Immunoreactivity of endothelins and endothelin receptor in the stria vascularis of the mouse cochlea
Immunoreactivities of endothelin-1, endothelin-3, endothelin receptor type A, and Na,K-ATPase were investigated in the stria vascularis of adult male WBB6F 1 +/+ mice and in that of W/W v mutants lacking strial intermediate cells. In the +/+ mice, electron microscopic immunoreactivity for the endoth...
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| Veröffentlicht in: | Hearing research 1999-02, Vol.128 (1), p.135-146 |
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| creator | Fujimura, Takeyuki Furukawa, Hiroshi Doi, Yoshiaki Makishima, Kazumi Fujimoto, Sunao |
| description | Immunoreactivities of endothelin-1, endothelin-3, endothelin receptor type A, and Na,K-ATPase were investigated in the stria vascularis of adult male WBB6F
1 +/+ mice and in that of W/W
v mutants lacking strial intermediate cells. In the +/+ mice, electron microscopic immunoreactivity for the endothelins was seen on the rough endoplasmic reticulum, Golgi apparatus, cytoplasmic vesicles and lysosomes exclusively in the strial intermediate cells by the postembedment method. Immunoreactive endothelin receptor A was localized along the plasma membrane of strial marginal cells of both wild and mutant types although the immunoreactivity of the latter was much less than that of the former by the preembedment method. These findings suggest that the endothelins, which are produced in the strial intermediate cells, may play a role in the maintenance of the stria vascularis function in the +/+ mice. Since the plasma membrane of the marginal cells of the W/W
v mice, which do not generate a high positive endocochlear potential, also showed immunoreactivity for Na,K-ATPase, it seems likely that the endothelins are involved in the activation of sodium pump of the strial marginal cells by mediation of endothelin receptor A. In addition, the role of lysosomes in the crinophagy of the endothelins in the strial intermediate cells is proposed in the +/+ mice. |
| doi_str_mv | 10.1016/S0378-5955(98)00206-8 |
| format | Article |
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1 +/+ mice and in that of W/W
v mutants lacking strial intermediate cells. In the +/+ mice, electron microscopic immunoreactivity for the endothelins was seen on the rough endoplasmic reticulum, Golgi apparatus, cytoplasmic vesicles and lysosomes exclusively in the strial intermediate cells by the postembedment method. Immunoreactive endothelin receptor A was localized along the plasma membrane of strial marginal cells of both wild and mutant types although the immunoreactivity of the latter was much less than that of the former by the preembedment method. These findings suggest that the endothelins, which are produced in the strial intermediate cells, may play a role in the maintenance of the stria vascularis function in the +/+ mice. Since the plasma membrane of the marginal cells of the W/W
v mice, which do not generate a high positive endocochlear potential, also showed immunoreactivity for Na,K-ATPase, it seems likely that the endothelins are involved in the activation of sodium pump of the strial marginal cells by mediation of endothelin receptor A. In addition, the role of lysosomes in the crinophagy of the endothelins in the strial intermediate cells is proposed in the +/+ mice.</description><identifier>ISSN: 0378-5955</identifier><identifier>EISSN: 1878-5891</identifier><identifier>DOI: 10.1016/S0378-5955(98)00206-8</identifier><identifier>PMID: 10082294</identifier><identifier>CODEN: HERED3</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation ; Endothelin ; Endothelin receptor type A ; Endothelins - metabolism ; Fundamental and applied biological sciences. Psychology ; Immunohistochemistry ; Intermediate cell ; Lysosome ; Male ; Mice ; Mice, Mutant Strains ; Microscopy, Immunoelectron ; Na,K-ATPase ; Receptors, Endothelin - metabolism ; Sodium-Potassium-Exchanging ATPase - metabolism ; Stria vascularis ; Stria Vascularis - cytology ; Stria Vascularis - metabolism ; Stria Vascularis - ultrastructure ; Vertebrates: nervous system and sense organs</subject><ispartof>Hearing research, 1999-02, Vol.128 (1), p.135-146</ispartof><rights>1999 Elsevier Science B.V.