Evidence for the involvement of platelet-derived growth factor in the angiotensin II-induced growth of rat vascular smooth muscle cells
This study examines the possible involvement of endogenous platelet-derived growth factor (PDGF) in the angiotensin II-induced growth of rat aortic smooth muscle cells. In quiescent confluent cells, anti-PDGF-AB neutralizing antibody inhibited angiotensin II-induced DNA synthesis and protein synthes...
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| Veröffentlicht in: | Biological & pharmaceutical bulletin 1999, Vol.22 (2), p.137-141 |
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| creator | HANADA, M SAITO, E KAMBE, T HAGIWARA, Y KUBO, T |
| description | This study examines the possible involvement of endogenous platelet-derived growth factor (PDGF) in the angiotensin II-induced growth of rat aortic smooth muscle cells. In quiescent confluent cells, anti-PDGF-AB neutralizing antibody inhibited angiotensin II-induced DNA synthesis and protein synthesis. PDGF-AA, -AB, and -BB produced concentration-dependent increases in DNA synthesis and protein synthesis. Genistein did not inhibit PDGF-AB-induced [3H]thymidine incorporation and [3H]leucine incorporation. PDGF-AB stimulated mitogen-activated protein (MAP) kinases, and PDGF-induced MAP kinase activation was inhibited by genistein. Angiotensin II induced PDGF-A chain messenger RNA expression, and genistein inhibited angiotensin-induced PDGF gene expression. These findings suggest that endogenous PDGF is, at least in part, involved in angiotensin II-induced cell growth in rat vascular smooth muscle cells. It appears that genistein inhibits angiotensin II-induced DNA synthesis partly by inhibiting PDGF-A gene expression. |
| doi_str_mv | 10.1248/bpb.22.137 |
| format | Article |
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In quiescent confluent cells, anti-PDGF-AB neutralizing antibody inhibited angiotensin II-induced DNA synthesis and protein synthesis. PDGF-AA, -AB, and -BB produced concentration-dependent increases in DNA synthesis and protein synthesis. Genistein did not inhibit PDGF-AB-induced [3H]thymidine incorporation and [3H]leucine incorporation. PDGF-AB stimulated mitogen-activated protein (MAP) kinases, and PDGF-induced MAP kinase activation was inhibited by genistein. Angiotensin II induced PDGF-A chain messenger RNA expression, and genistein inhibited angiotensin-induced PDGF gene expression. These findings suggest that endogenous PDGF is, at least in part, involved in angiotensin II-induced cell growth in rat vascular smooth muscle cells. It appears that genistein inhibits angiotensin II-induced DNA synthesis partly by inhibiting PDGF-A gene expression.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.22.137</identifier><identifier>PMID: 10077431</identifier><language>eng</language><publisher>Tokyo: Maruzen</publisher><subject>Angiotensin II - pharmacology ; Animals ; Antibodies - immunology ; Biological and medical sciences ; Blood vessels and receptors ; Calcium-Calmodulin-Dependent Protein Kinases - metabolism ; Cell Division - drug effects ; Cell Division - physiology ; Cells, Cultured ; DNA Replication - drug effects ; DNA Replication - physiology ; Enzyme Activation ; Fundamental and applied biological sciences. Psychology ; Genistein - pharmacology ; Male ; Muscle Proteins - biosynthesis ; Muscle, Smooth, Vascular - drug effects ; Muscle, Smooth, Vascular - enzymology ; Muscle, Smooth, Vascular - metabolism ; Neutralization Tests ; Platelet-Derived Growth Factor - genetics ; Platelet-Derived Growth Factor - immunology ; Platelet-Derived Growth Factor - physiology ; Rats ; Rats, Wistar ; RNA, Messenger - genetics ; Vertebrates: cardiovascular system</subject><ispartof>Biological & pharmaceutical bulletin, 1999, Vol.22 (2), p.137-141</ispartof><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c540t-13e2474a6545620629003ddca5df63c4e66901c224fd477f8b111680876a91043</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1733695$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10077431$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HANADA, M</creatorcontrib><creatorcontrib>SAITO, E</creatorcontrib><creatorcontrib>KAMBE, T</creatorcontrib><creatorcontrib>HAGIWARA, Y</creatorcontrib><creatorcontrib>KUBO, T</creatorcontrib><title>Evidence