Cementum and Periodontal Ligament–like Tissue Formation Induced Using Bioengineered Dentin
Stem cell–mediated root regeneration offers opportunities to regenerate a bio-root and its associated periodontal tissues to restore tooth loss. Periodontal ligament (PDL) and cementum complex and dentin pulp complex have been tissue engineered using human dental pulp stem cells and PDL stem cells,...
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| Veröffentlicht in: | Tissue engineering. Part A 2008-10, Vol.14 (10), p.1731-1742 |
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| container_title | Tissue engineering. Part A |
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| creator | Li, Yucheng Jin, Fang Du, Yan Ma, Zhiwei Li, Fang Wu, Gang Shi, Junnan Zhu, Xiaoru Yu, Jinhua Jin, Yan |
| description | Stem cell–mediated root regeneration offers opportunities to regenerate a bio-root and its associated periodontal tissues to restore tooth loss. Periodontal ligament (PDL) and cementum complex and dentin pulp complex have been tissue engineered using human dental pulp stem cells and PDL stem cells, respectively. The aim of this study was to explore whether dentin formation could be induced using an inductive substrate and whether bioengineered dentin could induce cementum and PDL formation. First, dentin was bioengineered from tooth papillae of Sprague-Dawley (SD) rats with an inductive substrate, and its phenotype was characterized; then primarily cultured human PDL cells were seeded on the surface of dentin and transplanted under the skin of immunocompromised mice. Histological, immunohistochemical, and scanning electronic microscopy examinations results showed that bioengineered dentin could induce cementogenesis and PDL formation, and condense PDL arranged perpendicularly on the dentin surface via a layer of cementum-like tissue. The results indicated that tissue-engineered dentin could be induced using an inductive substrate and could be used as a further substrate for cementum and PDL tissue engineering. |
| doi_str_mv | 10.1089/ten.tea.2007.0268 |
| format | Article |
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Periodontal ligament (PDL) and cementum complex and dentin pulp complex have been tissue engineered using human dental pulp stem cells and PDL stem cells, respectively. The aim of this study was to explore whether dentin formation could be induced using an inductive substrate and whether bioengineered dentin could induce cementum and PDL formation. First, dentin was bioengineered from tooth papillae of Sprague-Dawley (SD) rats with an inductive substrate, and its phenotype was characterized; then primarily cultured human PDL cells were seeded on the surface of dentin and transplanted under the skin of immunocompromised mice. Histological, immunohistochemical, and scanning electronic microscopy examinations results showed that bioengineered dentin could induce cementogenesis and PDL formation, and condense PDL arranged perpendicularly on the dentin surface via a layer of cementum-like tissue. The results indicated that tissue-engineered dentin could be induced using an inductive substrate and could be used as a further substrate for cementum and PDL tissue engineering.</description><identifier>ISSN: 1937-3341</identifier><identifier>EISSN: 1937-335X</identifier><identifier>DOI: 10.1089/ten.tea.2007.0268</identifier><identifier>PMID: 18636796</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Animals ; Bioengineering ; Cells, Cultured ; Collagen - metabolism ; Dental Cementum - cytology ; Dental Cementum - metabolism ; Dental Cementum - ultrastructure ; Dentin ; Dentin - transplantation ; Guided Tissue Regeneration, Periodontal - methods ; Humans ; Immunohistochemistry ; Methods ; Mice ; Microscopy, Electron, Scanning ; Periodontal Ligament - cytology ; Periodontal Ligament - metabolism ; Periodontal Ligament - ultrastructure ; Physiological aspects ; Prevention ; Rats ; Rats, Sprague-Dawley ; Stem cells ; Tissue engineering ; Tissue Engineering - methods ; Tooth loss</subject><ispartof>Tissue engineering. Part A, 2008-10, Vol.14 (10), p.1731-1742</ispartof><rights>2008, Mary Ann Liebert, Inc.