Influence of surgical trauma on the mRNA expression of sex hormone receptors in PBMCs in male and female patients
Introduction Gender-specific immune responses have been found after trauma-hemorrhage. Male and female sex hormones seem to be responsible for this gender dimorphism. Alterations in sex hormone receptor expression in mice appear to contribute to the immunomodulatory effect of sex hormones after bloo...
Gespeichert in:
| Veröffentlicht in: | Langenbeck's archives of surgery 2008-11, Vol.393 (6), p.871-876 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 876 |
|---|---|
| container_issue | 6 |
| container_start_page | 871 |
| container_title | Langenbeck's archives of surgery |
| container_volume | 393 |
| creator | Brechenmacher, S. A. Bruns, C. J. Van den Engel, N. K. Angele, P. Loehe, F. Jauch, K.-W. Angele, M. K. |
| description | Introduction
Gender-specific immune responses have been found after trauma-hemorrhage. Male and female sex hormones seem to be responsible for this gender dimorphism. Alterations in sex hormone receptor expression in mice appear to contribute to the immunomodulatory effect of sex hormones after blood loss. The effect of surgical trauma on the expression of sex hormone receptors in peripheral blood mononuclear cells (PBMCs) from patients, however, remains unknown.
Materials and methods
PBMCs were obtained from 14 patients (7 men and 7 women) undergoing major abdominal surgery preoperatively and 2 h postoperatively. The expression of the androgen and the estrogen α- and β- receptors were determined by reverse transcriptase polymerase chain reaction (RT-PCR). β-Actin was used as housekeeping gene.
Results
The results indicate that surgical trauma has no influence on the expression of the androgen receptor and the estrogen receptors α and β in male and female patients.
Discussion
The data demonstrate that, in contrast to mice, no alterations in the expression of androgen and estrogen hormone receptors were evident after surgery in patients. Thus, differences in the expression of sex hormone receptors do not appear to be responsible for the gender-specific immune response after surgery. |
| doi_str_mv | 10.1007/s00423-008-0304-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69640333</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69640333</sourcerecordid><originalsourceid>FETCH-LOGICAL-c342t-c225ad2280b3ff2b235bce4378355a2f39e6f445266c4468ef0c4c2782e8941e3</originalsourceid><addsrcrecordid>eNp9kEtPwzAQhC0EolD4AVyQT9wCju0kzrFUPCqVhxCcLcddt6kSO7UTqfx70qaCG3vZkT0z0n4IXcXkNiYkuwuEcMoiQkREGOGROEJnMWdJRHkSH_9qzkboPIQ1ISTNcn6KRrGgedYnztBmZk3VgdWAncGh88tSqwq3XnW1ws7idgW4_nidYNg2HkIo-7edE7Z45XztLGAPGprW-YBLi9_vX6Z7UasKsLILbGAvG9WWYNtwgU6MqgJcHvYYfT0-fE6fo_nb02w6mUeacdpGmtJELSgVpGDG0IKypNDAWSZYkihqWA6p4Tyhaao5TwUYormmmaAgch4DG6ObobfxbtNBaGVdBg1VpSy4Lsg0Tzlh_YxRPBi1dyF4MLLxZa38t4yJ3HGWA2fZc5Y7zlL0metDeVfUsPhLHMD2BjoYQv9ll-Dl2nXe9gf_0_oDFXGIEg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69640333</pqid></control><display><type>article</type><title>Influence of surgical trauma on the mRNA expression of sex hormone receptors in PBMCs in male and female patients</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Brechenmacher, S. A. ; Bruns, C. J. ; Van den Engel, N. K. ; Angele, P. ; Loehe, F. ; Jauch, K.-W. ; Angele, M. K.</creator><creatorcontrib>Brechenmacher, S. A. ; Bruns, C. J. ; Van den Engel, N. K. ; Angele, P. ; Loehe, F. ; Jauch, K.-W. ; Angele, M. K.</creatorcontrib><description>Introduction
Gender-specific immune responses have been found after trauma-hemorrhage. Male and female sex hormones seem to be responsible for this gender dimorphism. Alterations in sex hormone receptor expression in mice appear to contribute to the immunomodulatory effect of sex hormones after blood loss. The effect of surgical trauma on the expression of sex hormone receptors in peripheral blood mononuclear cells (PBMCs) from patients, however, remains unknown.
