Techniques for the production of dental eroded lesions in vitro
This project firstly demonstrated an in vitro technique for the production of eroded lesions which simulates the in vivo conditions, and secondly evaluated the influence of salivary parameters on the degree of erosion in vitro. Teeth were sectioned sagitally into three equal portions. The portions w...
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Veröffentlicht in: | Journal of oral rehabilitation 1999-02, Vol.26 (2), p.97-102 |
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creator | Amaechi, B. T. Higham, S. M. Edgar, W. M. |
description | This project firstly demonstrated an in vitro technique for the production of eroded lesions which simulates the in vivo conditions, and secondly evaluated the influence of salivary parameters on the degree of erosion in vitro. Teeth were sectioned sagitally into three equal portions. The portions were randomly assigned to one of three experimental groups (A, B and C). Lesions were produced by cycling the teeth between orange juice and either artificial saliva (A) or water (B) for a total period of 12 h, or by a single 12 h immersion in orange juice (C). Sections were prepared from each specimen and mineral loss was quantified using transverse microradiography. Mineral loss was significantly lower in group A compared with B and C. Greatest mineral loss was observed in B though not significant when compared with C. The present technique mimics the in vivo conditions with the saliva reducing the degree of erosion and possibly remineralizing the lesions. |
doi_str_mv | 10.1046/j.1365-2842.1999.00349.x |
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T. ; Higham, S. M. ; Edgar, W. M.</creator><creatorcontrib>Amaechi, B. T. ; Higham, S. M. ; Edgar, W. M.</creatorcontrib><description>This project firstly demonstrated an in vitro technique for the production of eroded lesions which simulates the in vivo conditions, and secondly evaluated the influence of salivary parameters on the degree of erosion in vitro. Teeth were sectioned sagitally into three equal portions. The portions were randomly assigned to one of three experimental groups (A, B and C). Lesions were produced by cycling the teeth between orange juice and either artificial saliva (A) or water (B) for a total period of 12 h, or by a single 12 h immersion in orange juice (C). Sections were prepared from each specimen and mineral loss was quantified using transverse microradiography. Mineral loss was significantly lower in group A compared with B and C. Greatest mineral loss was observed in B though not significant when compared with C. The present technique mimics the in vivo conditions with the saliva reducing the degree of erosion and possibly remineralizing the lesions.</description><identifier>ISSN: 0305-182X</identifier><identifier>EISSN: 1365-2842</identifier><identifier>DOI: 10.1046/j.1365-2842.1999.00349.x</identifier><identifier>PMID: 10080305</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Animals ; Beverages - adverse effects ; Cattle ; Citrus ; Dental Enamel - chemistry ; Dentistry ; Image Processing, Computer-Assisted ; Immersion ; Microradiography ; Minerals - analysis ; Random Allocation ; Saliva - physiology ; Saliva, Artificial - pharmacology ; Time Factors ; Tooth Erosion - etiology ; Tooth Erosion - metabolism ; Tooth Remineralization ; Water</subject><ispartof>Journal of oral rehabilitation, 1999-02, Vol.26 (2), p.97-102</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4039-692184d0d4cd11c44e2639f172ae4c46c840a779d40e4b1f979323150bfbfa393</citedby><cites>FETCH-LOGICAL-c4039-692184d0d4cd11c44e2639f172ae4c46c840a779d40e4b1f979323150bfbfa393</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2842.1999.00349.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2842.1999.00349.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10080305$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Amaechi, B. T.</creatorcontrib><creatorcontrib>Higham, S. M.</creatorcontrib><creatorcontrib>Edgar, W. M.</creatorcontrib><title>Techniques for the production of dental eroded lesions in vitro</title><title>Journal of oral rehabilitation</title><addtitle>Journal of Oral Rehabilitation</addtitle><description>This project firstly demonstrated an in vitro technique for the production of eroded lesions which simulates the in vivo conditions, and secondly evaluated the influence of salivary parameters on the degree of erosion in vitro. Teeth were sectioned sagitally into three equal portions. The portions were randomly assigned to one of three experimental groups (A, B and C). Lesions were produced by cycling the teeth between orange juice and either artificial saliva (A) or water (B) for a total period of 12 h, or by a single 12 h immersion in orange juice (C). Sections were prepared from each specimen and mineral loss was quantified using transverse microradiography. Mineral loss was significantly lower in group A compared with B and C. Greatest mineral loss was observed in B though not significant when compared with C. The present technique mimics the in vivo conditions with the saliva reducing the degree of erosion and possibly remineralizing the lesions.