Phospholipases A2 in Gastric Juice of Helicobacter pylori – Positive and Negative Individuals
Gastric juice is known to have phospholipase A2 catalytic activity. Helicobacter pylori (H. pylori) has been reported to produce phospholipase A2, which is believed to hydrolyse the protective layer of gastric mucosal phospholipids and to promote mucosal damage. The current study aimed at identifyin...
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| Veröffentlicht in: | Clinical chemistry and laboratory medicine 1999-01, Vol.37 (1), p.61-64 |
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| creator | Huhtinen, Heikki T. Grönroos, Juha M. Haapamäki, Markku M. Nevalainen, Timo J. |
| description | Gastric juice is known to have phospholipase A2 catalytic activity. Helicobacter pylori (H. pylori) has been reported to produce phospholipase A2, which is believed to hydrolyse the protective layer of gastric mucosal phospholipids and to promote mucosal damage. The current study aimed at identifying secretory phospholipase A2 subtypes (pancreatic group I phospholipase A2 and synovial-type group II phospholipase A2) in gastric juice and their relation to the presence of H. pyloriin gastric mucosal biopsies in the same individuals. Gastric juice was collected from 29 individuals during gastroscopy. Biopsies were taken from the antrum and body of the stomach to determine the H. pylori status. We found catalytically active phospholipase A2 and both group I and group II phospholipases A2 in the gastric juice samples. The catalytic activity and the mass concentrations of group I and group II phospholipases A2 correlated significantly with the pH value in gastric juice. The gastric juice of H. pylori positive individuals did not contain higher amounts of phospholipases A2 than the juice of H. pylori negative individuals. Rather, the mass concentration of group II phospholipase A2 in gastric juice seemed to be somewhat lower in individuals with H. pylori infection than in uninfected individuals. The results of the current study show that both group I and group II phospholipases A2 are present in gastric juice. The main sources of phospholipases A2 in gastric juice are probably other than H. pylori. |
| doi_str_mv | 10.1515/CCLM.1999.009 |
| format | Article |
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Helicobacter pylori (H. pylori) has been reported to produce phospholipase A2, which is believed to hydrolyse the protective layer of gastric mucosal phospholipids and to promote mucosal damage. The current study aimed at identifying secretory phospholipase A2 subtypes (pancreatic group I phospholipase A2 and synovial-type group II phospholipase A2) in gastric juice and their relation to the presence of H. pyloriin gastric mucosal biopsies in the same individuals. Gastric juice was collected from 29 individuals during gastroscopy. Biopsies were taken from the antrum and body of the stomach to determine the H. pylori status. We found catalytically active phospholipase A2 and both group I and group II phospholipases A2 in the gastric juice samples. The catalytic activity and the mass concentrations of group I and group II phospholipases A2 correlated significantly with the pH value in gastric juice. The gastric juice of H. pylori positive individuals did not contain higher amounts of phospholipases A2 than the juice of H. pylori negative individuals. Rather, the mass concentration of group II phospholipase A2 in gastric juice seemed to be somewhat lower in individuals with H. pylori infection than in uninfected individuals. The results of the current study show that both group I and group II phospholipases A2 are present in gastric juice. The main sources of phospholipases A2 in gastric juice are probably other than H. pylori.