</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-bcbc951e2059be0a68d97df02b2f0d30c3407fd75a4ff3b611b355eb32056df53</citedby><cites>FETCH-LOGICAL-c456t-bcbc951e2059be0a68d97df02b2f0d30c3407fd75a4ff3b611b355eb32056df53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0378-5955(98)00206-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1752475$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10082294$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fujimura, Takeyuki</creatorcontrib><creatorcontrib>Furukawa, Hiroshi</creatorcontrib><creatorcontrib>Doi, Yoshiaki</creatorcontrib><creatorcontrib>Makishima, Kazumi</creatorcontrib><creatorcontrib>Fujimoto, Sunao</creatorcontrib><title>Immunoreactivity of endothelins and endothelin receptor in the stria vascularis of the mouse cochlea</title><title>Hearing research</title><addtitle>Hear Res</addtitle><description>Immunoreactivities of endothelin-1, endothelin-3, endothelin receptor type A, and Na,K-ATPase were investigated in the stria vascularis of adult male WBB6F
1 +/+ mice and in that of W/W
v mutants lacking strial intermediate cells. In the +/+ mice, electron microscopic immunoreactivity for the endothelins was seen on the rough endoplasmic reticulum, Golgi apparatus, cytoplasmic vesicles and lysosomes exclusively in the strial intermediate cells by the postembedment method. Immunoreactive endothelin receptor A was localized along the plasma membrane of strial marginal cells of both wild and mutant types although the immunoreactivity of the latter was much less than that of the former by the preembedment method. These findings suggest that the endothelins, which are produced in the strial intermediate cells, may play a role in the maintenance of the stria vascularis function in the +/+ mice. Since the plasma membrane of the marginal cells of the W/W
v mice, which do not generate a high positive endocochlear potential, also showed immunoreactivity for Na,K-ATPase, it seems likely that the endothelins are involved in the activation of sodium pump of the strial marginal cells by mediation of endothelin receptor A. In addition, the role of lysosomes in the crinophagy of the endothelins in the strial intermediate cells is proposed in the +/+ mice.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation</subject><subject>Endothelin</subject><subject>Endothelin receptor type A</subject><subject>Endothelins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunohistochemistry</subject><subject>Intermediate cell</subject><subject>Lysosome</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Mutant Strains</subject><subject>Microscopy, Immunoelectron</subject><subject>Na,K-ATPase</subject><subject>Receptors, Endothelin - metabolism</subject><subject>Sodium-Potassium-Exchanging ATPase - metabolism</subject><subject>Stria vascularis</subject><subject>Stria Vascularis - cytology</subject><subject>Stria Vascularis - metabolism</subject><subject>Stria Vascularis - ultrastructure</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0378-5955</issn><issn>1878-5891</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMFu3CAURVHUKpmk_YREXlRVu3D7AGPDKqqipo0UqYuka4ThoVDZZgL2SPn74swoza4r4Olc3tUh5JzCFwq0_XoHvJO1UEJ8UvIzAIO2lkdkQ-U6loq-IZsX5ISc5vwHgAresGNyQgEkY6rZEHczjssUExo7h12Yn6roK5xcnB9wCFOuzORevauEFrdzTFW5l1GV5xRMtTPZLoNJIa_xdT7GJWNlo30Y0Lwjb70ZMr4_nGfk9_X3-6uf9e2vHzdX325r24h2rnvbWyUoMhCqRzCtdKpzHljPPDgOljfQedcJ03jP-5bSnguBPS-B1nnBz8jH_b_bFB8XzLMeQ7Y4DGbC0ke3quWsEbyAYg_aFHNO6PU2hdGkJ01Br3r1s169utNK6me9WpbcxWHB0o_oXqX2Pgvw4QAUI2bwyUw25H9cJ1jTrUUv9xgWG7uASWcbcLLoQhE8axfDf5r8BTQPmJo</recordid><startdate>19990201</startdate><enddate>19990201</enddate><creator>Fujimura, Takeyuki</creator><creator>Furukawa, Hiroshi</creator><creator>Doi, Yoshiaki</creator><creator>Makishima, Kazumi</creator><creator>Fujimoto, Sunao</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>8BM</scope></search><sort><creationdate>19990201</creationdate><title>Immunoreactivity of endothelins and endothelin receptor in the stria vascularis of the mouse