Characterization of a Specific Antibody to the Rat Angiotensin II AT1 Receptor
We raised a polyclonal antibody against a decapeptide corresponding to the carboxyl terminus of the rat angiotensin II AT1 receptor. This antibody was demonstrated to be specific for the rat receptor according to a number of approaches. These included (a) the ultrastructural localization of immunogo...
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| Veröffentlicht in: | The journal of histochemistry and cytochemistry 1999-04, Vol.47 (4), p.507-515 |
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| container_title | The journal of histochemistry and cytochemistry |
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| creator | Giles, Michelle E Fernley, Ross T Nakamura, Yoshio Moeller, Ingrid Aldred, G. Peter Ferraro, Tania Penschow, Jennifer D McKinley, Michael J Oldfield, Brian J |
| description | We raised a polyclonal antibody against a decapeptide corresponding to the carboxyl terminus of the rat angiotensin II AT1 receptor. This antibody was demonstrated to be specific for the rat receptor according to a number of approaches. These included (a) the ultrastructural localization of immunogold-labeled receptor on the surfaces of zona glomerulosa cells in the adrenal cortex, (b) the specific labeling of Chinese hamster ovarian (CHO) cells transfected with AT1 receptors, (c) the identification of a specific band on Western blots, (d) the immunocytochemical co-localization of angiotensin receptors on neurons in the lamina terminalis of the brain shown to be responsive to circulating angiotensin II, as shown by the expression of c-fos, and (e) the correlation between the expression of the mRNA of the AT1 receptor and AT1 receptor immunoreactivity. |
| doi_str_mv | 10.1177/002215549904700409 |
| format | Article |
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Peter</creatorcontrib><creatorcontrib>Ferraro, Tania</creatorcontrib><creatorcontrib>Penschow, Jennifer D</creatorcontrib><creatorcontrib>McKinley, Michael J</creatorcontrib><creatorcontrib>Oldfield, Brian J</creatorcontrib><title>Characterization of a Specific Antibody to the Rat Angiotensin II AT1 Receptor</title><title>The journal of histochemistry and cytochemistry</title><addtitle>J Histochem Cytochem</addtitle><description>We raised a polyclonal antibody against a decapeptide corresponding to the carboxyl terminus of the rat angiotensin II AT1 receptor. This antibody was demonstrated to be specific for the rat receptor according to a number of approaches. 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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; SAGE Complete; Free Full-Text Journals in Chemistry |
| subjects | Adrenal Cortex - metabolism Angiotensin II - metabolism Animals Antibodies - metabolism Blotting, Western Brain - metabolism CHO Cells Cricetinae Immunohistochemistry Proto-Oncogene Proteins c-fos - metabolism Rats Rats, Sprague-Dawley Receptor, Angiotensin, Type 1 Receptor, Angiotensin, Type 2 Receptors, Angiotensin - genetics Receptors, Angiotensin - immunology Receptors, Angiotensin - metabolism RNA, Messenger - metabolism |
| title | Characterization of a Specific Antibody to the Rat Angiotensin II AT1 Receptor |
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