Genetic diversity of equine arteritis virus
T Stadejek, H Bjorklund, CR Bascunana, IM Ciabatti, MT Scicluna, D Amaddeo, WH McCollum, GL Autorino, PJ Timoney, DJ Paton, B Klingeborn and S Belak Department of Virology, The National Veterinary Institute, Biomedical Center, Uppsala, Sweden. Equine arteritis viruses (EAV) from Europe and America w...
Gespeichert in:
| Veröffentlicht in: | Journal of general virology 1999-03, Vol.80 (3), p.691-699 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 699 |
|---|---|
| container_issue | 3 |
| container_start_page | 691 |
| container_title | Journal of general virology |
| container_volume | 80 |
| creator | Stadejek, T Bjorklund, H Bascunana, CR Ciabatti, IM Scicluna, MT Amaddeo, D McCollum, WH Autorino, GL Timoney, PJ Paton, DJ Klingeborn, B Belak, S |
| description | T Stadejek, H Bjorklund, CR Bascunana, IM Ciabatti, MT Scicluna, D Amaddeo, WH McCollum, GL Autorino, PJ Timoney, DJ Paton, B Klingeborn and S Belak
Department of Virology, The National Veterinary Institute, Biomedical Center, Uppsala, Sweden.
Equine arteritis viruses (EAV) from Europe and America were compared by
phylogenetic analysis of 43 isolates obtained over four decades. An
additional 22 virus sequences were retrieved from GenBank. Fragments of the
glycoprotein G(L) and the replicase genes were amplified by RT-PCR, prior
to sequencing and construction of phylogenetic trees. The trees revealed
many distinctive lineages, consistent with prolonged diversification within
geographically separated host populations. Two large groups and five
subgroups were distinguished. Group I consisted mainly of viruses from
North America, whilst group II consisted mainly of European isolates. In
most instances, where the geographic origin of the viruses appeared to be
at variance with the phylogenetically predicted relationships, the horses
from which the viruses were recovered had been transported between Europe
and America or vice versa. Analysis of the replicase gene revealed similar
phylogenetic relationships although not all of the groups were as clearly
defined. Virus strains CH1 (Switzerland, 1964) and S1 (Sweden, 1989)
represented separate 'outgroups' based on analysis of both genomic regions.
The results of this study confirm the value of the G(L) gene of EAV for
estimating virus genetic diversity and as a useful tool for tracing routes
by which EAV is spread. In addition, computer-assisted predictions of
antigenic sites on the G(L) protein revealed considerable variability among
the isolates, especially with respect to regions associated with
neutralization domains. |
| doi_str_mv | 10.1099/0022-1317-80-3-691 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69645823</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69645823</sourcerecordid><originalsourceid>FETCH-LOGICAL-c406t-7e8f3ed666c5662b86fc53bb9740dded1c28b47e4f9633656dcd8b42809d17d13</originalsourceid><addsrcrecordid>eNqFkE9LwzAYh4Mobk6_gAfpSQ8Szb-mzVGGTmHgRc-hTd5ukbXdknayb29KZXjz8BJ48-T3Cw9C15Q8UKLUIyGMYcpphnOCOZaKnqApFTLFLF6foukRmKCLEL4IoUKk2TmaUEIUizNF9wtooHMmsW4PPrjukLRVArveNZAUvgPvOheSvfN9uERnVbEJcPV7ztDny_PH_BUv3xdv86clNoLIDmeQVxyslNKkUrIyl5VJeVmqTBBrwVLD8lJkIColOZeptMbGBcuJsjSzlM_Q7Zi79e2uh9Dp2gUDm03RQNsHLZUUac74vyDNmKQ8JRFkI2h8G4KHSm-9qwt_0JTowaUeVOlBlc6J5rFi-MbNb3pf1mD_PBnlReBuBNZutf52HvQKmtrFjtK1Oio7Rv0Aa6l7qQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17261350</pqid></control><display><type>article</type><title>Genetic diversity of equine arteritis virus</title><source>MEDLINE</source><source>Microbiology Society</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Stadejek, T ; Bjorklund, H ; Bascunana, CR ; Ciabatti, IM ; Scicluna, MT ; Amaddeo, D ; McCollum, WH ; Autorino, GL ; Timoney, PJ ; Paton, DJ ; Klingeborn, B ; Belak, S</creator><creatorcontrib>Stadejek, T ; Bjorklund, H ; Bascunana, CR ; Ciabatti, IM ; Scicluna, MT ; Amaddeo, D ; McCollum, WH ; Autorino, GL ; Timoney, PJ ; Paton, DJ ; Klingeborn, B ; Belak, S</creatorcontrib><description>T Stadejek, H Bjorklund, CR Bascunana, IM Ciabatti, MT Scicluna, D Amaddeo, WH McCollum, GL Autorino, PJ Timoney, DJ Paton, B Klingeborn and S Belak
Department of Virology, The National Veterinary Institute, Biomedical Center, Uppsala, Sweden.