for the involvement of platelet-derived growth factor in the angiotensin II-induced growth of rat vascular smooth muscle cells</title><title>Biological & pharmaceutical bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>This study examines the possible involvement of endogenous platelet-derived growth factor (PDGF) in the angiotensin II-induced growth of rat aortic smooth muscle cells. In quiescent confluent cells, anti-PDGF-AB neutralizing antibody inhibited angiotensin II-induced DNA synthesis and protein synthesis. PDGF-AA, -AB, and -BB produced concentration-dependent increases in DNA synthesis and protein synthesis. Genistein did not inhibit PDGF-AB-induced [3H]thymidine incorporation and [3H]leucine incorporation. PDGF-AB stimulated mitogen-activated protein (MAP) kinases, and PDGF-induced MAP kinase activation was inhibited by genistein. Angiotensin II induced PDGF-A chain messenger RNA expression, and genistein inhibited angiotensin-induced PDGF gene expression. These findings suggest that endogenous PDGF is, at least in part, involved in angiotensin II-induced cell growth in rat vascular smooth muscle cells. It appears that genistein inhibits angiotensin II-induced DNA synthesis partly by inhibiting PDGF-A gene expression.</description><subject>Angiotensin II - pharmacology</subject><subject>Animals</subject><subject>Antibodies - immunology</subject><subject>Biological and medical sciences</subject><subject>Blood vessels and receptors</subject><subject>Calcium-Calmodulin-Dependent Protein Kinases - metabolism</subject><subject>Cell Division - drug effects</subject><subject>Cell Division - physiology</subject><subject>Cells, Cultured</subject><subject>DNA Replication - drug effects</subject><subject>DNA Replication - physiology</subject><subject>Enzyme Activation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genistein - pharmacology</subject><subject>Male</subject><subject>Muscle Proteins - biosynthesis</subject><subject>Muscle, Smooth, Vascular - drug effects</subject><subject>Muscle, Smooth, Vascular - enzymology</subject><subject>Muscle, Smooth, Vascular - metabolism</subject><subject>Neutralization Tests</subject><subject>Platelet-Derived Growth Factor - genetics</subject><subject>Platelet-Derived Growth Factor - immunology</subject><subject>Platelet-Derived Growth Factor - physiology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>RNA, Messenger - genetics</subject><subject>Vertebrates: cardiovascular system</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkE1rFTEUhoNY7G114w-QLKQLYa75npmllFYvFNzU9ZCbnLSRTHJNMlP8Bf5tU--FujofPOfl8CD0npItZWL4vD_st4xtKe9foQ3lou8ko_I12pCRDp2icjhHF6X8JIT0hPE36Jy2rhecbtCfm9VbiAawSxnXR8A-rimsMEOsODl8CLpCgNpZyH4Fix9yeqqP2GlT24WP_450fPCpQixt3u06H-1iXtgWk3XFqy5mCTrjMqfU1vNSTABsIITyFp05HQq8O9VL9OP25v76W3f3_evu-stdZ6QgtaMcmOiFVlJIxYhiIyHcWqOldYobAUqNhBrGhLOi792wp5SqgQy90iMlgl-iq2PuIadfC5Q6zb48f6AjpKVMalSMSSYb-OkImpxKyeCmQ_azzr8nSqZn7VPTPjE2Ne0N_nBKXfYz2P_Qo-cGfDwBzYEOLutofHnhes7VKPlf96uLRA</recordid><startdate>1999</startdate><enddate>1999</enddate><creator>HANADA, M</creator><creator>SAITO, E</creator><creator>KAMBE, T</creator><creator>HAGIWARA, Y</creator><creator>KUBO, T</creator><general>Maruzen</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1999</creationdate><title>Evidence for the involvement of platelet-derived growth factor in the angiotensin II-induced growth of rat vascular smooth muscle cells</title><author>HANADA, M ; SAITO, E ; KAMBE, T ; HAGIWARA, Y ; KUBO, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c540t-13e2474a6545620629003ddca5df63c4e66901c224fd477f8b111680876a91043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Angiotensin II - pharmacology</topic><topic>Animals</topic><topic>Antibodies - immunology</topic><topic>Biological and medical sciences</topic><topic>Blood