239148ffe0a68a3</citedby><cites>FETCH-LOGICAL-c4906-ff006a9bc7d3d406ab9c577641a87e3e206db97eaadcd1a99b239148ffe0a68a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1399-3054.2008.01106.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1399-3054.2008.01106.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20605009$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18433416$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jain, Mukesh</creatorcontrib><creatorcontrib>Li, Qin-Bao</creatorcontrib><creatorcontrib>Chourey, Prem S</creatorcontrib><title>Cloning and expression analyses of sucrose non-fermenting-1-related kinase 1 (SnRK1b) gene during development of sorghum and maize endosperm and its implicated role in sugar-to-starch metabolic transition</title><title>Physiologia plantarum</title><addtitle>Physiol Plant</addtitle><description>A full-length cDNA clone, SbSnRK1b (1530 bp, GenBank accession no. EF544393), encoding a putative serine/threonine protein kinase homologue of yeast (Saccharomyces cerevisiae) SNF1, was isolated from developing endosperm of sorghum [Sorghum bicolor (L.) Moench]. Multiple sequence alignment data showed a phylogenetic affiliation of the sorghum clone with the SnRK1b group of protein kinases that are highly expressed in cereal seed endosperm. The DNA gel blot analyses indicated that SbSnRK1b gene is present as a single- or low copy number gene in sorghum. The RNA and protein gel blot analyses confirmed the expression of SbSnRK1b in developing sorghum caryopses, overlapping with the starch biosynthesis phase, 12-24 days after fertilization. In situ hybridization and immunolocalization data resolved the spatial specificity of SbSnRK1b expression in the basal endosperm transfer cell layer, the unique port of assimilate unloading in the growing sorghum seed. Expression of SbSnRK1b was also evident in the developing sorghum microspores, coincident with the onset of starch deposition phase. As in sorghum, similar spatiotemporal specificity of SnRK1b expression was observed during maize (Zea mays L.) seed development. However, discordant in situ hybridization and immunolocalization data indicated that the expression of SbSnRK1b homologue in maize is under posttranscriptional control during endosperm development.</description><subject>Amino Acid Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Blotting, Southern</subject><subject>Blotting, Western</subject><subject>Carbohydrate Metabolism</subject><subject>Cloning, Molecular</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene Expression Regulation, Plant</subject><subject>In Situ Hybridization</subject><subject>Metabolism</subject><subject>Molecular Sequence Data</subject><subject>Photosynthesis, respiration. Anabolism, catabolism</subject><subject>Phylogeny</subject><subject>Plant physiology and development</subject><subject>Plant Proteins - classification</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Protein-Serine-Threonine Kinases - genetics</subject><subject>Protein-Serine-Threonine Kinases - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Seeds - genetics</subject><subject>Seeds - growth & development</subject><subject>Seeds - metabolism</subject><subject>Sequence Alignment</subject><subject>Sorghum</subject><subject>Sorghum - genetics</subject><subject>Sorghum - growth & development</subject><subject>Sorghum - metabolism</subject><subject>Starch - biosynthesis</subject><subject>Zea mays</subject><subject>Zea mays - genetics</subject><subject>Zea mays - growth & development</subject><subject>Zea mays - metabolism</subject><issn>0031-9317</issn><issn>1399-3054</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcFy0zAURT0MDA2FXwBtYGDhIFmybC-6gAwUphnoUEqXmmf7OVVqS0ayIeEb-SjkJBOW4I0l69z7rnWjiDA6Z-F5vZ4zXhQxp6mYJ5Tmc8oYlfPNvWh2PLgfzSjlLC44y06iR96vKWVSsuRhdMJywblgchb9XrTWaLMiYGqCm96h99qasIV269ET2xA_Vs56JMaauEHXoRmCImaxwxYGrMmdNhDOGXl5Zb5csPIVWaFBUo9ucq7xB7a2n2Q7N-tWt2O3G9iB_oUETW19H4x33_Tgie76Vlc7b2dbJNqEECtw8WBjP4CrbkmHA5Q2UGRwYLweQurH0YMGWo9PDu_T6Pr9u6-LD_Hy8_nHxZtlXImCyrhpKJVQlFVW81qEZVlUaZZJwSDPkGNCZV0WGQLUVc2gKMqEF0zkTYMUZA78NHqx9-2d_T6iH1SnfYVtCwbt6JUspMgzIf4JJjQTlOVFAPM9ON20d9io3ukO3FYxqqbK1VpNzaqpWTVVrnaVq02QPj3MGMsO67_CQ8cBeH4AwFfQNuG6Ku2PXPhbmlI6ZTjbcz91i9v_DqAuL5fTKujjvV77ATdHPbg7JTOeperm07lK3qbfLtL0Rk3znu35BqyClQuZrq8SyngIk4iUU_4HGTvjwg</recordid><startdate>200809</startdate><enddate>200809</enddate><creator>Jain, Mukesh</creator><creator>Li, Qin-Bao</creator><creator>Chourey, Prem S</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200809</creationdate><title>Cloning and expression analyses of sucrose non-fermenting-1-related kinase 1 (SnRK1b) gene during development of sorghum and maize endosperm and its implicated role in sugar-to-starch metabolic transition</title><author>Jain, Mukesh ; Li, Qin-Bao ; Chourey, Prem S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4906-ff006a9bc7d3d406ab9c577641a87e3e206db97eaadcd1a99b239148ffe0a68a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Amino