Equine arteritis viruses (EAV) from Europe and America were compared by
phylogenetic analysis of 43 isolates obtained over four decades. An
additional 22 virus sequences were retrieved from GenBank. Fragments of the
glycoprotein G(L) and the replicase genes were amplified by RT-PCR, prior
to sequencing and construction of phylogenetic trees. The trees revealed
many distinctive lineages, consistent with prolonged diversification within
geographically separated host populations. Two large groups and five
subgroups were distinguished. Group I consisted mainly of viruses from
North America, whilst group II consisted mainly of European isolates. In
most instances, where the geographic origin of the viruses appeared to be
at variance with the phylogenetically predicted relationships, the horses
from which the viruses were recovered had been transported between Europe
and America or vice versa. Analysis of the replicase gene revealed similar
phylogenetic relationships although not all of the groups were as clearly
defined. Virus strains CH1 (Switzerland, 1964) and S1 (Sweden, 1989)
represented separate 'outgroups' based on analysis of both genomic regions.
The results of this study confirm the value of the G(L) gene of EAV for
estimating virus genetic diversity and as a useful tool for tracing routes
by which EAV is spread. In addition, computer-assisted predictions of
antigenic sites on the G(L) protein revealed considerable variability among
the isolates, especially with respect to regions associated with
neutralization domains.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-80-3-691</identifier><identifier>PMID: 10092009</identifier><language>eng</language><publisher>England: Soc General Microbiol</publisher><subject>Amino Acid Sequence ; Animals ; Antibodies, Viral - immunology ; Antigens, Viral - chemistry ; Antigens, Viral - genetics ; Antigens, Viral - immunology ; Arterivirus Infections - immunology ; Arterivirus Infections - transmission ; Arterivirus Infections - veterinary ; Arterivirus Infections - virology ; Cell Line ; Equartevirus - classification ; Equartevirus - genetics ; Equartevirus - immunology ; Equine arteritis virus ; Europe ; Genes, Viral - genetics ; Genetic Variation - genetics ; Genome, Viral ; Glycoproteins - chemistry ; Glycoproteins - genetics ; Glycoproteins - immunology ; Horses - virology ; Immunodominant Epitopes - chemistry ; Immunodominant Epitopes - genetics ; Immunodominant Epitopes - immunology ; Molecular Sequence Data ; North America ; Phylogeny ; Reverse Transcriptase Polymerase Chain Reaction ; RNA Replicase - genetics ; Sequence Analysis, DNA ; Terminology as Topic</subject><ispartof>Journal of general virology, 1999-03, Vol.80 (3), p.691-699</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-7e8f3ed666c5662b86fc53bb9740dded1c28b47e4f9633656dcd8b42809d17d13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3737,3738,27915,27916</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10092009$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Stadejek, T</creatorcontrib><creatorcontrib>Bjorklund, H</creatorcontrib><creatorcontrib>Bascunana, CR</creatorcontrib><creatorcontrib>Ciabatti, IM</creatorcontrib><creatorcontrib>Scicluna, MT</creatorcontrib><creatorcontrib>Amaddeo, D</creatorcontrib><creatorcontrib>McCollum, WH</creatorcontrib><creatorcontrib>Autorino, GL</creatorcontrib><creatorcontrib>Timoney, PJ</creatorcontrib><creatorcontrib>Paton, DJ</creatorcontrib><creatorcontrib>Klingeborn, B</creatorcontrib><creatorcontrib>Belak, S</creatorcontrib><title>Genetic diversity of equine arteritis virus</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>T Stadejek, H Bjorklund, CR Bascunana, IM Ciabatti, MT Scicluna, D Amaddeo, WH McCollum, GL Autorino, PJ Timoney, DJ Paton, B Klingeborn and S Belak
Department of Virology, The National Veterinary Institute, Biomedical Center, Uppsala, Sweden.
Equine arteritis viruses (EAV) from Europe and America were compared by
phylogenetic analysis of 43 isolates obtained over four decades. An
additional 22 virus sequences were retrieved from GenBank. Fragments of the
glycoprotein G(L) and the replicase genes were amplified by RT-PCR, prior
to sequencing and construction of phylogenetic trees. The trees revealed
many distinctive lineages, consistent with prolonged diversification within
geographically separated host populations. Two large groups and five
subgroups were distinguished. Group I consisted mainly of viruses from
North America, whilst group II consisted mainly of European isolates. In
most instances, where the geographic origin of the viruses appeared to be
at variance with the phylogenetically predicted relationships, the horses
from which the viruses were recovered had been transported between Europe
and America or vice versa. Analysis of the replicase gene revealed similar
phylogenetic relationships although not all of the groups were as clearly
defined. Virus strains CH1 (Switzerland, 1964) and S1 (Sweden, 1989)
represented separate 'outgroups' based on analysis of both genomic regions.
