GPR56, a Novel Secretin-like Human G-Protein-Coupled Receptor Gene
A novel gene product, GPR56, with homology to the seven transmembrane-domain receptor superfamily, has been cloned by PCR amplification using degenerate oligonucleotide primers and subsequent screening of a human heart cDNA library. The isolated 2.8-kb cDNA clone encodes a protein of 693 amino acids...
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| Veröffentlicht in: | Genomics (San Diego, Calif.) Calif.), 1999-02, Vol.55 (3), p.296-305 |
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| container_title | Genomics (San Diego, Calif.) |
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| creator | Liu, Marjorie Parker, Rachel M.C. Darby, Karen Eyre, Helen J. Copeland, Neal G. Crawford, Joanna Gilbert, Debra J. Sutherland, Grant R. Jenkins, Nancy A. Herzog, Herbert |
| description | A novel gene product, GPR56, with homology to the seven transmembrane-domain receptor superfamily, has been cloned by PCR amplification using degenerate oligonucleotide primers and subsequent screening of a human heart cDNA library. The isolated 2.8-kb cDNA clone encodes a protein of 693 amino acids that shows highest identity (32%) to HE6, a member of a subclass of the class B secretin-like G-protein-coupled receptors. Northern analysis of various human tissues revealed a wide distribution of the transcript with highest levels found in thyroid gland, brain, and heart.In situhybridization analysis of human thyroid gland as well as rat heart and brain tissue confirms these results and identifies the hippocampus and hypothalamic nuclei as brain areas with particularly high expression of GPR56 mRNA. The high level of mRNA expression, its wide distribution, and the mucin-like extracellular domain of the receptor protein suggest a possible role for this receptor in cell–cell interaction processes. The human gene for GPR56 has been isolated and its exon–intron structure determined. The total length of the human GPR56 gene is approximately 15 kb, and it consists of 13 exons. Fluorescencein situhybridization, PCR analysis of somatic cell hybrids, and interspecific mouse backcross mapping have localized the genes to human chromosome 16q13 and mouse chromosome 8. |
| doi_str_mv | 10.1006/geno.1998.5644 |
| format | Article |
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The isolated 2.8-kb cDNA clone encodes a protein of 693 amino acids that shows highest identity (32%) to HE6, a member of a subclass of the class B secretin-like G-protein-coupled receptors. Northern analysis of various human tissues revealed a wide distribution of the transcript with highest levels found in thyroid gland, brain, and heart.In situhybridization analysis of human thyroid gland as well as rat heart and brain tissue confirms these results and identifies the hippocampus and hypothalamic nuclei as brain areas with particularly high expression of GPR56 mRNA. The high level of mRNA expression, its wide distribution, and the mucin-like extracellular domain of the receptor protein suggest a possible role for this receptor in cell–cell interaction processes. The human gene for GPR56 has been isolated and its exon–intron structure determined. The total length of the human GPR56 gene is approximately 15 kb, and it consists of 13 exons. Fluorescencein situhybridization, PCR analysis of somatic cell hybrids, and interspecific mouse backcross mapping have localized the genes to human chromosome 16q13 and mouse chromosome 8.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1006/geno.1998.5644</identifier><identifier>PMID: 10049584</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; Blotting, Northern ; Chromosome Mapping ; Chromosomes, Human, Pair 16 - genetics ; Crosses, Genetic ; DNA Primers ; Exons ; Fundamental and applied biological sciences. Psychology ; Gene Library ; Genes. Genome ; GTP-Binding Proteins - genetics ; Humans ; Hypothalamus - cytology ; Hypothalamus - ultrastructure ; In Situ Hybridization ; In Situ Hybridization, Fluorescence ; Introns ; Metaphase ; Mice ; Mice, Inbred C57BL ; Models, Biological ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Myocardium - cytology ; Myocardium - metabolism ; Myocardium - ultrastructure ; Rats ; Receptors, Cell Surface - genetics ; Receptors, G-Protein-Coupled ; Secretin - genetics ; Thyroid Gland - cytology ; Thyroid Gland - metabolism ; Tissue Distribution</subject><ispartof>Genomics (San Diego, Calif.), 1999-02, Vol.55 (3), p.296-305</ispartof><rights>1999 Academic Press</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-ce8f8b9f34deb52e12dfc2d021524bed25a05a743b9c832b26f61d993c54eb693</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/geno.1998.5644$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1710062$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10049584$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liu, Marjorie</creatorcontrib><creatorcontrib>Parker, Rachel M.C.