Reconstitution of a Core Chromatin Remodeling Complex from SWI/SNF Subunits
Protein complexes of the SWI/SNF family remodel nucleosome structure in an ATP-dependent manner. Each complex contains between 8 and 15 subunits, several of which are highly conserved between yeast, Drosophila, and humans. We have reconstituted an ATP-dependent chromatin remodeling complex using a s...
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| Veröffentlicht in: | Molecular cell 1999-02, Vol.3 (2), p.247-253 |
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| creator | Phelan, Michael L Sif, Saïd Narlikar, Geeta J Kingston, Robert E |
| description | Protein complexes of the SWI/SNF family remodel nucleosome structure in an ATP-dependent manner. Each complex contains between 8 and 15 subunits, several of which are highly conserved between yeast,
Drosophila, and humans. We have reconstituted an ATP-dependent chromatin remodeling complex using a subset of conserved subunits. Unexpectedly, both BRG1 and hBRM, the ATPase subunits of human SWI/SNF complexes, are capable of remodeling mononucleosomes and nucleosomal arrays as purified proteins. The addition of INI1, BAF155, and BAF170 to BRG1 increases remodeling activity to a level comparable to that of the whole hSWI/SNF complex. These data define the functional core of the hSWI/SNF complex. |
| doi_str_mv | 10.1016/S1097-2765(00)80315-9 |
| format | Article |
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Drosophila, and humans. We have reconstituted an ATP-dependent chromatin remodeling complex using a subset of conserved subunits. Unexpectedly, both BRG1 and hBRM, the ATPase subunits of human SWI/SNF complexes, are capable of remodeling mononucleosomes and nucleosomal arrays as purified proteins. The addition of INI1, BAF155, and BAF170 to BRG1 increases remodeling activity to a level comparable to that of the whole hSWI/SNF complex. These data define the functional core of the hSWI/SNF complex.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>10078207</pmid><doi>10.1016/S1097-2765(00)80315-9</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Cell Press Free Archives; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Actins - chemistry Actins - physiology Adenosine Triphosphate - physiology Cell-Free System Chromatin - metabolism Chromatin - ultrastructure Chromosomal Proteins, Non-Histone DNA Helicases DNA-Binding Proteins - chemistry DNA-Binding Proteins - physiology Drosophila Proteins Evolution, Molecular Humans Macromolecular Substances Nuclear Proteins - chemistry Nuclear Proteins - physiology Nucleosomes - metabolism Nucleosomes - ultrastructure Recombinant Fusion Proteins - chemistry Ribonucleoprotein, U1 Small Nuclear - chemistry Ribonucleoprotein, U1 Small Nuclear - physiology RNA-Binding Proteins Transcription Factors - chemistry Transcription Factors - physiology |
| title | Reconstitution of a Core Chromatin Remodeling Complex from SWI/SNF Subunits |
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