Tear fluid analysis in patients with primary Sjögren's syndrome using lectin probes, A comparative study of patients with primary Sjögren's syndrome, patients with other immune inflammatory connective tissue diseases and controls
To study and compare the glycoprotein composition of tear fluid from patients with primary Sjögren's syndrome, patients with other connective tissue diseases and control individuals. SDS polyacrylamide gel electrophoresis followed by Coomassie staining or Western blotting and overlaying with te...
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| Veröffentlicht in: | Acta ophthalmologica Scandinavica 1999-02, Vol.77 (1), p.1-8 |
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| container_title | Acta ophthalmologica Scandinavica |
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| creator | Bjerrum, Kirsten B. |
| description | To study and compare the glycoprotein composition of tear fluid from patients with primary Sjögren's syndrome, patients with other connective tissue diseases and control individuals.
SDS polyacrylamide gel electrophoresis followed by Coomassie staining or Western blotting and overlaying with ten different lectins.
The frequency of many glycoprotein bands discovered was significantly decreased in primary Sjögren's syndrome compared with controls and patients with other connective tissue diseases. The reduction was most pronounced in the 14-68 kDa molecular weight range.
A decrease in the production rate and/or a change in the glycosylation pattern of the tear glycoproteins was found in patients with primary Sjögren's syndrome. The changes discovered, however, were not specific enough to be useful as a diagnostic test. |
| doi_str_mv | 10.1034/j.1600-0420.1999.770101.x |
| format | Article |
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SDS polyacrylamide gel electrophoresis followed by Coomassie staining or Western blotting and overlaying with ten different lectins.
The frequency of many glycoprotein bands discovered was significantly decreased in primary Sjögren's syndrome compared with controls and patients with other connective tissue diseases. The reduction was most pronounced in the 14-68 kDa molecular weight range.
A decrease in the production rate and/or a change in the glycosylation pattern of the tear glycoproteins was found in patients with primary Sjögren's syndrome. The changes discovered, however, were not specific enough to be useful as a diagnostic test.</description><identifier>ISSN: 1395-3907</identifier><identifier>EISSN: 1600-0420</identifier><identifier>DOI: 10.1034/j.1600-0420.1999.770101.x</identifier><identifier>PMID: 10071138</identifier><language>eng</language><publisher>Copenhagen, DK: Munksgaard International Publishers</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Blotting, Western ; Electrophoresis, Polyacrylamide Gel ; Eye Proteins - metabolism ; Female ; Glycoproteins - metabolism ; Humans ; Lectins - metabolism ; Male ; Middle Aged ; Molecular Weight ; Sjogren's Syndrome - metabolism ; Tears - metabolism</subject><ispartof>Acta ophthalmologica Scandinavica, 1999-02, Vol.77 (1), p.1-8</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3731-c6672f92e6da72e81ff67f79d2ceba123b81026cc1e71ec990148c7c52620f553</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1034%2Fj.1600-0420.1999.770101.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1034%2Fj.1600-0420.1999.770101.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27923,27924,45573,45574,46408,46832</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10071138$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bjerrum, Kirsten B.</creatorcontrib><title>Tear fluid analysis in patients with primary Sjögren's syndrome using lectin probes, A comparative study of patients with primary Sjögren's syndrome, patients with other immune inflammatory connective tissue diseases and controls</title><title>Acta ophthalmologica Scandinavica</title><addtitle>Acta Ophthalmol Scand</addtitle><description>To study and compare the glycoprotein composition of tear fluid from patients with primary Sjögren's syndrome, patients with other connective tissue diseases and control individuals.
SDS polyacrylamide gel electrophoresis followed by Coomassie staining or Western blotting and overlaying with ten different lectins.
The frequency of many glycoprotein bands discovered was significantly decreased in primary Sjögren's syndrome compared with controls and patients with other connective tissue diseases. The reduction was most pronounced in the 14-68 kDa molecular weight range.
