Circulating DNA in the Blood of Gastric Cancer Patients
The concentration of cell‐free DNA and promoter methylation status of the MGMT, p15, and hMLH1 genes were analyzed by a fluorescence‐based assay and methylation‐specific PCR (MSP) in the blood of gastric cancer patients (n= 20) and healthy subjects (n= 22). Gastric cancer patients were characterized...
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| Veröffentlicht in: | Annals of the New York Academy of Sciences 2008-08, Vol.1137 (1), p.226-231 |
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| container_title | Annals of the New York Academy of Sciences |
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| creator | Kolesnikova, Elena V. Tamkovich, Svetlana N. Bryzgunova, Olga E. Shelestyuk, Petr I. Permyakova, Valentina I. Vlassov, Valentin V. Tuzikov, Aleksandr S. Laktionov, Pavel P. Rykova, Elena Y. |
| description | The concentration of cell‐free DNA and promoter methylation status of the MGMT, p15, and hMLH1 genes were analyzed by a fluorescence‐based assay and methylation‐specific PCR (MSP) in the blood of gastric cancer patients (n= 20) and healthy subjects (n= 22). Gastric cancer patients were characterized by an increased concentration of circulating DNA in the plasma; the amount of cell‐surface‐bound DNA was not decreased compared with controls and amounted to 80 ± 15% of the total circulating DNA. MSP analysis of three genes in the cell‐surface‐bound DNA permits the detection of gastric cancer patients with a sensitivity of 75% and a specificity of 54%. Thus, the cell‐surface‐bound DNA is a convenient source of DNA for MSP analysis of cancer‐specific markers. The data on the presence of methylated DNA in plasma combined with the analysis of other cancer‐related changes in DNA can significantly contribute to cancer diagnostics. |
| doi_str_mv | 10.1196/annals.1448.009 |
| format | Article |
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Gastric cancer patients were characterized by an increased concentration of circulating DNA in the plasma; the amount of cell‐surface‐bound DNA was not decreased compared with controls and amounted to 80 ± 15% of the total circulating DNA. MSP analysis of three genes in the cell‐surface‐bound DNA permits the detection of gastric cancer patients with a sensitivity of 75% and a specificity of 54%. Thus, the cell‐surface‐bound DNA is a convenient source of DNA for MSP analysis of cancer‐specific markers. The data on the presence of methylated DNA in plasma combined with the analysis of other cancer‐related changes in DNA can significantly contribute to cancer diagnostics.</description><identifier>ISSN: 0077-8923</identifier><identifier>EISSN: 1749-6632</identifier><identifier>EISSN: 1930-6547</identifier><identifier>DOI: 10.1196/annals.1448.009</identifier><identifier>PMID: 18837952</identifier><language>eng</language><publisher>Malden, USA: Blackwell Publishing Inc</publisher><subject>Adaptor Proteins, Signal Transducing - genetics ; Carrier Proteins - genetics ; cell-surface-bound DNA ; circulating DNA ; DNA - blood ; DNA - genetics ; DNA Methylation ; DNA Modification Methylases - genetics ; DNA Repair Enzymes - genetics ; Female ; gastric cancer ; Humans ; hypermethylation ; Male ; Middle Aged ; MutL Protein Homolog 1 ; Nuclear Proteins - genetics ; Promoter Regions, Genetic ; Stomach Neoplasms - blood ; Stomach Neoplasms - diagnosis ; Stomach Neoplasms - genetics ; Tumor Suppressor Proteins - genetics</subject><ispartof>Annals of the New York Academy of Sciences, 2008-08, Vol.1137 (1), p.226-231</ispartof><rights>2008 New York Academy of Sciences</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4799-eae1545fa27337d810e9aa0a155ddbe71ef840116a82d7824e5bf94fa946c46e3</citedby><cites>FETCH-LOGICAL-c4799-eae1545fa27337d810e9aa0a155ddbe71ef840116a82d7824e5bf94fa946c46e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1196%2Fannals.1448.009$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1196%2Fannals.1448.009$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18837952$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kolesnikova, Elena V.