Identification of Bordetella pertussis virulence-associated outer membrane proteins
Abstract Bordetella pertussis virulence-associated 30-, 32-, 90- and 95-kDa outer membrane proteins were purified and their N-terminal amino acid sequences were determined. The 30- and 32-kDa outer membrane proteins showed identity to the C-terminal region of the precursors of the serum resistance p...
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| Veröffentlicht in: | FEMS microbiology letters 1999-03, Vol.172 (1), p.9-13 |
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| container_title | FEMS microbiology letters |
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| creator | Passerini de Rossi, Beatriz N. Friedman, Laura E. González Flecha, F. Luis Castello, Pablo R. Franco, Mirta A. Rossi, Juan Pablo F.C. |
| description | Abstract
Bordetella pertussis virulence-associated 30-, 32-, 90- and 95-kDa outer membrane proteins were purified and their N-terminal amino acid sequences were determined. The 30- and 32-kDa outer membrane proteins showed identity to the C-terminal region of the precursors of the serum resistance protein (BrkA) and the tracheal colonization factor, respectively. We confirmed the cleavage site of these precursors after N731 for BrkA and after N393 for tracheal colonization factor. Associated with the 32-kDa outer membrane protein, we found a new group of 36-kDa virulence-associated peptides. The 95-kDa outer membrane protein showed identity to Vag8. The 90-kDa outer membrane protein did not show homology with the described proteins. We report the N-termini sequence of Vir-90, a novel potential virulence factor. |
| doi_str_mv | 10.1111/j.1574-6968.1999.tb13442.x |
| format | Article |
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Bordetella pertussis virulence-associated 30-, 32-, 90- and 95-kDa outer membrane proteins were purified and their N-terminal amino acid sequences were determined. The 30- and 32-kDa outer membrane proteins showed identity to the C-terminal region of the precursors of the serum resistance protein (BrkA) and the tracheal colonization factor, respectively. We confirmed the cleavage site of these precursors after N731 for BrkA and after N393 for tracheal colonization factor. Associated with the 32-kDa outer membrane protein, we found a new group of 36-kDa virulence-associated peptides. The 95-kDa outer membrane protein showed identity to Vag8. The 90-kDa outer membrane protein did not show homology with the described proteins. We report the N-termini sequence of Vir-90, a novel potential virulence factor.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.1999.tb13442.x</identifier><identifier>PMID: 10079522</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Amino Acid Sequence ; Amino acids ; Bacterial Outer Membrane Proteins - chemistry ; Bacterial Outer Membrane Proteins - isolation & purification ; Bacteriology ; Biological and medical sciences ; Bordetella pertussis ; Bordetella pertussis - chemistry ; Bordetella pertussis - pathogenicity ; BrkA precursor ; Colonization ; Colonization factor ; Electrophoresis, Polyacrylamide Gel - methods ; Fundamental and applied biological sciences. Psychology ; Homology ; Humans ; Membrane proteins ; Membranes ; Microbiology ; Molecular Sequence Data ; Outer membrane proteins ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Peptides ; Pertussis ; Precursors ; Protein Precursors - chemistry ; Proteins ; Sequence Alignment ; Tracheal colonization factor precursor ; Vag8 ; Virulence ; Virulence associated‐outer membrane protein ; Virulence factors ; Vir‐90 ; Whooping cough</subject><ispartof>FEMS microbiology letters, 1999-03, Vol.172 (1), p.9-13</ispartof><rights>1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. 1999</rights><rights>1999 INIST-CNRS</rights><rights>1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4039-a593ffbe316de4e455231167051f5311e3c7755c2e10f2bd3dffe63a2fc494953</citedby><cites>FETCH-LOGICAL-c4039-a593ffbe316de4e455231167051f5311e3c7755c2e10f2bd3dffe63a2fc494953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.1999.tb13442.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.1999.tb13442.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1775409$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10079522$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Passerini de Rossi, Beatriz N.</creatorcontrib><creatorcontrib>Friedman, Laura E.</creatorcontrib><creatorcontrib>González Flecha, F. Luis</creatorcontrib><creatorcontrib>Castello, Pablo R.</creatorcontrib><creatorcontrib>Franco, Mirta A.</creatorcontrib><creatorcontrib>Rossi, Juan Pablo F.C.</creatorcontrib><title>Identification of Bordetella pertussis virulence-associated outer membrane proteins</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
Bordetella pertussis virulence-associated 30-, 32-, 90- and 95-kDa outer membrane proteins were purified and their N-terminal amino acid sequences were determined. The 30- and 32-kDa outer membrane proteins showed identity to the C-terminal region of the precursors of the serum resistance protein (BrkA) and the tracheal colonization factor, respectively. We confirmed the cleavage site of these precursors after N731 for BrkA and after N393 for tracheal colonization factor. Associated with the 32-kDa outer membrane protein, we found a new group of 36-kDa virulence-associated peptides. The 95-kDa outer membrane protein showed identity to Vag8. The 90-kDa outer membrane protein did not show homology with the described proteins. We report the N-termini sequence of Vir-90, a novel potential virulence factor.