Materials and methods
PBMCs were obtained from 14 patients (7 men and 7 women) undergoing major abdominal surgery preoperatively and 2 h postoperatively. The expression of the androgen and the estrogen α- and β- receptors were determined by reverse transcriptase polymerase chain reaction (RT-PCR). β-Actin was used as housekeeping gene.
Results
The results indicate that surgical trauma has no influence on the expression of the androgen receptor and the estrogen receptors α and β in male and female patients.
Discussion
The data demonstrate that, in contrast to mice, no alterations in the expression of androgen and estrogen hormone receptors were evident after surgery in patients. Thus, differences in the expression of sex hormone receptors do not appear to be responsible for the gender-specific immune response after surgery.</description><identifier>ISSN: 1435-2443</identifier><identifier>EISSN: 1435-2451</identifier><identifier>DOI: 10.1007/s00423-008-0304-8</identifier><identifier>PMID: 18297304</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Abdominal Surgery ; Adult ; Aged ; Blood Loss, Surgical - physiopathology ; Cardiac Surgery ; Colorectal Neoplasms - surgery ; Estrogen Receptor alpha - genetics ; Estrogen Receptor beta - genetics ; Female ; General Surgery ; Humans ; Liver Neoplasms - secondary ; Liver Neoplasms - surgery ; Male ; Medicine ; Medicine & Public Health ; Middle Aged ; Monocytes - metabolism ; Original Article ; Pancreatic Neoplasms - surgery ; Receptors, Androgen - genetics ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; Shock, Hemorrhagic - genetics ; Thoracic Surgery ; Traumatic Surgery ; Vascular Surgery</subject><ispartof>Langenbeck's archives of surgery, 2008-11, Vol.393 (6), p.871-876</ispartof><rights>Springer-Verlag 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c342t-c225ad2280b3ff2b235bce4378355a2f39e6f445266c4468ef0c4c2782e8941e3</citedby><cites>FETCH-LOGICAL-c342t-c225ad2280b3ff2b235bce4378355a2f39e6f445266c4468ef0c4c2782e8941e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00423-008-0304-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00423-008-0304-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18297304$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brechenmacher, S. A.</creatorcontrib><creatorcontrib>Bruns, C. J.</creatorcontrib><creatorcontrib>Van den Engel, N. K.</creatorcontrib><creatorcontrib>Angele, P.</creatorcontrib><creatorcontrib>Loehe, F.</creatorcontrib><creatorcontrib>Jauch, K.-W.</creatorcontrib><creatorcontrib>Angele, M. K.</creatorcontrib><title>Influence of surgical trauma on the mRNA expression of sex hormone receptors in PBMCs in male and female patients</title><title>Langenbeck's archives of surgery</title><addtitle>Langenbecks Arch Surg</addtitle><addtitle>Langenbecks Arch Surg</addtitle><description>Introduction
Gender-specific immune responses have been found after trauma-hemorrhage. Male and female sex hormones seem to be responsible for this gender dimorphism. Alterations in sex hormone receptor expression in mice appear to contribute to the immunomodulatory effect of sex hormones after blood loss. The effect of surgical trauma on the expression of sex hormone receptors in peripheral blood mononuclear cells (PBMCs) from patients, however, remains unknown.
Materials and methods
PBMCs were obtained from 14 patients (7 men and 7 women) undergoing major abdominal surgery preoperatively and 2 h postoperatively. The expression of the androgen and the estrogen α- and β- receptors were determined by reverse transcriptase polymerase chain reaction (RT-PCR). β-Actin was used as housekeeping gene.
Results
The results indicate that surgical trauma has no influence on the expression of the androgen receptor and the estrogen receptors α and β in male and female patients.
