Preliminary X-ray crystallographic analysis of the complex between the DNAase domain of colicin E9 and its cognate immunity protein

We have crystallized and performed preliminary X‐ray characterization of the complex between the DNAase domain of the E9 colicin and its cognate immunity protein Im9. The dissociation constant for this complex, Kd = 1 × 10−16 M, reveals it to be one of the highest affinity protein–protein interactio...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 1999-01, Vol.55 (1), p.256-259
Hauptverfasser:	Kühlmann, Ulrike C., Kleanthous, Colin, James, Richard, Moore, Geoffrey R., Hemmings, Andrew M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - chemistry Bacterial Proteins - genetics Bacterial Proteins - isolation & purification Binding Sites colicin E9-cognate immunity protein Im9 Colicins - chemistry Colicins - genetics Colicins - isolation & purification Crystallization Crystallography, X-Ray Deoxyribonucleases Escherichia coli - genetics Escherichia coli Proteins Macromolecular Substances
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!