</creatorcontrib><creatorcontrib>Tamkovich, Svetlana N.</creatorcontrib><creatorcontrib>Bryzgunova, Olga E.</creatorcontrib><creatorcontrib>Shelestyuk, Petr I.</creatorcontrib><creatorcontrib>Permyakova, Valentina I.</creatorcontrib><creatorcontrib>Vlassov, Valentin V.</creatorcontrib><creatorcontrib>Tuzikov, Aleksandr S.</creatorcontrib><creatorcontrib>Laktionov, Pavel P.</creatorcontrib><creatorcontrib>Rykova, Elena Y.</creatorcontrib><title>Circulating DNA in the Blood of Gastric Cancer Patients</title><title>Annals of the New York Academy of Sciences</title><addtitle>Ann N Y Acad Sci</addtitle><description>The concentration of cell‐free DNA and promoter methylation status of the MGMT, p15, and hMLH1 genes were analyzed by a fluorescence‐based assay and methylation‐specific PCR (MSP) in the blood of gastric cancer patients (n= 20) and healthy subjects (n= 22). Gastric cancer patients were characterized by an increased concentration of circulating DNA in the plasma; the amount of cell‐surface‐bound DNA was not decreased compared with controls and amounted to 80 ± 15% of the total circulating DNA. MSP analysis of three genes in the cell‐surface‐bound DNA permits the detection of gastric cancer patients with a sensitivity of 75% and a specificity of 54%. Thus, the cell‐surface‐bound DNA is a convenient source of DNA for MSP analysis of cancer‐specific markers. The data on the presence of methylated DNA in plasma combined with the analysis of other cancer‐related changes in DNA can significantly contribute to cancer diagnostics.</description><subject>Adaptor Proteins, Signal Transducing - genetics</subject><subject>Carrier Proteins - genetics</subject><subject>cell-surface-bound DNA</subject><subject>circulating DNA</subject><subject>DNA - blood</subject><subject>DNA - genetics</subject><subject>DNA Methylation</subject><subject>DNA Modification Methylases - genetics</subject><subject>DNA Repair Enzymes - genetics</subject><subject>Female</subject><subject>gastric cancer</subject><subject>Humans</subject><subject>hypermethylation</subject><subject>Male</subject><subject>Middle Aged</subject><subject>MutL Protein Homolog 1</subject><subject>Nuclear Proteins - genetics</subject><subject>Promoter Regions, Genetic</subject><subject>Stomach Neoplasms - blood</subject><subject>Stomach Neoplasms - diagnosis</subject><subject>Stomach Neoplasms - genetics</subject><subject>Tumor Suppressor Proteins - genetics</subject><issn>0077-8923</issn><issn>1749-6632</issn><issn>1930-6547</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFPwjAUhxujEUTP3sxO3gbt2vW1R0RFDaIJGuOpKdubTseG7Yjy3zsyokdOvXy_76UfIaeM9hnTcmDL0ha-z4RQfUr1HukyEDqUkkf7pEspQKh0xDvkyPsPSlmkBBySDlOKg46jLoFR7pJVYeu8fAsup8MgL4P6HYOLoqrSoMqCsfW1y5NgZMsEXfDYkFjW_pgcZM1lPNm-PfJ8ffU0ugknD-Pb0XASJgK0DtEii0Wc2Qg4h1Qxitpaalkcp-kcgWGmBGVMWhWloCKB8TzTIrNayERI5D1y3nqXrvpaoa_NIvcJFoUtsVp5I7UUFDTfCUZUyHjz7x4ZtGDiKu8dZmbp8oV1a8Oo2UQ1bVSziWqaqM3ibKtezReY_vPbig2gWuA7L3C9y2emr8MZYxxad9hOc1_jz9_Uuk8jgUNsXqZjM5ndz-AOJobzX0hsko4</recordid><startdate>200808</startdate><enddate>200808</enddate><creator>Kolesnikova, Elena V.</creator><creator>Tamkovich, Svetlana N.</creator><creator>Bryzgunova, Olga E.</creator><creator>Shelestyuk, Petr I.</creator><creator>Permyakova, Valentina I.</creator><creator>Vlassov, Valentin V.</creator><creator>Tuzikov, Aleksandr S.</creator><creator>Laktionov, Pavel P.</creator><creator>Rykova, Elena Y.