</creatorcontrib><creatorcontrib>Darby, Karen</creatorcontrib><creatorcontrib>Eyre, Helen J.</creatorcontrib><creatorcontrib>Copeland, Neal G.</creatorcontrib><creatorcontrib>Crawford, Joanna</creatorcontrib><creatorcontrib>Gilbert, Debra J.</creatorcontrib><creatorcontrib>Sutherland, Grant R.</creatorcontrib><creatorcontrib>Jenkins, Nancy A.</creatorcontrib><creatorcontrib>Herzog, Herbert</creatorcontrib><title>GPR56, a Novel Secretin-like Human G-Protein-Coupled Receptor Gene</title><title>Genomics (San Diego, Calif.)</title><addtitle>Genomics</addtitle><description>A novel gene product, GPR56, with homology to the seven transmembrane-domain receptor superfamily, has been cloned by PCR amplification using degenerate oligonucleotide primers and subsequent screening of a human heart cDNA library. The isolated 2.8-kb cDNA clone encodes a protein of 693 amino acids that shows highest identity (32%) to HE6, a member of a subclass of the class B secretin-like G-protein-coupled receptors. Northern analysis of various human tissues revealed a wide distribution of the transcript with highest levels found in thyroid gland, brain, and heart.In situhybridization analysis of human thyroid gland as well as rat heart and brain tissue confirms these results and identifies the hippocampus and hypothalamic nuclei as brain areas with particularly high expression of GPR56 mRNA. The high level of mRNA expression, its wide distribution, and the mucin-like extracellular domain of the receptor protein suggest a possible role for this receptor in cell–cell interaction processes. The human gene for GPR56 has been isolated and its exon–intron structure determined. The total length of the human GPR56 gene is approximately 15 kb, and it consists of 13 exons. Fluorescencein situhybridization, PCR analysis of somatic cell hybrids, and interspecific mouse backcross mapping have localized the genes to human chromosome 16q13 and mouse chromosome 8.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Human, Pair 16 - genetics</subject><subject>Crosses, Genetic</subject><subject>DNA Primers</subject><subject>Exons</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Library</subject><subject>Genes. Genome</subject><subject>GTP-Binding Proteins - genetics</subject><subject>Humans</subject><subject>Hypothalamus - cytology</subject><subject>Hypothalamus - ultrastructure</subject><subject>In Situ Hybridization</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Introns</subject><subject>Metaphase</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Models, Biological</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Myocardium - cytology</subject><subject>Myocardium - metabolism</subject><subject>Myocardium - ultrastructure</subject><subject>Rats</subject><subject>Receptors, Cell Surface - genetics</subject><subject>Receptors, G-Protein-Coupled</subject><subject>Secretin - genetics</subject><subject>Thyroid Gland - cytology</subject><subject>Thyroid Gland - metabolism</subject><subject>Tissue Distribution</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLxDAUhYMoOj62LqULcWXHJE3SZKmDjoKo-FiHNLmVaKcZk1bw39syA7oRVxcu3zkcPoQOCZ4SjMXZK7RhSpSSUy4Y20ATgqXKpWBiE02wlDIvOSt20G5KbxhjVUi6jXaGKFNcsgm6mD88cnGamewufEKTPYGN0Pk2b_w7ZNf9wrTZPH-IoYPhOQv9sgGXPYKFZRdiNocW9tFWbZoEB-u7h16uLp9n1_nt_fxmdn6bW4Zxl1uQtaxUXTAHFadAqKstdZgSTlkFjnKDuSlZUSkrC1pRUQvilCosZ1AJVeyhk1XvMoaPHlKnFz5ZaBrTQuiTFkqQgiv6L0hKUg5WxsbpCrQxpBSh1svoFyZ-aYL1qFePevWoV496h8DRurmvFuB-4SufA3C8Bkyypqmjaa1PP1w5to4L5QqDwdenh6iT9dBacD6C7bQL_q8J31D6k_I</recordid><startdate>19990201</startdate><enddate>19990201</enddate><creator>Liu, Marjorie</creator><creator>Parker, Rachel M.C.</creator><creator>Darby, Karen</creator><creator>Eyre, Helen J.</creator><creator>Copeland, Neal G.</creator><creator>Crawford, Joanna</creator><creator>Gilbert, Debra J.</creator><creator>Sutherland, Grant R.</creator><creator>Jenkins, Nancy A.