</rights><rights>COPYRIGHT 2008 Mary Ann Liebert, Inc.</rights><rights>(©) Copyright 2008, Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c472t-64f85b7f2b5bb66114c419e3093868b7841ee7a970f7c6a4b0d8cdd3fdbf835e3</citedby><cites>FETCH-LOGICAL-c472t-64f85b7f2b5bb66114c419e3093868b7841ee7a970f7c6a4b0d8cdd3fdbf835e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18636796$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Yucheng</creatorcontrib><creatorcontrib>Jin, Fang</creatorcontrib><creatorcontrib>Du, Yan</creatorcontrib><creatorcontrib>Ma, Zhiwei</creatorcontrib><creatorcontrib>Li, Fang</creatorcontrib><creatorcontrib>Wu, Gang</creatorcontrib><creatorcontrib>Shi, Junnan</creatorcontrib><creatorcontrib>Zhu, Xiaoru</creatorcontrib><creatorcontrib>Yu, Jinhua</creatorcontrib><creatorcontrib>Jin, Yan</creatorcontrib><title>Cementum and Periodontal Ligament–like Tissue Formation Induced Using Bioengineered Dentin</title><title>Tissue engineering. Part A</title><addtitle>Tissue Eng Part A</addtitle><description>Stem cell–mediated root regeneration offers opportunities to regenerate a bio-root and its associated periodontal tissues to restore tooth loss. Periodontal ligament (PDL) and cementum complex and dentin pulp complex have been tissue engineered using human dental pulp stem cells and PDL stem cells, respectively. The aim of this study was to explore whether dentin formation could be induced using an inductive substrate and whether bioengineered dentin could induce cementum and PDL formation. First, dentin was bioengineered from tooth papillae of Sprague-Dawley (SD) rats with an inductive substrate, and its phenotype was characterized; then primarily cultured human PDL cells were seeded on the surface of dentin and transplanted under the skin of immunocompromised mice. Histological, immunohistochemical, and scanning electronic microscopy examinations results showed that bioengineered dentin could induce cementogenesis and PDL formation, and condense PDL arranged perpendicularly on the dentin surface via a layer of cementum-like tissue. The results indicated that tissue-engineered dentin could be induced using an inductive substrate and could be used as a further substrate for cementum and PDL tissue engineering.</description><subject>Animals</subject><subject>Bioengineering</subject><subject>Cells, Cultured</subject><subject>Collagen - metabolism</subject><subject>Dental Cementum - cytology</subject><subject>Dental Cementum - metabolism</subject><subject>Dental Cementum - ultrastructure</subject><subject>Dentin</subject><subject>Dentin - transplantation</subject><subject>Guided Tissue Regeneration, Periodontal - methods</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Methods</subject><subject>Mice</subject><subject>Microscopy, Electron, Scanning</subject><subject>Periodontal Ligament - cytology</subject><subject>Periodontal Ligament - metabolism</subject><subject>Periodontal Ligament - ultrastructure</subject><subject>Physiological aspects</subject><subject>Prevention</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Stem cells</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - methods</subject><subject>Tooth