Inhibitory Effects of Hybrid Liposomes on the Growth of Gastric Tumor Cells Established from Cotton Rats
We established cell-line (CoRa 622 G6) of gastric carcinoma using cotton rats with spontaneous malignant gastric carcinoma with hypergastrinaemia. Inhibitory effects of hybrid liposomes (HL) composed of dimyristoylphosphatidylcoline (DMPC) and polyoxyethylene (n) dodecyl ether (C12(EO)n: n=21, 23, 2...
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| Veröffentlicht in: | YAKUGAKU ZASSHI 2008/10/01, Vol.128(10), pp.1485-1492 |
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| container_title | YAKUGAKU ZASSHI |
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| creator | MATSUOKA, Yusuke NAGATA, Miho KOMIZU, Yuji ICHIHARA, Hideaki KAWASE, Shiro SAWADA, Yukiharu MATSUMOTO, Yoko UEOKA, Ryuichi |
| description | We established cell-line (CoRa 622 G6) of gastric carcinoma using cotton rats with spontaneous malignant gastric carcinoma with hypergastrinaemia. Inhibitory effects of hybrid liposomes (HL) composed of dimyristoylphosphatidylcoline (DMPC) and polyoxyethylene (n) dodecyl ether (C12(EO)n: n=21, 23, 25) on the growth of CoRa 622 G6 cells were clarified on the basis of WST-1 assay. Fusion and accumulation of HL including fluorescence probe into CoRa 622 G6 cell membrane were clarified using confocal laser microscopy and total internal reflection fluorescence microscopy. Induction of apoptosis of CoRa 622 G6 cells after the treatment with HL was observed in fluorescence micrographs on the basis of Annexin-V binding assay and TUNEL method using confocal laser microscopy. The results in this study could contribute to the chemotherapy for patients with gastric carcinoma. |
| doi_str_mv | 10.1248/yakushi.128.1485 |
| format | Article |
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Inhibitory effects of hybrid liposomes (HL) composed of dimyristoylphosphatidylcoline (DMPC) and polyoxyethylene (n) dodecyl ether (C12(EO)n: n=21, 23, 25) on the growth of CoRa 622 G6 cells were clarified on the basis of WST-1 assay. Fusion and accumulation of HL including fluorescence probe into CoRa 622 G6 cell membrane were clarified using confocal laser microscopy and total internal reflection fluorescence microscopy. Induction of apoptosis of CoRa 622 G6 cells after the treatment with HL was observed in fluorescence micrographs on the basis of Annexin-V binding assay and TUNEL method using confocal laser microscopy. The results in this study could contribute to the chemotherapy for patients with gastric carcinoma.</description><identifier>ISSN: 0031-6903</identifier><identifier>EISSN: 1347-5231</identifier><identifier>DOI: 10.1248/yakushi.128.1485</identifier><identifier>PMID: 18827469</identifier><language>eng ; jpn</language><publisher>Japan: The Pharmaceutical Society of Japan</publisher><subject>Animals ; apoptosis ; Apoptosis - drug effects ; Cell Division - drug effects ; Cell Line, Tumor ; cotton rat ; Dimyristoylphosphatidylcholine - pharmacology ; Dose-Response Relationship, Drug ; Female ; gastric carcinoma ; hybrid liposome ; Liposomes - pharmacology ; Male ; Microscopy, Confocal ; Sigmodontinae ; Stomach Neoplasms - pathology ; Stomach Neoplasms - ultrastructure</subject><ispartof>YAKUGAKU ZASSHI, 2008/10/01, Vol.128(10), pp.1485-1492</ispartof><rights>2008 by the PHARMACEUTICAL SOCIETY OF