Discussion
The data demonstrate that, in contrast to mice, no alterations in the expression of androgen and estrogen hormone receptors were evident after surgery in patients. Thus, differences in the expression of sex hormone receptors do not appear to be responsible for the gender-specific immune response after surgery.</description><subject>Abdominal Surgery</subject><subject>Adult</subject><subject>Aged</subject><subject>Blood Loss, Surgical - physiopathology</subject><subject>Cardiac Surgery</subject><subject>Colorectal Neoplasms - surgery</subject><subject>Estrogen Receptor alpha - genetics</subject><subject>Estrogen Receptor beta - genetics</subject><subject>Female</subject><subject>General Surgery</subject><subject>Humans</subject><subject>Liver Neoplasms - secondary</subject><subject>Liver Neoplasms - surgery</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Middle Aged</subject><subject>Monocytes - metabolism</subject><subject>Original Article</subject><subject>Pancreatic Neoplasms - surgery</subject><subject>Receptors, Androgen - genetics</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - genetics</subject><subject>Shock, Hemorrhagic - genetics</subject><subject>Thoracic Surgery</subject><subject>Traumatic Surgery</subject><subject>Vascular Surgery</subject><issn>1435-2443</issn><issn>1435-2451</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtPwzAQhC0EolD4AVyQT9wCju0kzrFUPCqVhxCcLcddt6kSO7UTqfx70qaCG3vZkT0z0n4IXcXkNiYkuwuEcMoiQkREGOGROEJnMWdJRHkSH_9qzkboPIQ1ISTNcn6KRrGgedYnztBmZk3VgdWAncGh88tSqwq3XnW1ws7idgW4_nidYNg2HkIo-7edE7Z45XztLGAPGprW-YBLi9_vX6Z7UasKsLILbGAvG9WWYNtwgU6MqgJcHvYYfT0-fE6fo_nb02w6mUeacdpGmtJELSgVpGDG0IKypNDAWSZYkihqWA6p4Tyhaao5TwUYormmmaAgch4DG6ObobfxbtNBaGVdBg1VpSy4Lsg0Tzlh_YxRPBi1dyF4MLLxZa38t4yJ3HGWA2fZc5Y7zlL0metDeVfUsPhLHMD2BjoYQv9ll-Dl2nXe9gf_0_oDFXGIEg</recordid><startdate>20081101</startdate><enddate>20081101</enddate><creator>Brechenmacher, S. A.</creator><creator>Bruns, C. J.</creator><creator>Van den Engel, N. K.</creator><creator>Angele, P.</creator><creator>Loehe, F.</creator><creator>Jauch, K.-W.</creator><creator>Angele, M. K.</creator><general>Springer-Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20081101</creationdate><title>Influence of surgical trauma on the mRNA expression of sex hormone receptors in PBMCs in male and female patients</title><author>Brechenmacher, S. A. ; Bruns, C. J. ; Van den Engel, N. K. ; Angele, P. ; Loehe, F. ; Jauch, K.-W. ; Angele, M. K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c342t-c225ad2280b3ff2b235bce4378355a2f39e6f445266c4468ef0c4c2782e8941e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Abdominal Surgery</topic><topic>Adult</topic><topic>Aged</topic><topic>Blood Loss, Surgical - physiopathology</topic><topic>Cardiac Surgery</topic><topic>Colorectal Neoplasms - surgery</topic><topic>Estrogen Receptor alpha - genetics</topic><topic>Estrogen Receptor beta - genetics</topic><topic>Female</topic><topic>General Surgery</topic><topic>Humans</topic><topic>Liver Neoplasms - secondary</topic><topic>Liver Neoplasms - surgery</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Middle Aged</topic><topic>Monocytes - metabolism</topic><topic>Original Article</topic><topic>Pancreatic Neoplasms - surgery</topic><topic>Receptors, Androgen - genetics</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - genetics</topic><topic>Shock, Hemorrhagic - genetics</topic><topic>Thoracic Surgery</topic><topic>Traumatic Surgery</topic><topic>Vascular Surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brechenmacher, S. A.</creatorcontrib><creatorcontrib>Bruns, C. J.</creatorcontrib><creatorcontrib>Van den Engel, N. K.</creatorcontrib><creatorcontrib>Angele, P.</creatorcontrib><creatorcontrib>Loehe, F.</creatorcontrib><creatorcontrib>Jauch, K.-W.</creatorcontrib><creatorcontrib>Angele, M. K.