Antiprotozoan activity of Brazilian marine cnidarian extracts and of a modified steroid from the octocoral Carijoa riisei
In the present investigation, we have evaluated the antileishmanial and antitrypanosomal activity of methanolic crude extracts obtained from eight species of cnidarians and of a modified steroid isolated from the octocoral Carijoa riisei. The antileishmanial activity of cnidarians crude extracts sho...
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| Veröffentlicht in: | Parasitology research (1987) 2008-11, Vol.103 (6), p.1445-1450 |
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| container_title | Parasitology research (1987) |
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| creator | Reimão, Juliana Quero Migotto, Alvaro Esteves Kossuga, Miriam H Berlinck, Roberto G. S Tempone, André Gustavo |
| description | In the present investigation, we have evaluated the antileishmanial and antitrypanosomal activity of methanolic crude extracts obtained from eight species of cnidarians and of a modified steroid isolated from the octocoral Carijoa riisei. The antileishmanial activity of cnidarians crude extracts showed 50% inhibitory concentration (IC₅₀) values in the concentration range between 2.8 and 93.3 μg/mL. Trypomastigotes of Trypanosoma cruzi were less susceptible to the crude extracts, with IC₅₀ values in the concentration range between 40.9 and 117.9 μg/mL. The steroid (18-acetoxipregna-1,4,20-trien-3-one) displayed a strong antileishmanial activity, with an IC₅₀ value of 5.5 μg/mL against promastigotes and 16.88 μg/mL against intracellular amastigotes. The steroid also displayed mammalian cytotoxicity (IC₅₀ of 10.6 μg/mL), but no hemolytic activity was observed at the highest concentration of 12.5 μg/mL. The antileishmanial effect of the steroid in macrophages suggested other mechanism than macrophage activation, as no upregulation of nitric oxide was observed. The antitrypanosomal activity of the steroid resulted in an IC₅₀ value of 50.5 μg/mL. These results indicate the potential of cnidarian natural compounds as antileishmanial drug candidates. |
| doi_str_mv | 10.1007/s00436-008-1154-6 |
| format | Article |
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The steroid also displayed mammalian cytotoxicity (IC₅₀ of 10.6 μg/mL), but no hemolytic activity was observed at the highest concentration of 12.5 μg/mL. The antileishmanial effect of the steroid in macrophages suggested other mechanism than macrophage activation, as no upregulation of nitric oxide was observed. The antitrypanosomal activity of the steroid resulted in an IC₅₀ value of 50.5 μg/mL. These results indicate the potential of cnidarian natural compounds as antileishmanial drug candidates.</description><identifier>ISSN: 0932-0113</identifier><identifier>EISSN: 1432-1955</identifier><identifier>DOI: 10.1007/s00436-008-1154-6</identifier><identifier>PMID: 18762984</identifier><identifier>CODEN: PARREZ</identifier><language>eng</language><publisher>Berlin/Heidelberg: Berlin/Heidelberg : Springer-Verlag</publisher><subject>Animal and plant ecology ; Animal, plant and microbial ecology ; Animals ; Anthozoa - chemistry ; Antiprotozoal Agents - pharmacology ; Biological and medical sciences ; Biomedical and Life Sciences ; Biomedicine ; Brazil ; Cell Line ; Cnidaria - chemistry ; Cricetinae ; Fundamental and applied biological sciences. Psychology ; General aspects ; General aspects and techniques. Study of several systematic groups. Models ; Immunology ; Invertebrates ; Leishmania - classification ; Leishmania - drug effects ; Leishmania - growth & development ; Medical Microbiology ; Mesocricetus ; Mice ; Mice, Inbred BALB C ; Microbiology ; Original Paper ; Parasitic Sensitivity Tests ; Sea water ecosystems ; Seawater ; Steroids - pharmacology ; Synecology ; Trypanocidal Agents - pharmacology ; Trypanosoma cruzi - drug effects</subject><ispartof>Parasitology research (1987), 2008-11, Vol.103 (6), p.1445-1450</ispartof><rights>Springer-Verlag 