Identification of Caveolin-1 as a Fatty Acid Binding Protein
In an attempt to identify high affinity, fatty acid binding proteins present in 3T3-L1 adipocytes plasma membranes, we labeled proteins in purified plasma membranes with the photoreactive fatty acid analogue, 11-m-diazirinophenoxy[11-3H]undecanoate. A single membrane protein of 22 kDa was covalently...
Gespeichert in:
| Veröffentlicht in: | Biochemical and biophysical research communications 1999-02, Vol.255 (1), p.34-39 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 39 |
|---|---|
| container_issue | 1 |
| container_start_page | 34 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 255 |
| creator | Trigatti, Bernardo L. Anderson, Richard G.W. Gerber, Gerhard E. |
| description | In an attempt to identify high affinity, fatty acid binding proteins present in 3T3-L1 adipocytes plasma membranes, we labeled proteins in purified plasma membranes with the photoreactive fatty acid analogue, 11-m-diazirinophenoxy[11-3H]undecanoate. A single membrane protein of 22 kDa was covalently labeled after photolysis. This protein fractionated with caveolin-1 containing caveolae and was immunoprecipitated by an anti-caveolin-1 monoclonal antibody. Furthermore, 2D-PAGE analysis revealed that both the α and β isoforms of caveolin-1 could be labeled by the photoreactive fatty acid upon photolysis, indicating that both bind fatty acids. The saturable binding of the photoreactive fatty acid suggests caveolin-1 has a lipid binding site that may either operate during intracellular lipid traffic or regulate caveolin-1 function. |
| doi_str_mv | 10.1006/bbrc.1998.0123 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69637181</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0006291X98901231</els_id><sourcerecordid>69637181</sourcerecordid><originalsourceid>FETCH-LOGICAL-c406t-d186b5d3677a9db0a334eb177da3608b77bdf008ffec0eb728d3f4a7c9df58ad3</originalsourceid><addsrcrecordid>eNp1kD1PwzAURS0EoqWwMqJMbAl2nNqJxAIVhUqVYACJzfLHMzJK7WKnlfrvSZQOLExvOffqvoPQNcEFwZjdKRV1QZqmLjAp6QmaEtzgvCS4OkVT3BN52ZDPCbpI6RtjQirWnKNJH61LNidTdL8y4DtnnZadCz4LNlvIPYTW-ZxkMmUyW8quO2QP2pns0Xnj_Ff2FkMHzl-iMyvbBFfHO0Mfy6f3xUu-fn1eLR7Wua4w63JDaqbmhjLOZWMUlpRWoAjnRlKGa8W5MrZfZC1oDIqXtaG2klw3xs5raegM3Y692xh-dpA6sXFJQ9tKD2GXBGsY5aQmPViMoI4hpQhWbKPbyHgQBIvBlxh8icGXGHz1gZtj805twPzBR0E9UI8A9P_tHUSRtAOvwbgIuhMmuP-6fwF33HjD</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69637181</pqid></control><display><type>article</type><title>Identification of Caveolin-1 as a Fatty Acid Binding Protein</title><source>MEDLINE</source><source>ScienceDirect Journals (5 years ago - present)</source><creator>Trigatti, Bernardo L. ; Anderson, Richard G.W. ; Gerber, Gerhard E.</creator><creatorcontrib>Trigatti, Bernardo L. ; Anderson, Richard G.W. ; Gerber, Gerhard E.</creatorcontrib><description>In an attempt to identify high affinity, fatty acid binding proteins present in 3T3-L1 adipocytes plasma membranes, we labeled proteins in purified plasma membranes with the photoreactive fatty acid analogue, 11-m-diazirinophenoxy[11-3H]undecanoate. A single membrane protein of 22 kDa was covalently labeled after photolysis. This protein fractionated with caveolin-1 containing caveolae and was immunoprecipitated by an anti-caveolin-1 monoclonal antibody. Furthermore, 2D-PAGE analysis revealed that both the α and β isoforms of caveolin-1 could be labeled by the photoreactive fatty acid upon photolysis, indicating that both bind fatty acids. The saturable binding of the photoreactive fatty acid suggests caveolin-1 has a lipid binding site that may either operate during intracellular lipid traffic or regulate caveolin-1 function.