The results of this study confirm the value of the G(L) gene of EAV for
estimating virus genetic diversity and as a useful tool for tracing routes
by which EAV is spread. In addition, computer-assisted predictions of
antigenic sites on the G(L) protein revealed considerable variability among
the isolates, especially with respect to regions associated with
neutralization domains.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antibodies, Viral - immunology</subject><subject>Antigens, Viral - chemistry</subject><subject>Antigens, Viral - genetics</subject><subject>Antigens, Viral - immunology</subject><subject>Arterivirus Infections - immunology</subject><subject>Arterivirus Infections - transmission</subject><subject>Arterivirus Infections - veterinary</subject><subject>Arterivirus Infections - virology</subject><subject>Cell Line</subject><subject>Equartevirus - classification</subject><subject>Equartevirus - genetics</subject><subject>Equartevirus - immunology</subject><subject>Equine arteritis virus</subject><subject>Europe</subject><subject>Genes, Viral - genetics</subject><subject>Genetic Variation - genetics</subject><subject>Genome, Viral</subject><subject>Glycoproteins - chemistry</subject><subject>Glycoproteins - genetics</subject><subject>Glycoproteins - immunology</subject><subject>Horses - virology</subject><subject>Immunodominant Epitopes - chemistry</subject><subject>Immunodominant Epitopes - genetics</subject><subject>Immunodominant Epitopes - immunology</subject><subject>Molecular Sequence Data</subject><subject>North America</subject><subject>Phylogeny</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA Replicase - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Terminology as Topic</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9LwzAYh4Mobk6_gAfpSQ8Szb-mzVGGTmHgRc-hTd5ukbXdknayb29KZXjz8BJ48-T3Cw9C15Q8UKLUIyGMYcpphnOCOZaKnqApFTLFLF6foukRmKCLEL4IoUKk2TmaUEIUizNF9wtooHMmsW4PPrjukLRVArveNZAUvgPvOheSvfN9uERnVbEJcPV7ztDny_PH_BUv3xdv86clNoLIDmeQVxyslNKkUrIyl5VJeVmqTBBrwVLD8lJkIColOZeptMbGBcuJsjSzlM_Q7Zi79e2uh9Dp2gUDm03RQNsHLZUUac74vyDNmKQ8JRFkI2h8G4KHSm-9qwt_0JTowaUeVOlBlc6J5rFi-MbNb3pf1mD_PBnlReBuBNZutf52HvQKmtrFjtK1Oio7Rv0Aa6l7qQ</recordid><startdate>19990301</startdate><enddate>19990301</enddate><creator>Stadejek, T</creator><creator>Bjorklund, H</creator><creator>Bascunana, CR</creator><creator>Ciabatti, IM</creator><creator>Scicluna, MT</creator><creator>Amaddeo, D</creator><creator>McCollum, WH</creator><creator>Autorino, GL</creator><creator>Timoney, PJ</creator><creator>Paton, DJ</creator><creator>Klingeborn, B</creator><creator>Belak, S</creator><general>Soc General Microbiol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19990301</creationdate><title>Genetic diversity of equine arteritis virus</title><author>Stadejek, T ; Bjorklund, H ; Bascunana, CR ; Ciabatti, IM ; Scicluna, MT ; Amaddeo, D ; McCollum, WH ; Autorino, GL ; Timoney, PJ ; Paton, DJ ; Klingeborn, B ; Belak, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-7e8f3ed666c5662b86fc53bb9740dded1c28b47e4f9633656dcd8b42809d17d13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antibodies, Viral - immunology</topic><topic>Antigens, Viral - chemistry</topic><topic>Antigens, Viral - genetics</topic><topic>Antigens, Viral - immunology</topic><topic>Arterivirus Infections - immunology</topic><topic>Arterivirus Infections - transmission</topic><topic>Arterivirus Infections - veterinary</topic><topic>Arterivirus Infections - virology</topic><topic>Cell Line</topic><topic>Equartevirus - classification</topic><topic>Equartevirus - genetics</topic><topic>Equartevirus - immunology</topic><topic>Equine arteritis virus</topic><topic>Europe</topic><topic>Genes, Viral - genetics</topic><topic>Genetic Variation - genetics</topic><topic>Genome, Viral</topic><topic>Glycoproteins - chemistry</topic><topic>Glycoproteins - genetics</topic><topic>Glycoproteins - immunology</topic><topic>Horses - virology</topic><topic>Immunodominant Epitopes - chemistry</topic><topic>Immunodominant Epitopes - genetics</topic><topic>Immunodominant Epitopes - immunology</topic><topic>Molecular Sequence Data</topic><topic>North America</topic><topic>Phylogeny</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA Replicase - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Terminology as