Corneal Epithelium-specific Mouse Keratin K12 Promoter
Keratins are structural proteins expressed by epithelial cells. Approximately 30 different keratin proteins have been identified, each with a specific expression pattern in different epithelial cells. The tissue-specific promoter of several keratin genes have been used to direct the expression of tr...
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| Veröffentlicht in: | Experimental eye research 1999-03, Vol.68 (3), p.295-301 |
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| creator | LIU, JANICE J. KAO, WINSTON W-Y. WILSON, STEVEN E. |
| description | Keratins are structural proteins expressed by epithelial cells. Approximately 30 different keratin proteins have been identified, each with a specific expression pattern in different epithelial cells. The tissue-specific promoter of several keratin genes have been used to direct the expression of transgenes in animals. Keratin K12 and K3 are expressed in differentiated and stratified corneal epithelium, although the relative expression of each appears to vary between species. We targeted the mouse K12 keratin gene in order to develop a tissue-specific promoter that could be utilized to study the functions of genes of interest expressed in the corneal epithelium. Three 5′ truncated fragments of the keratin K12 promoter (1.03, 0.71 and 0.25 Kb) showed higher functional and tissue-specific promoter activity in a human corneal epithelial cell line than other cell lines. The 0.25 Kb K12 promoter fragment was also active in cultured rabbit corneal epithelial cells. Thus, increased expression in corneal epithelial cells directed by fragments of the mouse K12 promoter extended across species lines. The paired box homeotic gene 6 (PAX-6), which is involved in controlling eye development, stimulated the activity of keratin K12 promoter. |
| doi_str_mv | 10.1006/exer.1998.0593 |
| format | Article |
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Approximately 30 different keratin proteins have been identified, each with a specific expression pattern in different epithelial cells. The tissue-specific promoter of several keratin genes have been used to direct the expression of transgenes in animals. Keratin K12 and K3 are expressed in differentiated and stratified corneal epithelium, although the relative expression of each appears to vary between species. We targeted the mouse K12 keratin gene in order to develop a tissue-specific promoter that could be utilized to study the functions of genes of interest expressed in the corneal epithelium. Three 5′ truncated fragments of the keratin K12 promoter (1.03, 0.71 and 0.25 Kb) showed higher functional and tissue-specific promoter activity in a human corneal epithelial cell line than other cell lines. The 0.25 Kb K12 promoter fragment was also active in cultured rabbit corneal epithelial cells. Thus, increased expression in corneal epithelial cells directed by fragments of the mouse K12 promoter extended across species lines. The paired box homeotic gene 6 (PAX-6), which is involved in controlling eye development, stimulated the activity of keratin K12 promoter.</description><identifier>ISSN: 0014-4835</identifier><identifier>EISSN: 1096-0007</identifier><identifier>DOI: 10.1006/exer.1998.0593</identifier><identifier>PMID: 10079137</identifier><identifier>CODEN: EXERA6</identifier><language>eng</language><publisher>London: Elsevier Ltd</publisher><subject>Animals ; Base Sequence ; Biological and medical sciences ; Cell Line ; Cells, Cultured ; Cloning, Molecular ; corneal epithelium ; DNA-Binding Proteins - genetics ; Epithelium, Corneal - metabolism ; Eye and associated structures. Visual pathways and centers. Vision ; Eye Proteins - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Genes, Homeobox ; Homeodomain Proteins ; Humans ; keratin K12 ; Keratins - genetics ; Mice ; Molecular Sequence Data ; nucleic acid sequence ; Paired Box Transcription Factors ; PAX6 Transcription Factor ; Polymerase Chain Reaction ; promoter ; Promoter Regions, Genetic ; Rabbits ; Repressor Proteins ; Transfection ; Vertebrates: nervous system and sense organs</subject><ispartof>Experimental eye research, 1999-03, Vol.68 (3), p.295-301</ispartof><rights>1999 Academic Press</rights><rights>1999 INIST-CNRS</rights><rights>Copyright 1999 Academic Press.