loss</subject><issn>1937-3341</issn><issn>1937-335X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkc2KFDEUhYMozjj6AG6kUHDXZX6q8rMcW0cHGnQxAy6EkKrcNBmrkjFJLdz5Dr6hT2KKbhRFUELI5eQ7h4SD0GOCW4KlelEgtAVMSzEWLaZc3kGnRDGxYaz_cPfn3JET9CDnG4w55kLcRydEcsaF4qfo4xZmCGWZGxNs8x6SjzaGYqZm5_dmvfr-9dvkP0Fz5XNeoLmIaTbFx9BcBruMYJvr7MO-eekjhL0PAKlqr6rRh4fonjNThkfH8wxdX7y-2r7d7N69udye7zZjJ2jZ8M7JfhCODv0wcE5IN3ZEAcOKSS4HITsCIIwS2ImRm27AVo7WMmcHJ1kP7Aw9P-Tepvh5gVz07PMI02QCxCVrrjilTPX_BImisldcVvDpH-BNXFKon9AUE8E4laRCzw7Q3kygfXCxJDOuifqcSNFzRuUa1f6FqsvC7McYwPmq_2YgB8OYYs4JnL5NfjbpiyZYr73r2nvdRq-967X36nlyfO8yzGB_OY5FV0AcgFU2IUweBkjlP6J_ABo-vO0</recordid><startdate>20081001</startdate><enddate>20081001</enddate><creator>Li, Yucheng</creator><creator>Jin, Fang</creator><creator>Du, Yan</creator><creator>Ma, Zhiwei</creator><creator>Li, Fang</creator><creator>Wu, Gang</creator><creator>Shi, Junnan</creator><creator>Zhu, Xiaoru</creator><creator>Yu, Jinhua</creator><creator>Jin, Yan</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20081001</creationdate><title>Cementum and Periodontal Ligament–like Tissue Formation Induced Using Bioengineered Dentin</title><author>Li, Yucheng ; 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Part A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Yucheng</au><au>Jin, Fang</au><au>Du, Yan</au><au>Ma, Zhiwei</au><au>Li, Fang</au><au>Wu, Gang</au><au>Shi, Junnan</au><au>Zhu, Xiaoru</au><au>Yu, Jinhua</au><au>Jin, Yan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cementum and Periodontal Ligament–like Tissue Formation Induced Using Bioengineered Dentin</atitle><jtitle>Tissue engineering. Part A</jtitle><addtitle>Tissue Eng Part A</addtitle><date>2008-10-01</date><risdate>2008</risdate><volume>14</volume><issue>10</issue><spage>1731</spage><epage>1742</epage><pages>1731-1742</pages><issn>1937-3341</issn><eissn>1937-335X</eissn><abstract>Stem cell–mediated root regeneration offers opportunities to regenerate a bio-root and its associated periodontal tissues to restore tooth loss. Periodontal ligament (PDL) and cementum complex and dentin pulp complex have been tissue engineered using human dental pulp stem cells and PDL stem cells, respectively. The aim of this study was to explore whether dentin formation could be induced using an inductive substrate and whether bioengineered dentin could induce cementum and PDL formation. First, dentin was bioengineered from tooth papillae of Sprague-Dawley (SD) rats with an inductive substrate, and its phenotype was characterized; then primarily cultured human PDL cells were seeded on the surface of dentin and transplanted under the skin of immunocompromised mice. Histological, immunohistochemical, and scanning electronic microscopy examinations results showed that bioengineered dentin could induce cementogenesis and PDL formation, and condense PDL arranged perpendicularly on the dentin surface via a layer of cementum-like tissue. The results indicated that tissue-engineered dentin could be induced using an inductive substrate and could be used as a further substrate for cementum and PDL tissue engineering.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>18636796</pmid><doi>10.1089/ten.tea.2007.0268</doi><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1937-3341 |
| ispartof | Tissue engineering. Part A, 2008-10, Vol.14 (10), p.1731-1742 |
| issn | 1937-3341 1937-335X |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Bioengineering Cells, Cultured Collagen - metabolism Dental Cementum - cytology Dental Cementum - metabolism Dental Cementum - ultrastructure Dentin Dentin - transplantation Guided Tissue Regeneration, Periodontal - methods Humans Immunohistochemistry Methods Mice Microscopy, Electron, Scanning Periodontal Ligament - cytology Periodontal Ligament - metabolism Periodontal Ligament - ultrastructure Physiological aspects Prevention Rats Rats, Sprague-Dawley Stem cells Tissue engineering Tissue Engineering - methods Tooth loss |
| title | Cementum and Periodontal Ligament–like Tissue Formation Induced Using Bioengineered Dentin |
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