A decrease in the production rate and/or a change in the glycosylation pattern of the tear glycoproteins was found in patients with primary Sjögren's syndrome. The changes discovered, however, were not specific enough to be useful as a diagnostic test.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Blotting, Western</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Eye Proteins - metabolism</subject><subject>Female</subject><subject>Glycoproteins - metabolism</subject><subject>Humans</subject><subject>Lectins - metabolism</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Molecular Weight</subject><subject>Sjogren's Syndrome - metabolism</subject><subject>Tears - metabolism</subject><issn>1395-3907</issn><issn>1600-0420</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNUU1u3CAUtqpGzU97hYpu2k08ATxjzHI0aptKkbJIukYMfiSMbJjycBJfLBfIYXqNYnlUVV1FQoLH-37gfUXxidEFo9XyYrdgNaUlXfJ8IaVcCEEZZYunN8XJ387bfK7kqqwkFcfFKeKOUippw98Vx4xSwVjVnBS_b0FHYrvBtUR73Y3okDhP9jo58AnJo0v3ZB9dr-NIbnYvz3cR_BckOPo2hh7IgM7fkQ5MmmgxbAHPyZqY0O91zCoPQDAN7UiCfb3q-X_QkO4hEtf3g4f8PNvpvtcpZLIJ3k_e2SY5xAFI6xA0Aub_tFM7xdDh--LI6g7hw2E_K35--3q7uSyvrr__2KyvSlOJipWmrgW3kkPdasGhYdbWwgrZcgNbzXi1bRjltTEMBAMjJWXLxgiz4jWndrWqzorPs26exK8BMKneoYGu0x7CgKqWNeNUygyUM9DEgBjBqsM4FKNqSlnt1JSlmrJUU8pqTlk9Ze7Hg8mw7aH9hznHmgGbGfDoOhhfr6zW1zd5zWX1B7NEwFc</recordid><startdate>199902</startdate><enddate>199902</enddate><creator>Bjerrum, Kirsten B.</creator><general>Munksgaard International Publishers</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199902</creationdate><title>Tear fluid analysis in patients with primary Sjögren's syndrome using lectin probes, A comparative study of patients with primary Sjögren's syndrome, patients with other immune inflammatory connective tissue diseases and controls</title><author>Bjerrum, Kirsten B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3731-c6672f92e6da72e81ff67f79d2ceba123b81026cc1e71ec990148c7c52620f553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Blotting, Western</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Eye Proteins - metabolism</topic><topic>Female</topic><topic>Glycoproteins - metabolism</topic><topic>Humans</topic><topic>Lectins - metabolism</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Molecular Weight</topic><topic>Sjogren's Syndrome - metabolism</topic><topic>Tears - metabolism</topic><toplevel>online_resources</toplevel><creatorcontrib>Bjerrum, Kirsten B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Acta ophthalmologica Scandinavica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bjerrum, Kirsten B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tear fluid analysis in patients with primary Sjögren's syndrome using lectin probes, A comparative study of patients with primary Sjögren's syndrome, patients with other immune inflammatory connective tissue diseases and controls</atitle><jtitle>Acta ophthalmologica Scandinavica</jtitle><addtitle>Acta Ophthalmol Scand</addtitle><date>1999-02</date><risdate>1999</risdate><volume>77</volume><issue>1</issue><spage>1</spage><epage>8</epage><pages>1-8</pages><issn>1395-3907</issn><eissn>1600-0420</eissn><abstract>To study and compare the glycoprotein composition of tear fluid from patients with primary Sjögren's syndrome, patients with other connective tissue diseases and control individuals.
SDS polyacrylamide gel electrophoresis followed by Coomassie staining or Western blotting and overlaying with ten different lectins.
The frequency of many glycoprotein bands discovered was significantly decreased in primary Sjögren's syndrome compared with controls and patients with other connective tissue diseases. The reduction was most pronounced in the 14-68 kDa molecular weight range.
A decrease in the production rate and/or a change in the glycosylation pattern of the tear glycoproteins was found in patients with primary Sjögren's syndrome. The changes discovered, however, were not specific enough to be useful as a diagnostic test.</abstract><cop>Copenhagen, DK</cop><pub>Munksgaard International Publishers</pub><pmid>10071138</pmid><doi>10.1034/j.1600-0420.1999.770101.x</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1395-3907 |
| ispartof | Acta ophthalmologica Scandinavica, 1999-02, Vol.77 (1), p.1-8 |
| issn | 1395-3907 1600-0420 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69612099 |
| source | MEDLINE; Wiley Free Content; Wiley Online Library All Journals |
| subjects | Adult Aged Aged, 80 and over Blotting, Western Electrophoresis, Polyacrylamide Gel Eye Proteins - metabolism Female Glycoproteins - metabolism Humans Lectins - metabolism Male Middle Aged Molecular Weight Sjogren's Syndrome - metabolism Tears - metabolism |
| title | Tear fluid analysis in patients with primary Sjögren's syndrome using lectin probes, A comparative study of patients with primary Sjögren's syndrome, patients with other immune inflammatory connective tissue diseases and controls |
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