</creator><creator>Herzog, Herbert</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19990201</creationdate><title>GPR56, a Novel Secretin-like Human G-Protein-Coupled Receptor Gene</title><author>Liu, Marjorie ; Parker, Rachel M.C. ; Darby, Karen ; Eyre, Helen J. ; Copeland, Neal G. ; Crawford, Joanna ; Gilbert, Debra J. ; Sutherland, Grant R. ; Jenkins, Nancy A. ; Herzog, Herbert</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-ce8f8b9f34deb52e12dfc2d021524bed25a05a743b9c832b26f61d993c54eb693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Human, Pair 16 - genetics</topic><topic>Crosses, Genetic</topic><topic>DNA Primers</topic><topic>Exons</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Library</topic><topic>Genes. Genome</topic><topic>GTP-Binding Proteins - genetics</topic><topic>Humans</topic><topic>Hypothalamus - cytology</topic><topic>Hypothalamus - ultrastructure</topic><topic>In Situ Hybridization</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Introns</topic><topic>Metaphase</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Models, Biological</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Myocardium - cytology</topic><topic>Myocardium - metabolism</topic><topic>Myocardium - ultrastructure</topic><topic>Rats</topic><topic>Receptors, Cell Surface - genetics</topic><topic>Receptors, G-Protein-Coupled</topic><topic>Secretin - genetics</topic><topic>Thyroid Gland - cytology</topic><topic>Thyroid Gland - metabolism</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liu, Marjorie</creatorcontrib><creatorcontrib>Parker, Rachel M.C.</creatorcontrib><creatorcontrib>Darby, Karen</creatorcontrib><creatorcontrib>Eyre, Helen J.</creatorcontrib><creatorcontrib>Copeland, Neal G.</creatorcontrib><creatorcontrib>Crawford, Joanna</creatorcontrib><creatorcontrib>Gilbert, Debra J.</creatorcontrib><creatorcontrib>Sutherland, Grant R.</creatorcontrib><creatorcontrib>Jenkins, Nancy A.</creatorcontrib><creatorcontrib>Herzog, Herbert</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genomics (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liu, Marjorie</au><au>Parker, Rachel M.C.</au><au>Darby, Karen</au><au>Eyre, Helen J.</au><au>Copeland, Neal G.</au><au>Crawford, Joanna</au><au>Gilbert, Debra J.</au><au>Sutherland, Grant R.</au><au>Jenkins, Nancy A.</au><au>Herzog, Herbert</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>GPR56, a Novel Secretin-like Human G-Protein-Coupled Receptor Gene</atitle><jtitle>Genomics (San Diego, Calif.)</jtitle><addtitle>Genomics</addtitle><date>1999-02-01</date><risdate>1999</risdate><volume>55</volume><issue>3</issue><spage>296</spage><epage>305</epage><pages>296-305</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>A novel gene product, GPR56, with homology to the seven transmembrane-domain receptor superfamily, has been cloned by PCR amplification using degenerate oligonucleotide primers and subsequent screening of a human heart cDNA library. The isolated 2.8-kb cDNA clone encodes a protein of 693 amino acids that shows highest identity (32%) to HE6, a member of a subclass of the class B secretin-like G-protein-coupled receptors. Northern analysis of various human tissues revealed a wide distribution of the transcript with highest levels found in thyroid gland, brain, and heart.In situhybridization analysis of human thyroid gland as well as rat heart and brain tissue confirms these results and identifies the hippocampus and hypothalamic nuclei as brain areas with particularly high expression of GPR56 mRNA. The high level of mRNA expression, its wide distribution, and the mucin-like extracellular domain of the receptor protein suggest a possible role for this receptor in cell–cell interaction processes. The human gene for GPR56 has been isolated and its exon–intron structure determined. The total length of the human GPR56 gene is approximately 15 kb, and it consists of 13 exons. Fluorescencein situhybridization, PCR analysis of somatic cell hybrids, and interspecific mouse backcross mapping have localized the genes to human chromosome 16q13 and mouse chromosome 8.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>10049584</pmid><doi>10.1006/geno.1998.5644</doi><tpages>10</tpages></addata></record> |
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| ispartof | Genomics (San Diego, Calif.), 1999-02, Vol.55 (3), p.296-305 |
| issn | 0888-7543 1089-8646 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Animals Base Sequence Biological and medical sciences Blotting, Northern Chromosome Mapping Chromosomes, Human, Pair 16 - genetics Crosses, Genetic DNA Primers Exons Fundamental and applied biological sciences. Psychology Gene Library Genes. Genome GTP-Binding Proteins - genetics Humans Hypothalamus - cytology Hypothalamus - ultrastructure In Situ Hybridization In Situ Hybridization, Fluorescence Introns Metaphase Mice Mice, Inbred C57BL Models, Biological Molecular and cellular biology Molecular genetics Molecular Sequence Data Myocardium - cytology Myocardium - metabolism Myocardium - ultrastructure Rats Receptors, Cell Surface - genetics Receptors, G-Protein-Coupled Secretin - genetics Thyroid Gland - cytology Thyroid Gland - metabolism Tissue Distribution |
| title | GPR56, a Novel Secretin-like Human G-Protein-Coupled Receptor Gene |
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