Acid Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Blotting, Southern</topic><topic>Blotting, Western</topic><topic>Carbohydrate Metabolism</topic><topic>Cloning, Molecular</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene Expression Regulation, Plant</topic><topic>In Situ Hybridization</topic><topic>Metabolism</topic><topic>Molecular Sequence Data</topic><topic>Photosynthesis, respiration. Anabolism, catabolism</topic><topic>Phylogeny</topic><topic>Plant physiology and development</topic><topic>Plant Proteins - classification</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Protein-Serine-Threonine Kinases - genetics</topic><topic>Protein-Serine-Threonine Kinases - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Seeds - genetics</topic><topic>Seeds - growth & development</topic><topic>Seeds - metabolism</topic><topic>Sequence Alignment</topic><topic>Sorghum</topic><topic>Sorghum - genetics</topic><topic>Sorghum - growth & development</topic><topic>Sorghum - metabolism</topic><topic>Starch - biosynthesis</topic><topic>Zea mays</topic><topic>Zea mays - genetics</topic><topic>Zea mays - growth & development</topic><topic>Zea mays - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jain, Mukesh</creatorcontrib><creatorcontrib>Li, Qin-Bao</creatorcontrib><creatorcontrib>Chourey, Prem S</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Physiologia plantarum</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jain, Mukesh</au><au>Li, Qin-Bao</au><au>Chourey, Prem S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and expression analyses of sucrose non-fermenting-1-related kinase 1 (SnRK1b) gene during development of sorghum and maize endosperm and its implicated role in sugar-to-starch metabolic transition</atitle><jtitle>Physiologia plantarum</jtitle><addtitle>Physiol Plant</addtitle><date>2008-09</date><risdate>2008</risdate><volume>134</volume><issue>1</issue><spage>161</spage><epage>173</epage><pages>161-173</pages><issn>0031-9317</issn><eissn>1399-3054</eissn><coden>PHPLAI</coden><abstract>A full-length cDNA clone, SbSnRK1b (1530 bp, GenBank accession no. EF544393), encoding a putative serine/threonine protein kinase homologue of yeast (Saccharomyces cerevisiae) SNF1, was isolated from developing endosperm of sorghum [Sorghum bicolor (L.) Moench]. Multiple sequence alignment data showed a phylogenetic affiliation of the sorghum clone with the SnRK1b group of protein kinases that are highly expressed in cereal seed endosperm. The DNA gel blot analyses indicated that SbSnRK1b gene is present as a single- or low copy number gene in sorghum. The RNA and protein gel blot analyses confirmed the expression of SbSnRK1b in developing sorghum caryopses, overlapping with the starch biosynthesis phase, 12-24 days after fertilization. In situ hybridization and immunolocalization data resolved the spatial specificity of SbSnRK1b expression in the basal endosperm transfer cell layer, the unique port of assimilate unloading in the growing sorghum seed. Expression of SbSnRK1b was also evident in the developing sorghum microspores, coincident with the onset of starch deposition phase. As in sorghum, similar spatiotemporal specificity of SnRK1b expression was observed during maize (Zea mays L.) seed development. However, discordant in situ hybridization and immunolocalization data indicated that the expression of SbSnRK1b homologue in maize is under posttranscriptional control during endosperm development.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>18433416</pmid><doi>10.1111/j.1399-3054.2008.01106.x</doi><tpages>13</tpages></addata></record> |
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| subjects | Amino Acid Sequence Biological and medical sciences Blotting, Northern Blotting, Southern Blotting, Western Carbohydrate Metabolism Cloning, Molecular Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Gene Expression Profiling Gene Expression Regulation, Developmental Gene Expression Regulation, Plant In Situ Hybridization Metabolism Molecular Sequence Data Photosynthesis, respiration. Anabolism, catabolism Phylogeny Plant physiology and development Plant Proteins - classification Plant Proteins - genetics Plant Proteins - metabolism Protein-Serine-Threonine Kinases - genetics Protein-Serine-Threonine Kinases - metabolism Reverse Transcriptase Polymerase Chain Reaction Seeds - genetics Seeds - growth & development Seeds - metabolism Sequence Alignment Sorghum Sorghum - genetics Sorghum - growth & development Sorghum - metabolism Starch - biosynthesis Zea mays Zea mays - genetics Zea mays - growth & development Zea mays - metabolism |
| title | Cloning and expression analyses of sucrose non-fermenting-1-related kinase 1 (SnRK1b) gene during development of sorghum and maize endosperm and its implicated role in sugar-to-starch metabolic transition |
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