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c369t-c65aeef498581967de58a57b9f486758f8872defa5a92607c38416363572908c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/exer.1998.0593$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1767996$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10079137$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LIU, JANICE J.</creatorcontrib><creatorcontrib>KAO, WINSTON W-Y.</creatorcontrib><creatorcontrib>WILSON, STEVEN E.</creatorcontrib><title>Corneal Epithelium-specific Mouse Keratin K12 Promoter</title><title>Experimental eye research</title><addtitle>Exp Eye Res</addtitle><description>Keratins are structural proteins expressed by epithelial cells. Approximately 30 different keratin proteins have been identified, each with a specific expression pattern in different epithelial cells. The tissue-specific promoter of several keratin genes have been used to direct the expression of transgenes in animals. Keratin K12 and K3 are expressed in differentiated and stratified corneal epithelium, although the relative expression of each appears to vary between species. We targeted the mouse K12 keratin gene in order to develop a tissue-specific promoter that could be utilized to study the functions of genes of interest expressed in the corneal epithelium. Three 5′ truncated fragments of the keratin K12 promoter (1.03, 0.71 and 0.25 Kb) showed higher functional and tissue-specific promoter activity in a human corneal epithelial cell line than other cell lines. The 0.25 Kb K12 promoter fragment was also active in cultured rabbit corneal epithelial cells. Thus, increased expression in corneal epithelial cells directed by fragments of the mouse K12 promoter extended across species lines. The paired box homeotic gene 6 (PAX-6), which is involved in controlling eye development, stimulated the activity of keratin K12 promoter.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Cells, Cultured</subject><subject>Cloning, Molecular</subject><subject>corneal epithelium</subject><subject>DNA-Binding Proteins - genetics</subject><subject>Epithelium, Corneal - metabolism</subject><subject>Eye and associated structures. Visual pathways and centers. Vision</subject><subject>Eye Proteins - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Genes, Homeobox</subject><subject>Homeodomain Proteins</subject><subject>Humans</subject><subject>keratin K12</subject><subject>Keratins - genetics</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>nucleic acid sequence</subject><subject>Paired Box Transcription Factors</subject><subject>PAX6 Transcription Factor</subject><subject>Polymerase Chain Reaction</subject><subject>promoter</subject><subject>Promoter Regions, Genetic</subject><subject>Rabbits</subject><subject>Repressor Proteins</subject><subject>Transfection</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0014-4835</issn><issn>1096-0007</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEtLxDAURoMozji6dSldiLvWpGleSxnGBzOiC12HTHqLkb5MOqL_3pQO6MbVhcv5vns5CJ0TnBGM-TV8gc-IUjLDTNEDNCdY8RRjLA7RHGNSpIWkbIZOQniPW1qI4hjNYlQoQsUc8WXnWzB1surd8Aa12zVp6MG6ytnksdsFSNbgzeDaZE3y5Nl3TTeAP0VHlakDnO3nAr3erl6W9-nm6e5hebNJLeVqSC1nBqAqlGSSKC5KYNIwsVVVIblgspJS5CVUhhmVcywslQXhlFMmcoWlpQt0NfX2vvvYQRh044KFujYtxOc0VzwXuMARzCbQ-i4ED5XuvWuM_9YE69GUHk3p0ZQeTcXAxb55t22g_INPaiJwuQdMsKauvGmtC7-c4EIpHjE5YRA1fLp4I1gHrYXSebCDLjv33ws_o8CCSQ</recordid><startdate>19990301</startdate><enddate>19990301</enddate><creator>LIU, JANICE J.</creator><creator>KAO, WINSTON W-Y.</creator><creator>WILSON, STEVEN E.