JAPAN</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c357t-67f0ce4816e662b0057bd4c071a7745c25063029f92e0bb6b9c00d659dfaf5853</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18827469$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MATSUOKA, Yusuke</creatorcontrib><creatorcontrib>NAGATA, Miho</creatorcontrib><creatorcontrib>KOMIZU, Yuji</creatorcontrib><creatorcontrib>ICHIHARA, Hideaki</creatorcontrib><creatorcontrib>KAWASE, Shiro</creatorcontrib><creatorcontrib>SAWADA, Yukiharu</creatorcontrib><creatorcontrib>MATSUMOTO, Yoko</creatorcontrib><creatorcontrib>UEOKA, Ryuichi</creatorcontrib><title>Inhibitory Effects of Hybrid Liposomes on the Growth of Gastric Tumor Cells Established from Cotton Rats</title><title>YAKUGAKU ZASSHI</title><addtitle>YAKUGAKU ZASSHI</addtitle><description>We established cell-line (CoRa 622 G6) of gastric carcinoma using cotton rats with spontaneous malignant gastric carcinoma with hypergastrinaemia. Inhibitory effects of hybrid liposomes (HL) composed of dimyristoylphosphatidylcoline (DMPC) and polyoxyethylene (n) dodecyl ether (C12(EO)n: n=21, 23, 25) on the growth of CoRa 622 G6 cells were clarified on the basis of WST-1 assay. Fusion and accumulation of HL including fluorescence probe into CoRa 622 G6 cell membrane were clarified using confocal laser microscopy and total internal reflection fluorescence microscopy. Induction of apoptosis of CoRa 622 G6 cells after the treatment with HL was observed in fluorescence micrographs on the basis of Annexin-V binding assay and TUNEL method using confocal laser microscopy. The results in this study could contribute to the chemotherapy for patients with gastric carcinoma.</description><subject>Animals</subject><subject>apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Cell Division - drug effects</subject><subject>Cell Line, Tumor</subject><subject>cotton rat</subject><subject>Dimyristoylphosphatidylcholine - pharmacology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Female</subject><subject>gastric carcinoma</subject><subject>hybrid liposome</subject><subject>Liposomes - pharmacology</subject><subject>Male</subject><subject>Microscopy, Confocal</subject><subject>Sigmodontinae</subject><subject>Stomach Neoplasms - pathology</subject><subject>Stomach Neoplasms - ultrastructure</subject><issn>0031-6903</issn><issn>1347-5231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkM9LwzAYhoMobkzvniQnb9UkbX70KGNuwkAQPZckTWy0XWaSIvvvzdiYmMvHF5735eMB4Aaje0wq8bCTX2PsXF7EPa4EPQNTXFa8oKTE52CKUIkLVqNyAq5jdAohkh_F4hJMsBCEV6yegu550znlkg87uLDW6BSht3C1U8G1cO22PvrB5L8NTJ2By-B_UrcnljKm4DR8Gwcf4Nz0fYSLmKTqXexMC23wA5z7lHLyVaZ4BS6s7KO5Ps4ZeH9avM1Xxfpl-Tx_XBe6pDwVjFukTSUwM4yRfDTlqq004lhyXlFNKGIlIrWtiUFKMVVrhFpG69ZKSwUtZ-Du0LsN_ns0MTWDizqfJzfGj7FhNSOUsyqD6ADq4GMMxjbb4AYZdg1GzV5wcxScF9HsBefI7bF7VINp_wJHnRlYHYDPbOLDnAAZktO9-d-ITiN3nxDdydCYTfkLz1KSRA</recordid><startdate>20081001</startdate><enddate>20081001</enddate><creator>MATSUOKA, Yusuke</creator><creator>NAGATA, Miho</creator><creator>KOMIZU, Yuji</creator><creator>ICHIHARA, Hideaki</creator><creator>KAWASE, Shiro</creator><creator>SAWADA, Yukiharu</creator><creator>MATSUMOTO, Yoko</creator><creator>UEOKA, Ryuichi</creator><general>The Pharmaceutical Society of Japan</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20081001</creationdate><title>Inhibitory Effects of Hybrid Liposomes on the Growth of Gastric Tumor Cells Established from Cotton Rats</title><author>MATSUOKA, Yusuke ; NAGATA, Miho ; KOMIZU, Yuji ; ICHIHARA, Hideaki ; KAWASE, Shiro ; SAWADA, Yukiharu ; MATSUMOTO, Yoko ; UEOKA, Ryuichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-67f0ce4816e662b0057bd4c071a7745c25063029f92e0bb6b9c00d659dfaf5853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng ; jpn</language><creationdate>2008</creationdate><topic>Animals</topic><topic>apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Cell Division - drug effects</topic><topic>Cell Line, Tumor</topic><topic>cotton rat</topic><topic>Dimyristoylphosphatidylcholine - pharmacology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Female</topic><topic>gastric carcinoma</topic><topic>hybrid liposome</topic><topic>Liposomes - pharmacology</topic><topic>Male</topic><topic>Microscopy, Confocal</topic><topic>Sigmodontinae</topic><topic>Stomach Neoplasms - pathology</topic><topic>Stomach Neoplasms - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MATSUOKA, Yusuke</creatorcontrib><creatorcontrib>NAGATA, Miho</creatorcontrib><creatorcontrib>KOMIZU, Yuji</creatorcontrib><creatorcontrib>ICHIHARA, Hideaki</creatorcontrib><creatorcontrib>KAWASE, Shiro</creatorcontrib><creatorcontrib>SAWADA, Yukiharu</creatorcontrib><creatorcontrib>MATSUMOTO, Yoko</creatorcontrib><creatorcontrib>UEOKA, Ryuichi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>YAKUGAKU ZASSHI</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MATSUOKA, Yusuke</au><au>NAGATA, Miho</au><au>KOMIZU, Yuji</au><au>ICHIHARA, Hideaki</au><au>KAWASE, Shiro</au><au>SAWADA, Yukiharu</au><au>MATSUMOTO, Yoko</au><au>UEOKA, Ryuichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibitory Effects of Hybrid Liposomes on the Growth of Gastric Tumor Cells Established from Cotton Rats</atitle><jtitle>YAKUGAKU ZASSHI</jtitle><addtitle>YAKUGAKU ZASSHI</addtitle><date>2008-10-01</date><risdate>2008</risdate><volume>128</volume><issue>10</issue><spage>1485</spage><epage>1492</epage><pages>1485-1492</pages><issn>0031-6903</issn><eissn>1347-5231</eissn><abstract>We established cell-line (CoRa 622 G6) of gastric carcinoma using cotton rats with spontaneous malignant gastric carcinoma with hypergastrinaemia. Inhibitory effects of hybrid liposomes (HL) composed of dimyristoylphosphatidylcoline (DMPC) and polyoxyethylene (n) dodecyl ether (C12(EO)n: n=21, 23, 25) on the growth of CoRa 622 G6 cells were clarified on the basis of WST-1 assay. Fusion and accumulation of HL including fluorescence probe into CoRa 622 G6 cell membrane were clarified using confocal laser microscopy and total internal reflection fluorescence microscopy. Induction of apoptosis of CoRa 622 G6 cells after the treatment with HL was observed in fluorescence micrographs on the basis of Annexin-V binding assay and TUNEL method using confocal laser microscopy. The results in this study could contribute to the chemotherapy for patients with gastric carcinoma.</abstract><cop>Japan</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>18827469</pmid><doi>10.1248/yakushi.128.1485</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | YAKUGAKU ZASSHI, 2008/10/01, Vol.128(10), pp.1485-1492 |
| issn | 0031-6903 1347-5231 |
| language | eng ; jpn |
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| source | J-STAGE Free; MEDLINE; Alma/SFX Local Collection |
| subjects | Animals apoptosis Apoptosis - drug effects Cell Division - drug effects Cell Line, Tumor cotton rat Dimyristoylphosphatidylcholine - pharmacology Dose-Response Relationship, Drug Female gastric carcinoma hybrid liposome Liposomes - pharmacology Male Microscopy, Confocal Sigmodontinae Stomach Neoplasms - pathology Stomach Neoplasms - ultrastructure |
| title | Inhibitory Effects of Hybrid Liposomes on the Growth of Gastric Tumor Cells Established from Cotton Rats |
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