FtmPT2, an N-Prenyltransferase from Aspergillus fumigatus, Catalyses the Last Step in the Biosynthesis of Fumitremorgin B

Last, but not least: The ftmPT2 gene, which was identified in a biosynthetic gene cluster for fumitremorgins in Aspergillus fumigatus, was over‐expressed in Saccharomyces cerevisiae. The purified soluble dimeric His6–FtmPT2 was characterised biochemically. Structure elucidation of the enzymatic product showed that FtmPT2 catalyses the prenylation of 12,13‐dihydroxyfumitremorgin C in the presence of dimethylallyl diphosphate at position N1 of the indole ring and so functions as an N‐prenyltransferase (see scheme).