2008</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c396t-8dc7c21eafa475b3b4ff772cff97d4d72df04bfb7b49e499e95f035caef0bc213</citedby><cites>FETCH-LOGICAL-c396t-8dc7c21eafa475b3b4ff772cff97d4d72df04bfb7b49e499e95f035caef0bc213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00436-008-1154-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00436-008-1154-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20697051$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18762984$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Reimão, Juliana Quero</creatorcontrib><creatorcontrib>Migotto, Alvaro Esteves</creatorcontrib><creatorcontrib>Kossuga, Miriam H</creatorcontrib><creatorcontrib>Berlinck, Roberto G. S</creatorcontrib><creatorcontrib>Tempone, André Gustavo</creatorcontrib><title>Antiprotozoan activity of Brazilian marine cnidarian extracts and of a modified steroid from the octocoral Carijoa riisei</title><title>Parasitology research (1987)</title><addtitle>Parasitol Res</addtitle><addtitle>Parasitol Res</addtitle><description>In the present investigation, we have evaluated the antileishmanial and antitrypanosomal activity of methanolic crude extracts obtained from eight species of cnidarians and of a modified steroid isolated from the octocoral Carijoa riisei. The antileishmanial activity of cnidarians crude extracts showed 50% inhibitory concentration (IC₅₀) values in the concentration range between 2.8 and 93.3 μg/mL. Trypomastigotes of Trypanosoma cruzi were less susceptible to the crude extracts, with IC₅₀ values in the concentration range between 40.9 and 117.9 μg/mL. The steroid (18-acetoxipregna-1,4,20-trien-3-one) displayed a strong antileishmanial activity, with an IC₅₀ value of 5.5 μg/mL against promastigotes and 16.88 μg/mL against intracellular amastigotes. The steroid also displayed mammalian cytotoxicity (IC₅₀ of 10.6 μg/mL), but no hemolytic activity was observed at the highest concentration of 12.5 μg/mL. The antileishmanial effect of the steroid in macrophages suggested other mechanism than macrophage activation, as no upregulation of nitric oxide was observed. The antitrypanosomal activity of the steroid resulted in an IC₅₀ value of 50.5 μg/mL. These results indicate the potential of cnidarian natural compounds as antileishmanial drug candidates.</description><subject>Animal and plant ecology</subject><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Anthozoa - chemistry</subject><subject>Antiprotozoal Agents - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Brazil</subject><subject>Cell Line</subject><subject>Cnidaria - chemistry</subject><subject>Cricetinae</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>General aspects and techniques. Study of several systematic groups. Models</subject><subject>Immunology</subject><subject>Invertebrates</subject><subject>Leishmania - classification</subject><subject>Leishmania - drug effects</subject><subject>Leishmania - growth & development</subject><subject>Medical Microbiology</subject><subject>Mesocricetus</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>Original Paper</subject><subject>Parasitic Sensitivity Tests</subject><subject>Sea water ecosystems</subject><subject>Seawater</subject><subject>Steroids - pharmacology</subject><subject>Synecology</subject><subject>Trypanocidal Agents - pharmacology</subject><subject>Trypanosoma cruzi - drug effects</subject><issn>0932-0113</issn><issn>1432-1955</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1vFSEUhomxsdfqD3CjbHQ37YFhYFi2N34lTVzUrgnDR-VmBipwjbe_Xm7mRneuOOE874HzIPSGwCUBEFcFgPW8Axg7QgbW8WdoQ1hPOyKH4TnagGw1ENKfo5el7ACI4Iy9QOdkFJzKkW3Q4TrW8JhTTU9JR6xNDb9CPeDk8U3WT2EO7XbROUSHTQy2Ve3C_a65oQXraI-oxkuywQdncakup2Cxz2nB9YfDydRkUtYz3rbwLmmcQyguvEJnXs_FvT6dF-j-08fv2y_d7bfPX7fXt53pJa_daI0wlDjtNRPD1E_MeyGo8V4Ky6yg1gOb_CQmJh2T0snBQz8Y7TxMLdhfoA_r3Lblz70rVS2hGDfPOrq0L4pLTgWVQwPJCpqcSsnOq8cc2uoHRUAdfavVt2q-1dG34i3z9jR8Py3O_kucBDfg_QnQxejZZx1NKH85ClwKGI6_pCtXWis-uKx2aZ9jE_Pf19-tIa-T0g-5Db6_o0B6aP2xyej_AJv6pGA</recordid><startdate>20081101</startdate><enddate>20081101</enddate><creator>Reimão, Juliana Quero</creator><creator>Migotto, Alvaro Esteves</creator><creator>Kossuga, Miriam H</creator><creator>Berlinck, Roberto G. S</creator><creator>Tempone, André Gustavo</creator><general>Berlin/Heidelberg : Springer-Verlag</general><general>Springer-Verlag</general><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20081101</creationdate><title>Antiprotozoan activity of Brazilian marine cnidarian extracts and of a modified steroid from the octocoral Carijoa riisei</title><author>Reimão, Juliana Quero ; Migotto, Alvaro Esteves ; Kossuga, Miriam H ; Berlinck, Roberto G. 