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Langenbeck's archives of surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brechenmacher, S. A.</au><au>Bruns, C. J.</au><au>Van den Engel, N. K.</au><au>Angele, P.</au><au>Loehe, F.</au><au>Jauch, K.-W.</au><au>Angele, M. K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of surgical trauma on the mRNA expression of sex hormone receptors in PBMCs in male and female patients</atitle><jtitle>Langenbeck's archives of surgery</jtitle><stitle>Langenbecks Arch Surg</stitle><addtitle>Langenbecks Arch Surg</addtitle><date>2008-11-01</date><risdate>2008</risdate><volume>393</volume><issue>6</issue><spage>871</spage><epage>876</epage><pages>871-876</pages><issn>1435-2443</issn><eissn>1435-2451</eissn><abstract>Introduction
Gender-specific immune responses have been found after trauma-hemorrhage. Male and female sex hormones seem to be responsible for this gender dimorphism. Alterations in sex hormone receptor expression in mice appear to contribute to the immunomodulatory effect of sex hormones after blood loss. The effect of surgical trauma on the expression of sex hormone receptors in peripheral blood mononuclear cells (PBMCs) from patients, however, remains unknown.
Materials and methods
PBMCs were obtained from 14 patients (7 men and 7 women) undergoing major abdominal surgery preoperatively and 2 h postoperatively. The expression of the androgen and the estrogen α- and β- receptors were determined by reverse transcriptase polymerase chain reaction (RT-PCR). β-Actin was used as housekeeping gene.
Results
The results indicate that surgical trauma has no influence on the expression of the androgen receptor and the estrogen receptors α and β in male and female patients.
Discussion
The data demonstrate that, in contrast to mice, no alterations in the expression of androgen and estrogen hormone receptors were evident after surgery in patients. Thus, differences in the expression of sex hormone receptors do not appear to be responsible for the gender-specific immune response after surgery.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>18297304</pmid><doi>10.1007/s00423-008-0304-8</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1435-2443 |
| ispartof | Langenbeck's archives of surgery, 2008-11, Vol.393 (6), p.871-876 |
| issn | 1435-2443 1435-2451 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69640333 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Abdominal Surgery Adult Aged Blood Loss, Surgical - physiopathology Cardiac Surgery Colorectal Neoplasms - surgery Estrogen Receptor alpha - genetics Estrogen Receptor beta - genetics Female General Surgery Humans Liver Neoplasms - secondary Liver Neoplasms - surgery Male Medicine Medicine & Public Health Middle Aged Monocytes - metabolism Original Article Pancreatic Neoplasms - surgery Receptors, Androgen - genetics Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics Shock, Hemorrhagic - genetics Thoracic Surgery Traumatic Surgery Vascular Surgery |
| title | Influence of surgical trauma on the mRNA expression of sex hormone receptors in PBMCs in male and female patients |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T15%3A32%3A22IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Influence%20of%20surgical%20trauma%20on%20the%20mRNA%20expression%20of%20sex%20hormone%20receptors%20in%20PBMCs%20in%20male%20and%20female%20patients&rft.jtitle=Langenbeck's%20archives%20of%20surgery&rft.au=Brechenmacher,%20S.%20A.&rft.date=2008-11-01&rft.volume=393&rft.issue=6&rft.spage=871&rft.epage=876&rft.pages=871-876&rft.issn=1435-2443&rft.eissn=1435-2451&rft_id=info:doi/10.1007/s00423-008-0304-8&rft_dat=%3Cproquest_cross%3E69640333%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=69640333&rft_id=info:pmid/18297304&rfr_iscdi=true |