</description><identifier>ISSN: 1434-6621</identifier><identifier>EISSN: 1437-4331</identifier><identifier>DOI: 10.1515/CCLM.1999.009</identifier><identifier>PMID: 10094380</identifier><language>eng</language><publisher>Berlin: Walter de Gruyter</publisher><subject>Adult ; Aged ; Bacterial diseases ; Bacterial diseases of the digestive system and abdomen ; Biological and medical sciences ; Catalysis ; Female ; Gastric Juice - enzymology ; Helicobacter Infections - enzymology ; Helicobacter Infections - microbiology ; Helicobacter pylori ; Helicobacter pylori - isolation & purification ; Human bacterial diseases ; Humans ; Hydrogen-Ion Concentration ; Infectious diseases ; Male ; Medical sciences ; Middle Aged ; Phospholipases A - metabolism ; Phospholipases A2</subject><ispartof>Clinical chemistry and laboratory medicine, 1999-01, Vol.37 (1), p.61-64</ispartof><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4022,27922,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1816998$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10094380$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huhtinen, Heikki T.</creatorcontrib><creatorcontrib>Grönroos, Juha M.</creatorcontrib><creatorcontrib>Haapamäki, Markku M.</creatorcontrib><creatorcontrib>Nevalainen, Timo J.</creatorcontrib><title>Phospholipases A2 in Gastric Juice of Helicobacter pylori – Positive and Negative Individuals</title><title>Clinical chemistry and laboratory medicine</title><addtitle>Clinical Chemistry and Laboratory Medicine</addtitle><description>Gastric juice is known to have phospholipase A2 catalytic activity. Helicobacter pylori (H. pylori) has been reported to produce phospholipase A2, which is believed to hydrolyse the protective layer of gastric mucosal phospholipids and to promote mucosal damage. The current study aimed at identifying secretory phospholipase A2 subtypes (pancreatic group I phospholipase A2 and synovial-type group II phospholipase A2) in gastric juice and their relation to the presence of H. pyloriin gastric mucosal biopsies in the same individuals. Gastric juice was collected from 29 individuals during gastroscopy. Biopsies were taken from the antrum and body of the stomach to determine the H. pylori status. We found catalytically active phospholipase A2 and both group I and group II phospholipases A2 in the gastric juice samples. The catalytic activity and the mass concentrations of group I and group II phospholipases A2 correlated significantly with the pH value in gastric juice. The gastric juice of H. pylori positive individuals did not contain higher amounts of phospholipases A2 than the juice of H. pylori negative individuals. Rather, the mass concentration of group II phospholipase A2 in gastric juice seemed to be somewhat lower in individuals with H. pylori infection than in uninfected individuals. The results of the current study show that both group I and group II phospholipases A2 are present in gastric juice. The main sources of phospholipases A2 in gastric juice are probably other than H. pylori.</description><subject>Adult</subject><subject>Aged</subject><subject>Bacterial diseases</subject><subject>Bacterial diseases of the digestive system and abdomen</subject><subject>Biological and medical sciences</subject><subject>Catalysis</subject><subject>Female</subject><subject>Gastric Juice - enzymology</subject><subject>Helicobacter Infections - enzymology</subject><subject>Helicobacter Infections - microbiology</subject><subject>Helicobacter pylori</subject><subject>Helicobacter pylori - isolation & purification</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Phospholipases A - metabolism</subject><subject>Phospholipases A2</subject><issn>1434-6621</issn><issn>1437-4331</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0DtPwzAQB3ALgWgpjKzIA2JL8SsPj6WiDyhQpCKxWY7tUEOahDip6MZ34BvySQhtgZHp7q_76aQ7AI4x6mIf--f9_uSmiznnXYT4DmhjRkOPUYp31z3zgoDgFjhw7hkh7Pss3Act3FhGI9QGYjrPXTHPU1tIZxzsEWgzOJSuKq2CV7VVBuYJHJnUqjyWqjIlLFZpXlr4-f4Bp7mzlV0aKDMNb82TXIdxpu3S6lqm7hDsJU0xR9vaAQ-Dy1l_5E3uhuN-b-JZQlDlKWI4ljQ2jKnYKEIlSbTBLJK0yQmLNFEJ5TgmcUgQC0OiEUm4zxgisfYV7YCzzd6izF9r4yqxsE6ZNJWZyWsnAh40BwfkX0hQhBiPogaebGEdL4wWRWkXslyJn9814HQLpFMyTUqZKev-XIQDzr_3eBtmXWXefseyfBFBSENf3M-YuGajwWNwMRCYfgFc7Y0O</recordid><startdate>19990101</startdate><enddate>19990101</enddate><creator>Huhtinen, Heikki T.</creator><creator>Grönroos, Juha M.</creator><creator>Haapamäki, Markku M.</creator><creator>Nevalainen, Timo J.