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990301</creationdate><title>Corneal Epithelium-specific Mouse Keratin K12 Promoter</title><author>LIU, JANICE J. ; KAO, WINSTON W-Y. ; WILSON, STEVEN E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c369t-c65aeef498581967de58a57b9f486758f8872defa5a92607c38416363572908c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Cells, Cultured</topic><topic>Cloning, Molecular</topic><topic>corneal epithelium</topic><topic>DNA-Binding Proteins - genetics</topic><topic>Epithelium, Corneal - metabolism</topic><topic>Eye and associated structures. Visual pathways and centers. Vision</topic><topic>Eye Proteins - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Genes, Homeobox</topic><topic>Homeodomain Proteins</topic><topic>Humans</topic><topic>keratin K12</topic><topic>Keratins - genetics</topic><topic>Mice</topic><topic>Molecular Sequence Data</topic><topic>nucleic acid sequence</topic><topic>Paired Box Transcription Factors</topic><topic>PAX6 Transcription Factor</topic><topic>Polymerase Chain Reaction</topic><topic>promoter</topic><topic>Promoter Regions, Genetic</topic><topic>Rabbits</topic><topic>Repressor Proteins</topic><topic>Transfection</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LIU, JANICE J.</creatorcontrib><creatorcontrib>KAO, WINSTON W-Y.</creatorcontrib><creatorcontrib>WILSON, STEVEN E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental eye research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LIU, JANICE J.</au><au>KAO, WINSTON W-Y.</au><au>WILSON, STEVEN E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Corneal Epithelium-specific Mouse Keratin K12 Promoter</atitle><jtitle>Experimental eye research</jtitle><addtitle>Exp Eye Res</addtitle><date>1999-03-01</date><risdate>1999</risdate><volume>68</volume><issue>3</issue><spage>295</spage><epage>301</epage><pages>295-301</pages><issn>0014-4835</issn><eissn>1096-0007</eissn><coden>EXERA6</coden><abstract>Keratins are structural proteins expressed by epithelial cells. Approximately 30 different keratin proteins have been identified, each with a specific expression pattern in different epithelial cells. The tissue-specific promoter of several keratin genes have been used to direct the expression of transgenes in animals. Keratin K12 and K3 are expressed in differentiated and stratified corneal epithelium, although the relative expression of each appears to vary between species. We targeted the mouse K12 keratin gene in order to develop a tissue-specific promoter that could be utilized to study the functions of genes of interest expressed in the corneal epithelium. Three 5′ truncated fragments of the keratin K12 promoter (1.03, 0.71 and 0.25 Kb) showed higher functional and tissue-specific promoter activity in a human corneal epithelial cell line than other cell lines. The 0.25 Kb K12 promoter fragment was also active in cultured rabbit corneal epithelial cells. Thus, increased expression in corneal epithelial cells directed by fragments of the mouse K12 promoter extended across species lines. The paired box homeotic gene 6 (PAX-6), which is involved in controlling eye development, stimulated the activity of keratin K12 promoter.</abstract><cop>London</cop><pub>Elsevier Ltd</pub><pmid>10079137</pmid><doi>10.1006/exer.1998.0593</doi><tpages>7</tpages></addata></record> |
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| ispartof | Experimental eye research, 1999-03, Vol.68 (3), p.295-301 |
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| subjects | Animals Base Sequence Biological and medical sciences Cell Line Cells, Cultured Cloning, Molecular corneal epithelium DNA-Binding Proteins - genetics Epithelium, Corneal - metabolism Eye and associated structures. Visual pathways and centers. Vision Eye Proteins - genetics Fundamental and applied biological sciences. Psychology Gene Expression Genes, Homeobox Homeodomain Proteins Humans keratin K12 Keratins - genetics Mice Molecular Sequence Data nucleic acid sequence Paired Box Transcription Factors PAX6 Transcription Factor Polymerase Chain Reaction promoter Promoter Regions, Genetic Rabbits Repressor Proteins Transfection Vertebrates: nervous system and sense organs |
| title | Corneal Epithelium-specific Mouse Keratin K12 Promoter |
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