Localization of Carbonic Anhydrase in Living Osteoclasts with Bodipy 558/568-modified Acetazolamide, a Thiadiazole Carbonic Anhydrase Inhibitor

We describe the synthesis of Bodipy 558/568-modified acetazolamide, a fluorescent inhibitor of carbonic anhydrase and its use to localize the enzyme in living cells. The modified acetazolamide, with its specific sulfonamide group intact, labeled cells at concentrations as low as 10−9 M, with a minim...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	The journal of histochemistry and cytochemistry 1999-04, Vol.47 (4), p.545-550
Hauptverfasser:	Brubaker, Kristen D, Mao, Fei, Gay, Carol V
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetazolamide - metabolism Animals Binding, Competitive Boron Compounds - metabolism Carbonic Anhydrase Inhibitors - metabolism Carbonic Anhydrase Inhibitors - pharmacology Carbonic Anhydrases - metabolism Chickens Dose-Response Relationship, Drug Fluorescent Dyes - metabolism Male Osteoclasts - drug effects Osteoclasts - enzymology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!