Identification of genes specifically expressed in cauliflower reproductive meristems; Molecular characterization of BoREM1
Using the meristems of the cauliflower curd as a source of tissue and a series of subtractive hybridizations and amplification reactions, we have constructed a cDNA library highly enriched in cDNAs expressed in reproductive meristems. The analysis of a sample of 250 clones from this library identifi...
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| Veröffentlicht in: | Plant molecular biology 1999-02, Vol.39 (3), p.427-436 |
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| creator | Franco-Zorrilla, J.M. (Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria, Madrid (Spain). Dept. de Mejora Genetica y Biotecnologia) Fernandez-Calvin, B Madueno, E Cruz-Alvarez, M Salinas, J Martinez-Zapater, J.M |
| description | Using the meristems of the cauliflower curd as a source of tissue and a series of subtractive hybridizations and amplification reactions, we have constructed a cDNA library highly enriched in cDNAs expressed in reproductive meristems. The analysis of a sample of 250 clones from this library identified 22 cDNA clones corresponding to genes specifically expressed in these cauliflower meristems. Apart from two clones that corresponded to APETALA1, and two other ones showing similarity to different aminoacyl-tRNA synthetases, the remaining clones showed no similarity to any sequence in the databases and may correspond to novel genes. One of these clones, BoREM1, was further characterized and found to correspond to a gene encoding a protein with features of regulatory proteins that follows a expression pattern very similar to the LEAFY transcripts. |
| doi_str_mv | 10.1023/A:1006130629100 |
| format | Article |
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(Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria, Madrid (Spain). Dept. de Mejora Genetica y Biotecnologia) ; Fernandez-Calvin, B ; Madueno, E ; Cruz-Alvarez, M ; Salinas, J ; Martinez-Zapater, J.M</creator><creatorcontrib>Franco-Zorrilla, J.M. (Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria, Madrid (Spain). Dept. de Mejora Genetica y Biotecnologia) ; Fernandez-Calvin, B ; Madueno, E ; Cruz-Alvarez, M ; Salinas, J ; Martinez-Zapater, J.M</creatorcontrib><description>Using the meristems of the cauliflower curd as a source of tissue and a series of subtractive hybridizations and amplification reactions, we have constructed a cDNA library highly enriched in cDNAs expressed in reproductive meristems. The analysis of a sample of 250 clones from this library identified 22 cDNA clones corresponding to genes specifically expressed in these cauliflower meristems. Apart from two clones that corresponded to APETALA1, and two other ones showing similarity to different aminoacyl-tRNA synthetases, the remaining clones showed no similarity to any sequence in the databases and may correspond to novel genes. One of these clones, BoREM1, was further characterized and found to correspond to a gene encoding a protein with features of regulatory proteins that follows a expression pattern very similar to the LEAFY transcripts.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1023/A:1006130629100</identifier><identifier>PMID: 10092172</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Amino Acid Sequence ; Base Sequence ; Brassica - chemistry ; Brassica - genetics ; BRASSICA OLERACEA BOTRYTIS ; DNA, Complementary - chemistry ; DNA, Complementary - genetics ; EXPRESION GENICA ; EXPRESSION DES GENES ; GENE EXPRESSION ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Plant ; Genes ; Genes, Plant - genetics ; Meristem - metabolism ; MERISTEMAS ; MERISTEME ; MERISTEMS ; Molecular Sequence Data ; Plant Proteins - genetics ; RNA, Plant - genetics ; RNA, Plant - metabolism ; Sequence Analysis, DNA ; Tissue Distribution ; Transcription, Genetic</subject><ispartof>Plant molecular biology, 1999-02, Vol.39 (3), p.427-436</ispartof><rights>Kluwer Academic Publishers 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c374t-7ca4c5d2d0c2f270c6ddaebc7fe7a68c5c683b79d1fde0372d3a6c3db98ff6913</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10092172$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Franco-Zorrilla, J.M. 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One of these clones, BoREM1, was further characterized and found to correspond to a gene encoding a protein with features of regulatory proteins that follows a expression pattern very similar to the LEAFY transcripts.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Brassica - chemistry</subject><subject>Brassica - genetics</subject><subject>BRASSICA OLERACEA BOTRYTIS</subject><subject>DNA, Complementary - chemistry</subject><subject>DNA, Complementary - genetics</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>GENE EXPRESSION</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Genes, Plant - genetics</subject><subject>Meristem - metabolism</subject><subject>MERISTEMAS</subject><subject>MERISTEME</subject><subject>MERISTEMS</subject><subject>Molecular Sequence Data</subject><subject>Plant Proteins - genetics</subject><subject>RNA, Plant - genetics</subject><subject>RNA, Plant - metabolism</subject><subject>Sequence Analysis, DNA</subject><subject>Tissue Distribution</subject><subject>Transcription, Genetic</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkc1PFjEQxhuigRfkzEnSePC20o_ddqsnJKgkL5gYPW_6TqdQ0t2-trsq_PU0gh68eHomM7_MxzOEHHH2hjMhT07fcsYUl0wJU6MdsuKdlk3HRP-MrBhXumlbLvbIfim3jFVEql2yV9UIrsWK3F84nObgA9g5pIkmT69xwkLLFuF3OsY7ir-2GUtBR8NEwS4x-Jh-YqYZtzm5BebwA-mIOZQZx_KOXqaIsESbKdzYbGGupfu_E96nL-eX_AV57m0sePikB-Tbh_OvZ5-a9eePF2en6wakbudGg22hc8IxEF5oBso5ixvQHrVVPXSgernRxnHvkEktnLQKpNuY3ntluDwgrx_71lW_L1jmYQwFMEY7YVrKoIxqle7lf8FqGJe9YBV89Q94m5Y81SMGrXnbSa7bCh0_QctmRDdscxhtvhv-eF-Bl4-At2mw19W74WrNjTH1SUIq-QBunpE1</recordid><startdate>199902</startdate><enddate>199902</enddate><creator>Franco-Zorrilla, J.M. 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The analysis of a sample of 250 clones from this library identified 22 cDNA clones corresponding to genes specifically expressed in these cauliflower meristems. Apart from two clones that corresponded to APETALA1, and two other ones showing similarity to different aminoacyl-tRNA synthetases, the remaining clones showed no similarity to any sequence in the databases and may correspond to novel genes. One of these clones, BoREM1, was further characterized and found to correspond to a gene encoding a protein with features of regulatory proteins that follows a expression pattern very similar to the LEAFY transcripts.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>10092172</pmid><doi>10.1023/A:1006130629100</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0167-4412 |
| ispartof | Plant molecular biology, 1999-02, Vol.39 (3), p.427-436 |
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| language | eng |
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| subjects | Amino Acid Sequence Base Sequence Brassica - chemistry Brassica - genetics BRASSICA OLERACEA BOTRYTIS DNA, Complementary - chemistry DNA, Complementary - genetics EXPRESION GENICA EXPRESSION DES GENES GENE EXPRESSION Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Genes Genes, Plant - genetics Meristem - metabolism MERISTEMAS MERISTEME MERISTEMS Molecular Sequence Data Plant Proteins - genetics RNA, Plant - genetics RNA, Plant - metabolism Sequence Analysis, DNA Tissue Distribution Transcription, Genetic |
| title | Identification of genes specifically expressed in cauliflower reproductive meristems; Molecular characterization of BoREM1 |
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