vessels and receptors</topic><topic>Calcium-Calmodulin-Dependent Protein Kinases - metabolism</topic><topic>Cell Division - drug effects</topic><topic>Cell Division - physiology</topic><topic>Cells, Cultured</topic><topic>DNA Replication - drug effects</topic><topic>DNA Replication - physiology</topic><topic>Enzyme Activation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genistein - pharmacology</topic><topic>Male</topic><topic>Muscle Proteins - biosynthesis</topic><topic>Muscle, Smooth, Vascular - drug effects</topic><topic>Muscle, Smooth, Vascular - enzymology</topic><topic>Muscle, Smooth, Vascular - metabolism</topic><topic>Neutralization Tests</topic><topic>Platelet-Derived Growth Factor - genetics</topic><topic>Platelet-Derived Growth Factor - immunology</topic><topic>Platelet-Derived Growth Factor - physiology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>RNA, Messenger - genetics</topic><topic>Vertebrates: cardiovascular system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HANADA, M</creatorcontrib><creatorcontrib>SAITO, E</creatorcontrib><creatorcontrib>KAMBE, T</creatorcontrib><creatorcontrib>HAGIWARA, Y</creatorcontrib><creatorcontrib>KUBO, T</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biological & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HANADA, M</au><au>SAITO, E</au><au>KAMBE, T</au><au>HAGIWARA, Y</au><au>KUBO, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evidence for the involvement of platelet-derived growth factor in the angiotensin II-induced growth of rat vascular smooth muscle cells</atitle><jtitle>Biological & pharmaceutical bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>1999</date><risdate>1999</risdate><volume>22</volume><issue>2</issue><spage>137</spage><epage>141</epage><pages>137-141</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>This study examines the possible involvement of endogenous platelet-derived growth factor (PDGF) in the angiotensin II-induced growth of rat aortic smooth muscle cells. In quiescent confluent cells, anti-PDGF-AB neutralizing antibody inhibited angiotensin II-induced DNA synthesis and protein synthesis. PDGF-AA, -AB, and -BB produced concentration-dependent increases in DNA synthesis and protein synthesis. Genistein did not inhibit PDGF-AB-induced [3H]thymidine incorporation and [3H]leucine incorporation. PDGF-AB stimulated mitogen-activated protein (MAP) kinases, and PDGF-induced MAP kinase activation was inhibited by genistein. Angiotensin II induced PDGF-A chain messenger RNA expression, and genistein inhibited angiotensin-induced PDGF gene expression. These findings suggest that endogenous PDGF is, at least in part, involved in angiotensin II-induced cell growth in rat vascular smooth muscle cells. It appears that genistein inhibits angiotensin II-induced DNA synthesis partly by inhibiting PDGF-A gene expression.</abstract><cop>Tokyo</cop><pub>Maruzen</pub><pmid>10077431</pmid><doi>10.1248/bpb.22.137</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | J-STAGE Free; MEDLINE; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Angiotensin II - pharmacology Animals Antibodies - immunology Biological and medical sciences Blood vessels and receptors Calcium-Calmodulin-Dependent Protein Kinases - metabolism Cell Division - drug effects Cell Division - physiology Cells, Cultured DNA Replication - drug effects DNA Replication - physiology Enzyme Activation Fundamental and applied biological sciences. Psychology Genistein - pharmacology Male Muscle Proteins - biosynthesis Muscle, Smooth, Vascular - drug effects Muscle, Smooth, Vascular - enzymology Muscle, Smooth, Vascular - metabolism Neutralization Tests Platelet-Derived Growth Factor - genetics Platelet-Derived Growth Factor - immunology Platelet-Derived Growth Factor - physiology Rats Rats, Wistar RNA, Messenger - genetics Vertebrates: cardiovascular system |
| title | Evidence for the involvement of platelet-derived growth factor in the angiotensin II-induced growth of rat vascular smooth muscle cells |
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