Topic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stadejek, T</creatorcontrib><creatorcontrib>Bjorklund, H</creatorcontrib><creatorcontrib>Bascunana, CR</creatorcontrib><creatorcontrib>Ciabatti, IM</creatorcontrib><creatorcontrib>Scicluna, MT</creatorcontrib><creatorcontrib>Amaddeo, D</creatorcontrib><creatorcontrib>McCollum, WH</creatorcontrib><creatorcontrib>Autorino, GL</creatorcontrib><creatorcontrib>Timoney, PJ</creatorcontrib><creatorcontrib>Paton, DJ</creatorcontrib><creatorcontrib>Klingeborn, B</creatorcontrib><creatorcontrib>Belak, S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stadejek, T</au><au>Bjorklund, H</au><au>Bascunana, CR</au><au>Ciabatti, IM</au><au>Scicluna, MT</au><au>Amaddeo, D</au><au>McCollum, WH</au><au>Autorino, GL</au><au>Timoney, PJ</au><au>Paton, DJ</au><au>Klingeborn, B</au><au>Belak, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic diversity of equine arteritis virus</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1999-03-01</date><risdate>1999</risdate><volume>80</volume><issue>3</issue><spage>691</spage><epage>699</epage><pages>691-699</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><abstract>T Stadejek, H Bjorklund, CR Bascunana, IM Ciabatti, MT Scicluna, D Amaddeo, WH McCollum, GL Autorino, PJ Timoney, DJ Paton, B Klingeborn and S Belak
Department of Virology, The National Veterinary Institute, Biomedical Center, Uppsala, Sweden.
Equine arteritis viruses (EAV) from Europe and America were compared by
phylogenetic analysis of 43 isolates obtained over four decades. An
additional 22 virus sequences were retrieved from GenBank. Fragments of the
glycoprotein G(L) and the replicase genes were amplified by RT-PCR, prior
to sequencing and construction of phylogenetic trees. The trees revealed
many distinctive lineages, consistent with prolonged diversification within
geographically separated host populations. Two large groups and five
subgroups were distinguished. Group I consisted mainly of viruses from
North America, whilst group II consisted mainly of European isolates. In
most instances, where the geographic origin of the viruses appeared to be
at variance with the phylogenetically predicted relationships, the horses
from which the viruses were recovered had been transported between Europe
and America or vice versa. Analysis of the replicase gene revealed similar
phylogenetic relationships although not all of the groups were as clearly
defined. Virus strains CH1 (Switzerland, 1964) and S1 (Sweden, 1989)
represented separate 'outgroups' based on analysis of both genomic regions.
The results of this study confirm the value of the G(L) gene of EAV for
estimating virus genetic diversity and as a useful tool for tracing routes
by which EAV is spread. In addition, computer-assisted predictions of
antigenic sites on the G(L) protein revealed considerable variability among
the isolates, especially with respect to regions associated with
neutralization domains.</abstract><cop>England</cop><pub>Soc General Microbiol</pub><pmid>10092009</pmid><doi>10.1099/0022-1317-80-3-691</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1317 |
| ispartof | Journal of general virology, 1999-03, Vol.80 (3), p.691-699 |
| issn | 0022-1317 1465-2099 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69645823 |
| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Animals Antibodies, Viral - immunology Antigens, Viral - chemistry Antigens, Viral - genetics Antigens, Viral - immunology Arterivirus Infections - immunology Arterivirus Infections - transmission Arterivirus Infections - veterinary Arterivirus Infections - virology Cell Line Equartevirus - classification Equartevirus - genetics Equartevirus - immunology Equine arteritis virus Europe Genes, Viral - genetics Genetic Variation - genetics Genome, Viral Glycoproteins - chemistry Glycoproteins - genetics Glycoproteins - immunology Horses - virology Immunodominant Epitopes - chemistry Immunodominant Epitopes - genetics Immunodominant Epitopes - immunology Molecular Sequence Data North America Phylogeny Reverse Transcriptase Polymerase Chain Reaction RNA Replicase - genetics Sequence Analysis, DNA Terminology as Topic |
| title | Genetic diversity of equine arteritis virus |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T23%3A46%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Genetic%20diversity%20of%20equine%20arteritis%20virus&rft.jtitle=Journal%20of%20general%20virology&rft.au=Stadejek,%20T&rft.date=1999-03-01&rft.volume=80&rft.issue=3&rft.spage=691&rft.epage=699&rft.pages=691-699&rft.issn=0022-1317&rft.eissn=1465-2099&rft_id=info:doi/10.1099/0022-1317-80-3-691&rft_dat=%3Cproquest_cross%3E69645823%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17261350&rft_id=info:pmid/10092009&rfr_iscdi=true |