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Bacterial Outer Membrane Proteins - chemistry</subject><subject>Bacterial Outer Membrane Proteins - isolation & purification</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Bordetella pertussis</subject><subject>Bordetella pertussis - chemistry</subject><subject>Bordetella pertussis - pathogenicity</subject><subject>BrkA precursor</subject><subject>Colonization</subject><subject>Colonization factor</subject><subject>Electrophoresis, Polyacrylamide Gel - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Homology</subject><subject>Humans</subject><subject>Membrane proteins</subject><subject>Membranes</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Outer membrane proteins</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Peptides</subject><subject>Pertussis</subject><subject>Precursors</subject><subject>Protein Precursors - chemistry</subject><subject>Proteins</subject><subject>Sequence Alignment</subject><subject>Tracheal colonization factor precursor</subject><subject>Vag8</subject><subject>Virulence</subject><subject>Virulence associated‐outer membrane protein</subject><subject>Virulence factors</subject><subject>Vir‐90</subject><subject>Whooping cough</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqVkM1u1DAURi0EotPCK6AIELsE_8ZjxKZULVQaxAJYW45zLXmUxIPt0PbtcZQRoKos8CaWfL57vxyEXhLckHLe7hsiJK9b1W4bopRqckcY57S5fYQ2v58eow1mclsTrOQJOk1pjzHmFLdP0QnBWCpB6QZ9ve5hyt55a7IPUxVc9SHEHjIMg6kOEPOckk_VTx_nASYLtUkpWG8y9FWYM8RqhLGLZoLqEEMGP6Vn6IkzQ4Lnx-8Z-n51-e3iU7378vH64nxXW46Zqo1QzLkOGGl74MCFoIyQVmJBnCg3YFZKISwFgh3tetY7By0z1FmuuBLsDL1Z55bFP2ZIWY8-2aX4BGFOuligfCtJAV_dA_dhjlPpppeVUnDZLuPerZSNIaUITh-iH0280wTrRbze68XuMnerF_H6KF7flvCL44q5G6H_K7qaLsDrI2CSNYMrxqxPf7jyqxyrgr1fsRs_wN1_NNBXn3dLXKzxMB_-Ea4fqv8L9P-v0A</recordid><startdate>199903</startdate><enddate>199903</enddate><creator>Passerini de Rossi, Beatriz N.</creator><creator>Friedman, Laura E.</creator><creator>González Flecha, F. Luis</creator><creator>Castello, Pablo R.</creator><creator>Franco, Mirta A.</creator><creator>Rossi, Juan Pablo F.C.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199903</creationdate><title>Identification of Bordetella pertussis virulence-associated outer membrane proteins</title><author>Passerini de Rossi, Beatriz N. ; Friedman, Laura E. ; González Flecha, F. Luis ; Castello, Pablo R. ; Franco, Mirta A. ; Rossi, Juan Pablo F.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4039-a593ffbe316de4e455231167051f5311e3c7755c2e10f2bd3dffe63a2fc494953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Bacterial Outer Membrane Proteins - chemistry</topic><topic>Bacterial Outer Membrane Proteins - isolation & purification</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Bordetella pertussis</topic><topic>Bordetella pertussis - chemistry</topic><topic>Bordetella pertussis - pathogenicity</topic><topic>BrkA precursor</topic><topic>Colonization</topic><topic>Colonization factor</topic><topic>Electrophoresis, Polyacrylamide Gel - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Homology</topic><topic>Humans</topic><topic>Membrane proteins</topic><topic>Membranes</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Outer membrane proteins</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Peptides</topic><topic>Pertussis</topic><topic>Precursors</topic><topic>Protein Precursors - chemistry</topic><topic>Proteins</topic><topic>Sequence Alignment</topic><topic>Tracheal colonization factor precursor</topic><topic>Vag8</topic><topic>Virulence</topic><topic>Virulence associated‐outer membrane protein</topic><topic>Virulence factors</topic><topic>Vir‐90</topic><topic>Whooping cough</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Passerini de Rossi, Beatriz N.</creatorcontrib><creatorcontrib>Friedman, Laura E.</creatorcontrib><creatorcontrib>González Flecha, F. 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Luis</au><au>Castello, Pablo R.</au><au>Franco, Mirta A.</au><au>Rossi, Juan Pablo F.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of Bordetella pertussis virulence-associated outer membrane proteins</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>1999-03</date><risdate>1999</risdate><volume>172</volume><issue>1</issue><spage>9</spage><epage>13</epage><pages>9-13</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Abstract
Bordetella pertussis virulence-associated 30-, 32-, 90- and 95-kDa outer membrane proteins were purified and their N-terminal amino acid sequences were determined. The 30- and 32-kDa outer membrane proteins showed identity to the C-terminal region of the precursors of the serum resistance protein (BrkA) and the tracheal colonization factor, respectively. We confirmed the cleavage site of these precursors after N731 for BrkA and after N393 for tracheal colonization factor. Associated with the 32-kDa outer membrane protein, we found a new group of 36-kDa virulence-associated peptides. The 95-kDa outer membrane protein showed identity to Vag8. The 90-kDa outer membrane protein did not show homology with the described proteins. We report the N-termini sequence of Vir-90, a novel potential virulence factor.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>10079522</pmid><doi>10.1111/j.1574-6968.1999.tb13442.x</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1999-03, Vol.172 (1), p.9-13 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69624871 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Amino acids Bacterial Outer Membrane Proteins - chemistry Bacterial Outer Membrane Proteins - isolation & purification Bacteriology Biological and medical sciences Bordetella pertussis Bordetella pertussis - chemistry Bordetella pertussis - pathogenicity BrkA precursor Colonization Colonization factor Electrophoresis, Polyacrylamide Gel - methods Fundamental and applied biological sciences. Psychology Homology Humans Membrane proteins Membranes Microbiology Molecular Sequence Data Outer membrane proteins Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Peptides Pertussis Precursors Protein Precursors - chemistry Proteins Sequence Alignment Tracheal colonization factor precursor Vag8 Virulence Virulence associated‐outer membrane protein Virulence factors Vir‐90 Whooping cough |
| title | Identification of Bordetella pertussis virulence-associated outer membrane proteins |
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