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Models</topic><topic>Immunology</topic><topic>Invertebrates</topic><topic>Leishmania - classification</topic><topic>Leishmania - drug effects</topic><topic>Leishmania - growth & development</topic><topic>Medical Microbiology</topic><topic>Mesocricetus</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Original Paper</topic><topic>Parasitic Sensitivity Tests</topic><topic>Sea water ecosystems</topic><topic>Seawater</topic><topic>Steroids - pharmacology</topic><topic>Synecology</topic><topic>Trypanocidal Agents - pharmacology</topic><topic>Trypanosoma cruzi - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reimão, Juliana Quero</creatorcontrib><creatorcontrib>Migotto, Alvaro Esteves</creatorcontrib><creatorcontrib>Kossuga, Miriam H</creatorcontrib><creatorcontrib>Berlinck, Roberto G. S</creatorcontrib><creatorcontrib>Tempone, André Gustavo</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Parasitology research (1987)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reimão, Juliana Quero</au><au>Migotto, Alvaro Esteves</au><au>Kossuga, Miriam H</au><au>Berlinck, Roberto G. S</au><au>Tempone, André Gustavo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antiprotozoan activity of Brazilian marine cnidarian extracts and of a modified steroid from the octocoral Carijoa riisei</atitle><jtitle>Parasitology research (1987)</jtitle><stitle>Parasitol Res</stitle><addtitle>Parasitol Res</addtitle><date>2008-11-01</date><risdate>2008</risdate><volume>103</volume><issue>6</issue><spage>1445</spage><epage>1450</epage><pages>1445-1450</pages><issn>0932-0113</issn><eissn>1432-1955</eissn><coden>PARREZ</coden><abstract>In the present investigation, we have evaluated the antileishmanial and antitrypanosomal activity of methanolic crude extracts obtained from eight species of cnidarians and of a modified steroid isolated from the octocoral Carijoa riisei. The antileishmanial activity of cnidarians crude extracts showed 50% inhibitory concentration (IC₅₀) values in the concentration range between 2.8 and 93.3 μg/mL. Trypomastigotes of Trypanosoma cruzi were less susceptible to the crude extracts, with IC₅₀ values in the concentration range between 40.9 and 117.9 μg/mL. The steroid (18-acetoxipregna-1,4,20-trien-3-one) displayed a strong antileishmanial activity, with an IC₅₀ value of 5.5 μg/mL against promastigotes and 16.88 μg/mL against intracellular amastigotes. The steroid also displayed mammalian cytotoxicity (IC₅₀ of 10.6 μg/mL), but no hemolytic activity was observed at the highest concentration of 12.5 μg/mL. The antileishmanial effect of the steroid in macrophages suggested other mechanism than macrophage activation, as no upregulation of nitric oxide was observed. The antitrypanosomal activity of the steroid resulted in an IC₅₀ value of 50.5 μg/mL. These results indicate the potential of cnidarian natural compounds as antileishmanial drug candidates.</abstract><cop>Berlin/Heidelberg</cop><pub>Berlin/Heidelberg : Springer-Verlag</pub><pmid>18762984</pmid><doi>10.1007/s00436-008-1154-6</doi><tpages>6</tpages></addata></record> |
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| subjects | Animal and plant ecology Animal, plant and microbial ecology Animals Anthozoa - chemistry Antiprotozoal Agents - pharmacology Biological and medical sciences Biomedical and Life Sciences Biomedicine Brazil Cell Line Cnidaria - chemistry Cricetinae Fundamental and applied biological sciences. Psychology General aspects General aspects and techniques. Study of several systematic groups. Models Immunology Invertebrates Leishmania - classification Leishmania - drug effects Leishmania - growth & development Medical Microbiology Mesocricetus Mice Mice, Inbred BALB C Microbiology Original Paper Parasitic Sensitivity Tests Sea water ecosystems Seawater Steroids - pharmacology Synecology Trypanocidal Agents - pharmacology Trypanosoma cruzi - drug effects |
| title | Antiprotozoan activity of Brazilian marine cnidarian extracts and of a modified steroid from the octocoral Carijoa riisei |
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