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1006/bbrc.1998.0123</identifier><identifier>PMID: 10082651</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adipocytes ; Animals ; Binding Sites ; Biological Transport ; Caveolin 1 ; Caveolins ; Cell Line ; Cell Membrane - chemistry ; Cell Membrane - metabolism ; Fatty Acids - chemistry ; Fatty Acids - metabolism ; Membrane Proteins - chemistry ; Membrane Proteins - metabolism ; Mice ; Protein Binding</subject><ispartof>Biochemical and biophysical research communications, 1999-02, Vol.255 (1), p.34-39</ispartof><rights>1999 Academic Press</rights><rights>Copyright 1999 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c406t-d186b5d3677a9db0a334eb177da3608b77bdf008ffec0eb728d3f4a7c9df58ad3</citedby><cites>FETCH-LOGICAL-c406t-d186b5d3677a9db0a334eb177da3608b77bdf008ffec0eb728d3f4a7c9df58ad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/bbrc.1998.0123$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10082651$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Trigatti, Bernardo L.</creatorcontrib><creatorcontrib>Anderson, Richard G.W.</creatorcontrib><creatorcontrib>Gerber, Gerhard E.</creatorcontrib><title>Identification of Caveolin-1 as a Fatty Acid Binding Protein</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>In an attempt to identify high affinity, fatty acid binding proteins present in 3T3-L1 adipocytes plasma membranes, we labeled proteins in purified plasma membranes with the photoreactive fatty acid analogue, 11-m-diazirinophenoxy[11-3H]undecanoate. A single membrane protein of 22 kDa was covalently labeled after photolysis. This protein fractionated with caveolin-1 containing caveolae and was immunoprecipitated by an anti-caveolin-1 monoclonal antibody. Furthermore, 2D-PAGE analysis revealed that both the α and β isoforms of caveolin-1 could be labeled by the photoreactive fatty acid upon photolysis, indicating that both bind fatty acids. The saturable binding of the photoreactive fatty acid suggests caveolin-1 has a lipid binding site that may either operate during intracellular lipid traffic or regulate caveolin-1 function.</description><subject>Adipocytes</subject><subject>Animals</subject><subject>Binding Sites</subject><subject>Biological Transport</subject><subject>Caveolin 1</subject><subject>Caveolins</subject><subject>Cell Line</subject><subject>Cell Membrane - chemistry</subject><subject>Cell Membrane - metabolism</subject><subject>Fatty Acids - chemistry</subject><subject>Fatty Acids - metabolism</subject><subject>Membrane Proteins - chemistry</subject><subject>Membrane Proteins - metabolism</subject><subject>Mice</subject><subject>Protein Binding</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kD1PwzAURS0EoqWwMqJMbAl2nNqJxAIVhUqVYACJzfLHMzJK7WKnlfrvSZQOLExvOffqvoPQNcEFwZjdKRV1QZqmLjAp6QmaEtzgvCS4OkVT3BN52ZDPCbpI6RtjQirWnKNJH61LNidTdL8y4DtnnZadCz4LNlvIPYTW-ZxkMmUyW8quO2QP2pns0Xnj_Ff2FkMHzl-iMyvbBFfHO0Mfy6f3xUu-fn1eLR7Wua4w63JDaqbmhjLOZWMUlpRWoAjnRlKGa8W5MrZfZC1oDIqXtaG2klw3xs5raegM3Y692xh-dpA6sXFJQ9tKD2GXBGsY5aQmPViMoI4hpQhWbKPbyHgQBIvBlxh8icGXGHz1gZtj805twPzBR0E9UI8A9P_tHUSRtAOvwbgIuhMmuP-6fwF33HjD</recordid><startdate>19990205</startdate><enddate>19990205</enddate><creator>Trigatti, Bernardo L.</creator><creator>Anderson, Richard G.W.</creator><creator>Gerber, Gerhard E.