Generation of infectious genome of bovine adenovirus type 3 by homologous recombination in bacteria

The widely used technique of generating adenovirus vectors by homologous recombination in mammalian cells is usually not very efficient. This communication describes a simple method of generating a plasmid containing the full-length genome of an adenovirus by homologous recombination in bacteria. Fo...

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Veröffentlicht in:	Journal of virological methods 1999-02, Vol.77 (2), p.125-129
Hauptverfasser:	van Olphen, Alberto L., Mittal, Suresh K.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenovirus Adenovirus vector Animals Biological and medical sciences Biotechnology Bovine adenovirus Bovine adenovirus 3 Cattle Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Gene therapy Genetic engineering Genetic technics Genetics Genome, Viral Mastadenovirus - genetics Mastadenovirus - pathogenicity Methods. Procedures. Technologies Microbiology Plasmids Recombination in E. coli Recombination, Genetic Vectors (cloning, transfer, expression). Insertion sequences and transposons Virology
Online-Zugang:	Volltext
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