Induction of human cytomegalovirus (HCMV)-glycoprotein B (gB)-specific neutralizing antibody and phosphoprotein 65 (pp65)-specific cytotoxic T lymphocyte responses by naked DNA immunization
Plasmids expressing the human cytomegalovirus (HCMV) glycoprotein B (gB) (UL55) or phosphoprotein 65 (pp65) (UL83) were constructed and evaluated for their ability to induce immune responses in mice. The full-length gB as well as a truncated form expressing amino acids 1–680 of gB, and lacking the f...
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| Veröffentlicht in: | Vaccine 1999, Vol.17 (1), p.50-58 |
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| creator | Endresz, Valeria Kari, Laszlo Berencsi, Klara Kari, Csaba Gyulai, Zsofia Jeney, Csaba Pincus, Steve Rodeck, Ulrich Méric, Claude Plotkin, Stanley A Gönczöl, Eva |
| description | Plasmids expressing the human cytomegalovirus (HCMV) glycoprotein B (gB) (UL55) or phosphoprotein 65 (pp65) (UL83) were constructed and evaluated for their ability to induce immune responses in mice. The full-length gB as well as a truncated form expressing amino acids 1–680 of gB, and lacking the fragment encoding amino acids 681–907 including the transmembrane domain of gB (gB680) were evaluated. Immunization of mice with plasmids coding for gB or gB680 induced ELISA and neutralizing antibodies, with the highest titres in mice immunized with the gB680 plasmid. Mice immunized with the gB plasmid predominantly produced IgG2a gB-specific antibody, while the gB680 plasmid raised mostly IgG1 anti-gB antibody. Mice immunized with the pp65 plasmid developed pp65-specific cytotoxic T lymphocytes (CTL) and ELISA antibodies. Immunization with a mixture of both gB and pp65 plasmids raised antibodies to both proteins and pp65-specific CTL, indicating a lack of interference between these two plasmids. These results suggest that DNA immunization is a useful approach for vaccination against HCMV disease. |
| doi_str_mv | 10.1016/S0264-410X(98)00145-5 |
| format | Article |
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The full-length gB as well as a truncated form expressing amino acids 1–680 of gB, and lacking the fragment encoding amino acids 681–907 including the transmembrane domain of gB (gB680) were evaluated. Immunization of mice with plasmids coding for gB or gB680 induced ELISA and neutralizing antibodies, with the highest titres in mice immunized with the gB680 plasmid. Mice immunized with the gB plasmid predominantly produced IgG2a gB-specific antibody, while the gB680 plasmid raised mostly IgG1 anti-gB antibody. Mice immunized with the pp65 plasmid developed pp65-specific cytotoxic T lymphocytes (CTL) and ELISA antibodies. Immunization with a mixture of both gB and pp65 plasmids raised antibodies to both proteins and pp65-specific CTL, indicating a lack of interference between these two plasmids. These results suggest that DNA immunization is a useful approach for vaccination against HCMV disease.</description><identifier>ISSN: 0264-410X</identifier><identifier>EISSN: 1873-2518</identifier><identifier>DOI: 10.1016/S0264-410X(98)00145-5</identifier><identifier>PMID: 10078607</identifier><identifier>CODEN: VACCDE</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Animals ; Antibodies, Viral - biosynthesis ; antibody ; Antibody Specificity ; Biological and medical sciences ; CTL ; Cytomegalovirus - immunology ; Cytotoxicity, Immunologic ; DNA immunization ; Enzyme-Linked Immunosorbent Assay ; Female ; Fundamental and applied biological sciences. Psychology ; HCMV ; Human cytomegalovirus ; Humans ; Immunoglobulin G - biosynthesis ; Immunoglobulin Isotypes - biosynthesis ; Lymphocyte Activation ; Membranes - metabolism ; Mice ; Mice, Inbred BALB C ; Microbiology ; Neutralization Tests ; Phosphoproteins - genetics ; Phosphoproteins - immunology ; Plasmids - genetics ; T-Lymphocytes, Cytotoxic - immunology ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies ; Vaccines, DNA - immunology ; Vaccines, DNA - pharmacology ; Viral Envelope Proteins - genetics ; Viral Envelope Proteins - immunology ; Viral Matrix Proteins - genetics ; Viral Matrix Proteins - immunology ; Virology</subject><ispartof>Vaccine, 1999, Vol.17 (1), p.50-58</ispartof><rights>1998 Elsevier Science Ltd</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-2ea22ce80bc809bcd1bcc03c3b74e1a083c1a57d057466d50a329014cfbf18793</citedby><cites>FETCH-LOGICAL-c421t-2ea22ce80bc809bcd1bcc03c3b74e1a083c1a57d057466d50a329014cfbf18793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0264-410X(98)00145-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,4025,27928,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1664528$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10078607$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Endresz, Valeria</creatorcontrib><creatorcontrib>Kari, Laszlo</creatorcontrib><creatorcontrib>Berencsi, Klara</creatorcontrib><creatorcontrib>Kari, Csaba</creatorcontrib><creatorcontrib>Gyulai, Zsofia</creatorcontrib><creatorcontrib>Jeney, Csaba</creatorcontrib><creatorcontrib>Pincus, Steve</creatorcontrib><creatorcontrib>Rodeck, Ulrich</creatorcontrib><creatorcontrib>Méric, Claude</creatorcontrib><creatorcontrib>Plotkin, Stanley A</creatorcontrib><creatorcontrib>Gönczöl, Eva</creatorcontrib><title>Induction of human cytomegalovirus (HCMV)-glycoprotein B (gB)-specific neutralizing antibody and phosphoprotein 65 (pp65)-specific cytotoxic T lymphocyte responses by naked DNA immunization</title><title>Vaccine</title><addtitle>Vaccine</addtitle><description>Plasmids expressing the human cytomegalovirus (HCMV) glycoprotein B (gB) (UL55) or phosphoprotein 65 (pp65) (UL83) were constructed and evaluated for their ability to induce immune responses in mice. The full-length gB as well as a truncated form expressing amino acids 1–680 of gB, and lacking the fragment encoding amino acids 681–907 including the transmembrane domain of gB (gB680) were evaluated. Immunization of mice with plasmids coding for gB or gB680 induced ELISA and neutralizing antibodies, with the highest titres in mice immunized with the gB680 plasmid. Mice immunized with the gB plasmid predominantly produced IgG2a gB-specific antibody, while the gB680 plasmid raised mostly IgG1 anti-gB antibody. Mice immunized with the pp65 plasmid developed pp65-specific cytotoxic T lymphocytes (CTL) and ELISA antibodies. Immunization with a mixture of both gB and pp65 plasmids raised antibodies to both proteins and pp65-specific CTL, indicating a lack of interference between these two plasmids. These results suggest that DNA immunization is a useful approach for vaccination against HCMV disease.</description><subject>Animals</subject><subject>Antibodies, Viral - biosynthesis</subject><subject>antibody</subject><subject>Antibody Specificity</subject><subject>Biological and medical sciences</subject><subject>CTL</subject><subject>Cytomegalovirus - immunology</subject><subject>Cytotoxicity, Immunologic</subject><subject>DNA immunization</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>HCMV</topic><topic>Human cytomegalovirus</topic><topic>Humans</topic><topic>Immunoglobulin G - biosynthesis</topic><topic>Immunoglobulin Isotypes - biosynthesis</topic><topic>Lymphocyte Activation</topic><topic>Membranes - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>Neutralization Tests</topic><topic>Phosphoproteins - genetics</topic><topic>Phosphoproteins - immunology</topic><topic>Plasmids - genetics</topic><topic>T-Lymphocytes, Cytotoxic - immunology</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Vaccines, DNA - immunology</topic><topic>Vaccines, DNA - pharmacology</topic><topic>Viral Envelope Proteins - genetics</topic><topic>Viral Envelope Proteins - immunology</topic><topic>Viral Matrix Proteins - genetics</topic><topic>Viral Matrix Proteins - immunology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Endresz, Valeria</creatorcontrib><creatorcontrib>Kari, Laszlo</creatorcontrib><creatorcontrib>Berencsi, Klara</creatorcontrib><creatorcontrib>Kari, Csaba</creatorcontrib><creatorcontrib>Gyulai, Zsofia</creatorcontrib><creatorcontrib>Jeney, Csaba</creatorcontrib><creatorcontrib>Pincus, Steve</creatorcontrib><creatorcontrib>Rodeck, Ulrich</creatorcontrib><creatorcontrib>Méric, Claude</creatorcontrib><creatorcontrib>Plotkin, Stanley A</creatorcontrib><creatorcontrib>Gönczöl, Eva</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Vaccine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Endresz, Valeria</au><au>Kari, Laszlo</au><au>Berencsi, Klara</au><au>Kari, Csaba</au><au>Gyulai, Zsofia</au><au>Jeney, Csaba</au><au>Pincus, Steve</au><au>Rodeck, Ulrich</au><au>Méric, Claude</au><au>Plotkin, Stanley A</au><au>Gönczöl, Eva</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of human cytomegalovirus (HCMV)-glycoprotein B (gB)-specific neutralizing antibody and phosphoprotein 65 (pp65)-specific cytotoxic T lymphocyte responses by naked DNA immunization</atitle><jtitle>Vaccine</jtitle><addtitle>Vaccine</addtitle><date>1999</date><risdate>1999</risdate><volume>17</volume><issue>1</issue><spage>50</spage><epage>58</epage><pages>50-58</pages><issn>0264-410X</issn><eissn>1873-2518</eissn><coden>VACCDE</coden><abstract>Plasmids expressing the human cytomegalovirus (HCMV) glycoprotein B (gB) (UL55) or phosphoprotein 65 (pp65) (UL83) were constructed and evaluated for their ability to induce immune responses in mice. The full-length gB as well as a truncated form expressing amino acids 1–680 of gB, and lacking the fragment encoding amino acids 681–907 including the transmembrane domain of gB (gB680) were evaluated. Immunization of mice with plasmids coding for gB or gB680 induced ELISA and neutralizing antibodies, with the highest titres in mice immunized with the gB680 plasmid. Mice immunized with the gB plasmid predominantly produced IgG2a gB-specific antibody, while the gB680 plasmid raised mostly IgG1 anti-gB antibody. Mice immunized with the pp65 plasmid developed pp65-specific cytotoxic T lymphocytes (CTL) and ELISA antibodies. Immunization with a mixture of both gB and pp65 plasmids raised antibodies to both proteins and pp65-specific CTL, indicating a lack of interference between these two plasmids. These results suggest that DNA immunization is a useful approach for vaccination against HCMV disease.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>10078607</pmid><doi>10.1016/S0264-410X(98)00145-5</doi><tpages>9</tpages></addata></record> |
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| ispartof | Vaccine, 1999, Vol.17 (1), p.50-58 |
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| subjects | Animals Antibodies, Viral - biosynthesis antibody Antibody Specificity Biological and medical sciences CTL Cytomegalovirus - immunology Cytotoxicity, Immunologic DNA immunization Enzyme-Linked Immunosorbent Assay Female Fundamental and applied biological sciences. Psychology HCMV Human cytomegalovirus Humans Immunoglobulin G - biosynthesis Immunoglobulin Isotypes - biosynthesis Lymphocyte Activation Membranes - metabolism Mice Mice, Inbred BALB C Microbiology Neutralization Tests Phosphoproteins - genetics Phosphoproteins - immunology Plasmids - genetics T-Lymphocytes, Cytotoxic - immunology Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vaccines, DNA - immunology Vaccines, DNA - pharmacology Viral Envelope Proteins - genetics Viral Envelope Proteins - immunology Viral Matrix Proteins - genetics Viral Matrix Proteins - immunology Virology |
| title | Induction of human cytomegalovirus (HCMV)-glycoprotein B (gB)-specific neutralizing antibody and phosphoprotein 65 (pp65)-specific cytotoxic T lymphocyte responses by naked DNA immunization |
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