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990205</creationdate><title>Identification of Caveolin-1 as a Fatty Acid Binding Protein</title><author>Trigatti, Bernardo L. ; Anderson, Richard G.W. ; Gerber, Gerhard E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c406t-d186b5d3677a9db0a334eb177da3608b77bdf008ffec0eb728d3f4a7c9df58ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adipocytes</topic><topic>Animals</topic><topic>Binding Sites</topic><topic>Biological Transport</topic><topic>Caveolin 1</topic><topic>Caveolins</topic><topic>Cell Line</topic><topic>Cell Membrane - chemistry</topic><topic>Cell Membrane - metabolism</topic><topic>Fatty Acids - chemistry</topic><topic>Fatty Acids - metabolism</topic><topic>Membrane Proteins - chemistry</topic><topic>Membrane Proteins - metabolism</topic><topic>Mice</topic><topic>Protein Binding</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Trigatti, Bernardo L.</creatorcontrib><creatorcontrib>Anderson, Richard G.W.</creatorcontrib><creatorcontrib>Gerber, Gerhard E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Trigatti, Bernardo L.</au><au>Anderson, Richard G.W.</au><au>Gerber, Gerhard E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of Caveolin-1 as a Fatty Acid Binding Protein</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1999-02-05</date><risdate>1999</risdate><volume>255</volume><issue>1</issue><spage>34</spage><epage>39</epage><pages>34-39</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>In an attempt to identify high affinity, fatty acid binding proteins present in 3T3-L1 adipocytes plasma membranes, we labeled proteins in purified plasma membranes with the photoreactive fatty acid analogue, 11-m-diazirinophenoxy[11-3H]undecanoate. A single membrane protein of 22 kDa was covalently labeled after photolysis. This protein fractionated with caveolin-1 containing caveolae and was immunoprecipitated by an anti-caveolin-1 monoclonal antibody. Furthermore, 2D-PAGE analysis revealed that both the α and β isoforms of caveolin-1 could be labeled by the photoreactive fatty acid upon photolysis, indicating that both bind fatty acids. The saturable binding of the photoreactive fatty acid suggests caveolin-1 has a lipid binding site that may either operate during intracellular lipid traffic or regulate caveolin-1 function.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>10082651</pmid><doi>10.1006/bbrc.1998.0123</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-291X |
| ispartof | Biochemical and biophysical research communications, 1999-02, Vol.255 (1), p.34-39 |
| issn | 0006-291X 1090-2104 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69637181 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adipocytes Animals Binding Sites Biological Transport Caveolin 1 Caveolins Cell Line Cell Membrane - chemistry Cell Membrane - metabolism Fatty Acids - chemistry Fatty Acids - metabolism Membrane Proteins - chemistry Membrane Proteins - metabolism Mice Protein Binding |
| title | Identification of Caveolin-1 as a Fatty Acid Binding Protein |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T11%3A59%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20of%20Caveolin-1%20as%20a%20Fatty%20Acid%20Binding%20Protein&rft.jtitle=Biochemical%20and%20biophysical%20research%20communications&rft.au=Trigatti,%20Bernardo%20L.&rft.date=1999-02-05&rft.volume=255&rft.issue=1&rft.spage=34&rft.epage=39&rft.pages=34-39&rft.issn=0006-291X&rft.eissn=1090-2104&rft_id=info:doi/10.1006/bbrc.1998.0123&rft_dat=%3Cproquest_cross%3E69637181%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=69637181&rft_id=info:pmid/10082651&rft_els_id=S0006291X98901231&rfr_iscdi=true |