Recombinant human thyroid peroxidase expressed in insect cells is soluble at high concentrations and forms diffracting crystals

Human thyroid peroxidase (TPO), the key enzyme in thyroid hormone synthesis, can be produced in active form in the High Five insect cell line and when purified from the cell culture medium is soluble at concentrations of up to 18 mg/ml. This contrasts to a recent report in which human TPO produced i...

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Veröffentlicht in:	Journal of endocrinology 1999-03, Vol.160 (3), p.R13-R15
Hauptverfasser:	Hendry, E, Taylor, G, Ziemnicka, K, Grennan Jones, F, Furmaniak, J, Rees Smith, B
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Cell Line Crystallization Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Humans Insecta Iodide Peroxidase - chemistry Oxidoreductases Recombinant Proteins - chemistry Solubility X-Ray Diffraction
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