</description><subject>Animals</subject><subject>Beverages - adverse effects</subject><subject>Cattle</subject><subject>Citrus</subject><subject>Dental Enamel - chemistry</subject><subject>Dentistry</subject><subject>Image Processing, Computer-Assisted</subject><subject>Immersion</subject><subject>Microradiography</subject><subject>Minerals - analysis</subject><subject>Random Allocation</subject><subject>Saliva - physiology</subject><subject>Saliva, Artificial - pharmacology</subject><subject>Time Factors</subject><subject>Tooth Erosion - etiology</subject><subject>Tooth Erosion - metabolism</subject><subject>Tooth Remineralization</subject><subject>Water</subject><issn>0305-182X</issn><issn>1365-2842</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkFFLwzAQx4Mobk6_guTJt9akSdPmQUSGTmVzIJMNX0KbXlln186m1e3bm9oxfPQpx93vfzl-CGFKXEq4uF65lAnf8ULuuVRK6RLCuHS3R6h_GByjPmHEd2joLXrozJgVISRkfnCKetRW7bCPbmegl0X22YDBaVnhegl4U5VJo-usLHCZ4gSKOsox2CYkOAdj-wZnBf7K6qo8RydplBu42L8D9PZwPxs-OuPp6Gl4N3Y0J0w6Qno05AlJuE4o1ZyDJ5hMaeBFwDUXOuQkCgKZcAI8pqkMJPMY9UmcxmnEJBugq26vPa49tlbrzGjI86iAsjFKSGH5UFgw7EBdlcZUkKpNla2jaqcoUa08tVKtI9U6Uq089StPbW30cv9HE68h-RPsbFngpgO-sxx2_16snqfTV1vZvNPlM1PD9pCPqg8lAhb4av4yUmyyEO9zRtWE_QDnfYv3</recordid><startdate>199902</startdate><enddate>199902</enddate><creator>Amaechi, B. T.</creator><creator>Higham, S. M.</creator><creator>Edgar, W. M.</creator><general>Blackwell Science Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199902</creationdate><title>Techniques for the production of dental eroded lesions in vitro</title><author>Amaechi, B. T. ; Higham, S. M. ; Edgar, W. M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4039-692184d0d4cd11c44e2639f172ae4c46c840a779d40e4b1f979323150bfbfa393</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Beverages - adverse effects</topic><topic>Cattle</topic><topic>Citrus</topic><topic>Dental Enamel - chemistry</topic><topic>Dentistry</topic><topic>Image Processing, Computer-Assisted</topic><topic>Immersion</topic><topic>Microradiography</topic><topic>Minerals - analysis</topic><topic>Random Allocation</topic><topic>Saliva - physiology</topic><topic>Saliva, Artificial - pharmacology</topic><topic>Time Factors</topic><topic>Tooth Erosion - etiology</topic><topic>Tooth Erosion - metabolism</topic><topic>Tooth Remineralization</topic><topic>Water</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Amaechi, B. T.</creatorcontrib><creatorcontrib>Higham, S. M.</creatorcontrib><creatorcontrib>Edgar, W. M.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of oral rehabilitation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Amaechi, B. T.</au><au>Higham, S. M.</au><au>Edgar, W. M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Techniques for the production of dental eroded lesions in vitro</atitle><jtitle>Journal of oral rehabilitation</jtitle><addtitle>Journal of Oral Rehabilitation</addtitle><date>1999-02</date><risdate>1999</risdate><volume>26</volume><issue>2</issue><spage>97</spage><epage>102</epage><pages>97-102</pages><issn>0305-182X</issn><eissn>1365-2842</eissn><abstract>This project firstly demonstrated an in vitro technique for the production of eroded lesions which simulates the in vivo conditions, and secondly evaluated the influence of salivary parameters on the degree of erosion in vitro. Teeth were sectioned sagitally into three equal portions. The portions were randomly assigned to one of three experimental groups (A, B and C). Lesions were produced by cycling the teeth between orange juice and either artificial saliva (A) or water (B) for a total period of 12 h, or by a single 12 h immersion in orange juice (C). Sections were prepared from each specimen and mineral loss was quantified using transverse microradiography. Mineral loss was significantly lower in group A compared with B and C. Greatest mineral loss was observed in B though not significant when compared with C. The present technique mimics the in vivo conditions with the saliva reducing the degree of erosion and possibly remineralizing the lesions.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>10080305</pmid><doi>10.1046/j.1365-2842.1999.00349.x</doi><tpages>6</tpages></addata></record> |
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subjects | Animals Beverages - adverse effects Cattle Citrus Dental Enamel - chemistry Dentistry Image Processing, Computer-Assisted Immersion Microradiography Minerals - analysis Random Allocation Saliva - physiology Saliva, Artificial - pharmacology Time Factors Tooth Erosion - etiology Tooth Erosion - metabolism Tooth Remineralization Water |
title | Techniques for the production of dental eroded lesions in vitro |
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