</creator><general>Blackwell Publishing Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>200808</creationdate><title>Circulating DNA in the Blood of Gastric Cancer Patients</title><author>Kolesnikova, Elena V. ; Tamkovich, Svetlana N. ; Bryzgunova, Olga E. ; Shelestyuk, Petr I. ; Permyakova, Valentina I. ; Vlassov, Valentin V. ; Tuzikov, Aleksandr S. ; Laktionov, Pavel P. ; Rykova, Elena Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4799-eae1545fa27337d810e9aa0a155ddbe71ef840116a82d7824e5bf94fa946c46e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adaptor Proteins, Signal Transducing - genetics</topic><topic>Carrier Proteins - genetics</topic><topic>cell-surface-bound DNA</topic><topic>circulating DNA</topic><topic>DNA - blood</topic><topic>DNA - genetics</topic><topic>DNA Methylation</topic><topic>DNA Modification Methylases - genetics</topic><topic>DNA Repair Enzymes - genetics</topic><topic>Female</topic><topic>gastric cancer</topic><topic>Humans</topic><topic>hypermethylation</topic><topic>Male</topic><topic>Middle Aged</topic><topic>MutL Protein Homolog 1</topic><topic>Nuclear Proteins - genetics</topic><topic>Promoter Regions, Genetic</topic><topic>Stomach Neoplasms - blood</topic><topic>Stomach Neoplasms - diagnosis</topic><topic>Stomach Neoplasms - genetics</topic><topic>Tumor Suppressor Proteins - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kolesnikova, Elena V.</creatorcontrib><creatorcontrib>Tamkovich, Svetlana N.</creatorcontrib><creatorcontrib>Bryzgunova, Olga E.</creatorcontrib><creatorcontrib>Shelestyuk, Petr I.</creatorcontrib><creatorcontrib>Permyakova, Valentina I.</creatorcontrib><creatorcontrib>Vlassov, Valentin V.</creatorcontrib><creatorcontrib>Tuzikov, Aleksandr S.</creatorcontrib><creatorcontrib>Laktionov, Pavel P.</creatorcontrib><creatorcontrib>Rykova, Elena Y.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of the New York Academy of Sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kolesnikova, Elena V.</au><au>Tamkovich, Svetlana N.</au><au>Bryzgunova, Olga E.</au><au>Shelestyuk, Petr I.</au><au>Permyakova, Valentina I.</au><au>Vlassov, Valentin V.</au><au>Tuzikov, Aleksandr S.</au><au>Laktionov, Pavel P.</au><au>Rykova, Elena Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Circulating DNA in the Blood of Gastric Cancer Patients</atitle><jtitle>Annals of the New York Academy of Sciences</jtitle><addtitle>Ann N Y Acad Sci</addtitle><date>2008-08</date><risdate>2008</risdate><volume>1137</volume><issue>1</issue><spage>226</spage><epage>231</epage><pages>226-231</pages><issn>0077-8923</issn><eissn>1749-6632</eissn><eissn>1930-6547</eissn><abstract>The concentration of cell‐free DNA and promoter methylation status of the MGMT, p15, and hMLH1 genes were analyzed by a fluorescence‐based assay and methylation‐specific PCR (MSP) in the blood of gastric cancer patients (n= 20) and healthy subjects (n= 22). Gastric cancer patients were characterized by an increased concentration of circulating DNA in the plasma; the amount of cell‐surface‐bound DNA was not decreased compared with controls and amounted to 80 ± 15% of the total circulating DNA. MSP analysis of three genes in the cell‐surface‐bound DNA permits the detection of gastric cancer patients with a sensitivity of 75% and a specificity of 54%. Thus, the cell‐surface‐bound DNA is a convenient source of DNA for MSP analysis of cancer‐specific markers. The data on the presence of methylated DNA in plasma combined with the analysis of other cancer‐related changes in DNA can significantly contribute to cancer diagnostics.</abstract><cop>Malden, USA</cop><pub>Blackwell Publishing Inc</pub><pmid>18837952</pmid><doi>10.1196/annals.1448.009</doi><tpages>6</tpages></addata></record> |
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| subjects | Adaptor Proteins, Signal Transducing - genetics Carrier Proteins - genetics cell-surface-bound DNA circulating DNA DNA - blood DNA - genetics DNA Methylation DNA Modification Methylases - genetics DNA Repair Enzymes - genetics Female gastric cancer Humans hypermethylation Male Middle Aged MutL Protein Homolog 1 Nuclear Proteins - genetics Promoter Regions, Genetic Stomach Neoplasms - blood Stomach Neoplasms - diagnosis Stomach Neoplasms - genetics Tumor Suppressor Proteins - genetics |
| title | Circulating DNA in the Blood of Gastric Cancer Patients |
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