</creator><general>Walter de Gruyter</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19990101</creationdate><title>Phospholipases A2 in Gastric Juice of Helicobacter pylori – Positive and Negative Individuals</title><author>Huhtinen, Heikki T. ; Grönroos, Juha M. ; Haapamäki, Markku M. ; Nevalainen, Timo J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i220t-c2e91a3be44cbec23a2fde148a3cbef48d2cf391b2b7204772d02f954402bd5c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Bacterial diseases</topic><topic>Bacterial diseases of the digestive system and abdomen</topic><topic>Biological and medical sciences</topic><topic>Catalysis</topic><topic>Female</topic><topic>Gastric Juice - enzymology</topic><topic>Helicobacter Infections - enzymology</topic><topic>Helicobacter Infections - microbiology</topic><topic>Helicobacter pylori</topic><topic>Helicobacter pylori - isolation & purification</topic><topic>Human bacterial diseases</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Infectious diseases</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Phospholipases A - metabolism</topic><topic>Phospholipases A2</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huhtinen, Heikki T.</creatorcontrib><creatorcontrib>Grönroos, Juha M.</creatorcontrib><creatorcontrib>Haapamäki, Markku M.</creatorcontrib><creatorcontrib>Nevalainen, Timo J.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical chemistry and laboratory medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huhtinen, Heikki T.</au><au>Grönroos, Juha M.</au><au>Haapamäki, Markku M.</au><au>Nevalainen, Timo J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phospholipases A2 in Gastric Juice of Helicobacter pylori – Positive and Negative Individuals</atitle><jtitle>Clinical chemistry and laboratory medicine</jtitle><addtitle>Clinical Chemistry and Laboratory Medicine</addtitle><date>1999-01-01</date><risdate>1999</risdate><volume>37</volume><issue>1</issue><spage>61</spage><epage>64</epage><pages>61-64</pages><issn>1434-6621</issn><eissn>1437-4331</eissn><abstract>Gastric juice is known to have phospholipase A2 catalytic activity. Helicobacter pylori (H. pylori) has been reported to produce phospholipase A2, which is believed to hydrolyse the protective layer of gastric mucosal phospholipids and to promote mucosal damage. The current study aimed at identifying secretory phospholipase A2 subtypes (pancreatic group I phospholipase A2 and synovial-type group II phospholipase A2) in gastric juice and their relation to the presence of H. pyloriin gastric mucosal biopsies in the same individuals. Gastric juice was collected from 29 individuals during gastroscopy. Biopsies were taken from the antrum and body of the stomach to determine the H. pylori status. We found catalytically active phospholipase A2 and both group I and group II phospholipases A2 in the gastric juice samples. The catalytic activity and the mass concentrations of group I and group II phospholipases A2 correlated significantly with the pH value in gastric juice. The gastric juice of H. pylori positive individuals did not contain higher amounts of phospholipases A2 than the juice of H. pylori negative individuals. Rather, the mass concentration of group II phospholipase A2 in gastric juice seemed to be somewhat lower in individuals with H. pylori infection than in uninfected individuals. The results of the current study show that both group I and group II phospholipases A2 are present in gastric juice. The main sources of phospholipases A2 in gastric juice are probably other than H. pylori.</abstract><cop>Berlin</cop><cop>New York, NY</cop><pub>Walter de Gruyter</pub><pmid>10094380</pmid><doi>10.1515/CCLM.1999.009</doi><tpages>4</tpages></addata></record> |
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| source | MEDLINE; De Gruyter journals |
| subjects | Adult Aged Bacterial diseases Bacterial diseases of the digestive system and abdomen Biological and medical sciences Catalysis Female Gastric Juice - enzymology Helicobacter Infections - enzymology Helicobacter Infections - microbiology Helicobacter pylori Helicobacter pylori - isolation & purification Human bacterial diseases Humans Hydrogen-Ion Concentration Infectious diseases Male Medical sciences Middle Aged Phospholipases A - metabolism Phospholipases A2 |
| title | Phospholipases A2 in Gastric Juice of Helicobacter pylori – Positive and Negative Individuals |
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