cochlea</title><author>Fujimura, Takeyuki ; Furukawa, Hiroshi ; Doi, Yoshiaki ; Makishima, Kazumi ; Fujimoto, Sunao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-bcbc951e2059be0a68d97df02b2f0d30c3407fd75a4ff3b611b355eb32056df53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation</topic><topic>Endothelin</topic><topic>Endothelin receptor type A</topic><topic>Endothelins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunohistochemistry</topic><topic>Intermediate cell</topic><topic>Lysosome</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Mutant Strains</topic><topic>Microscopy, Immunoelectron</topic><topic>Na,K-ATPase</topic><topic>Receptors, Endothelin - metabolism</topic><topic>Sodium-Potassium-Exchanging ATPase - metabolism</topic><topic>Stria vascularis</topic><topic>Stria Vascularis - cytology</topic><topic>Stria Vascularis - metabolism</topic><topic>Stria Vascularis - ultrastructure</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fujimura, Takeyuki</creatorcontrib><creatorcontrib>Furukawa, Hiroshi</creatorcontrib><creatorcontrib>Doi, Yoshiaki</creatorcontrib><creatorcontrib>Makishima, Kazumi</creatorcontrib><creatorcontrib>Fujimoto, Sunao</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>ComDisDome</collection><jtitle>Hearing research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fujimura, Takeyuki</au><au>Furukawa, Hiroshi</au><au>Doi, Yoshiaki</au><au>Makishima, Kazumi</au><au>Fujimoto, Sunao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunoreactivity of endothelins and endothelin receptor in the stria vascularis of the mouse cochlea</atitle><jtitle>Hearing research</jtitle><addtitle>Hear Res</addtitle><date>1999-02-01</date><risdate>1999</risdate><volume>128</volume><issue>1</issue><spage>135</spage><epage>146</epage><pages>135-146</pages><issn>0378-5955</issn><eissn>1878-5891</eissn><coden>HERED3</coden><abstract>Immunoreactivities of endothelin-1, endothelin-3, endothelin receptor type A, and Na,K-ATPase were investigated in the stria vascularis of adult male WBB6F
1 +/+ mice and in that of W/W
v mutants lacking strial intermediate cells. In the +/+ mice, electron microscopic immunoreactivity for the endothelins was seen on the rough endoplasmic reticulum, Golgi apparatus, cytoplasmic vesicles and lysosomes exclusively in the strial intermediate cells by the postembedment method. Immunoreactive endothelin receptor A was localized along the plasma membrane of strial marginal cells of both wild and mutant types although the immunoreactivity of the latter was much less than that of the former by the preembedment method. These findings suggest that the endothelins, which are produced in the strial intermediate cells, may play a role in the maintenance of the stria vascularis function in the +/+ mice. Since the plasma membrane of the marginal cells of the W/W
v mice, which do not generate a high positive endocochlear potential, also showed immunoreactivity for Na,K-ATPase, it seems likely that the endothelins are involved in the activation of sodium pump of the strial marginal cells by mediation of endothelin receptor A. In addition, the role of lysosomes in the crinophagy of the endothelins in the strial intermediate cells is proposed in the +/+ mice.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>10082294</pmid><doi>10.1016/S0378-5955(98)00206-8</doi><tpages>12</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Ear and associated structures. Auditory pathways and centers. Hearing. Vocal organ. Phonation. Sound production. Echolocation Endothelin Endothelin receptor type A Endothelins - metabolism Fundamental and applied biological sciences. Psychology Immunohistochemistry Intermediate cell Lysosome Male Mice Mice, Mutant Strains Microscopy, Immunoelectron Na,K-ATPase Receptors, Endothelin - metabolism Sodium-Potassium-Exchanging ATPase - metabolism Stria vascularis Stria Vascularis - cytology Stria Vascularis - metabolism Stria Vascularis - ultrastructure Vertebrates: nervous system and sense organs |
| title | Immunoreactivity of endothelins and endothelin receptor in the stria vascularis of the mouse cochlea |
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