HTLV-1 infection in blood donors from the Western Brazilian Amazon region: Seroprevalence and molecular study of viral isolates
To determine the seroprevalence of HTLV-1 in Brazil, and to review the virus molecular epidemiology in this Amazon population (Rio Branco-Acre), 219 blood donors were screened for HTLV-1. Only one case of infection (0.46% seroprevalence) was detected during July 2004 screening at the Acre Hospital F...
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container_end_page | 1971 |
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container_issue | 11 |
container_start_page | 1966 |
container_title | Journal of medical virology |
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creator | Mota-Miranda, Aline Cristina Araújo, Sérgio Pereira Dias, Juarez Pereira Colin, Denise Duizit Kashima, Simone Covas, Dimas Tadeu Tavares-Neto, José Galvão-Castro, Bernardo Alcantara, Luiz Carlos Junior |
description | To determine the seroprevalence of HTLV-1 in Brazil, and to review the virus molecular epidemiology in this Amazon population (Rio Branco-Acre), 219 blood donors were screened for HTLV-1. Only one case of infection (0.46% seroprevalence) was detected during July 2004 screening at the Acre Hospital Foundation (FUNDACRE). Neighbor-joining and Maximum Likelihood phylogenetic analyses of two (n = 2) complete LTR region sequences were performed with the PAUP* software. Since the HTLV-1 envelope surface (gp46) and transmembrane (gp21) glycoproteins are important for virus fitness, three envelope glycoproteins sequences (n = 3) were analyzed using the Prosite tool to determinate potential protein sites. Phylogenetic analysis demonstrated that the new isolate described in this study, and the unpublished LTR strain described in a previous report belong to the Transcontinental subgroup of the Cosmopolitan subtype, inside the Latin American cluster. A similar result was obtained when submitting, to the Automated Genotyping System, three LTR partial sequences from a previous study of the seroprevalence of HTLV-1 in the same Amazon population. In all analyzed env sequences, the potential protein site was found: two PKC phosphorylation sites at amino acid (aa) positions 310-312 and 342-344, one CK2 phosphorylation site at 194-197aa, three N-glycosylation sites at 222-225aa, 244-247aa and 272-275aa, and a single N-myristylation site at 327-338aa. In conclusion, potential protein sites described in HTLV-1 gp46 and gp21 confirm the presence of conserved sites in the HTLV-1 envelope proteins, likewise phylogenetic analysis suggests a possible recent introduction of the virus into North Brazil. J. Med. Virol. 80:1966-1971, 2008. |
doi_str_mv | 10.1002/jmv.21300 |
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Only one case of infection (0.46% seroprevalence) was detected during July 2004 screening at the Acre Hospital Foundation (FUNDACRE). Neighbor-joining and Maximum Likelihood phylogenetic analyses of two (n = 2) complete LTR region sequences were performed with the PAUP* software. Since the HTLV-1 envelope surface (gp46) and transmembrane (gp21) glycoproteins are important for virus fitness, three envelope glycoproteins sequences (n = 3) were analyzed using the Prosite tool to determinate potential protein sites. Phylogenetic analysis demonstrated that the new isolate described in this study, and the unpublished LTR strain described in a previous report belong to the Transcontinental subgroup of the Cosmopolitan subtype, inside the Latin American cluster. A similar result was obtained when submitting, to the Automated Genotyping System, three LTR partial sequences from a previous study of the seroprevalence of HTLV-1 in the same Amazon population. In all analyzed env sequences, the potential protein site was found: two PKC phosphorylation sites at amino acid (aa) positions 310-312 and 342-344, one CK2 phosphorylation site at 194-197aa, three N-glycosylation sites at 222-225aa, 244-247aa and 272-275aa, and a single N-myristylation site at 327-338aa. In conclusion, potential protein sites described in HTLV-1 gp46 and gp21 confirm the presence of conserved sites in the HTLV-1 envelope proteins, likewise phylogenetic analysis suggests a possible recent introduction of the virus into North Brazil. J. Med. Virol. 80:1966-1971, 2008.</description><identifier>ISSN: 0146-6615</identifier><identifier>EISSN: 1096-9071</identifier><identifier>DOI: 10.1002/jmv.21300</identifier><identifier>PMID: 18814249</identifier><identifier>CODEN: JMVIDB</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Biological and medical sciences ; Blood Donors ; Brazil - epidemiology ; Cluster Analysis ; Conserved Sequence ; Epidemiology ; Fundamental and applied biological sciences. Psychology ; Gene Products, env - genetics ; Genotype ; glycoproteins ; HTLV-I Infections - epidemiology ; Human T-lymphotropic virus 1 ; Human T-lymphotropic virus 1 - classification ; Human T-lymphotropic virus 1 - genetics ; Human T-lymphotropic virus 1 - isolation & purification ; Human viral diseases ; Humans ; Infectious diseases ; LTR region ; Medical sciences ; Microbiology ; Miscellaneous ; phylogenetic analysis ; Phylogeny ; protein sites ; RNA, Viral - genetics ; Sequence Analysis, DNA ; Sequence Homology ; Seroepidemiologic Studies ; Viral diseases ; Virology</subject><ispartof>Journal of medical virology, 2008-11, Vol.80 (11), p.1966-1971</ispartof><rights>Copyright © 2008 Wiley‐Liss, Inc.</rights><rights>2008 INIST-CNRS</rights><rights>2008 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4810-e2647c1c92ee3e1c675b3c820133ff7f1a0c1c379253794a5901422e79e02aad3</citedby><cites>FETCH-LOGICAL-c4810-e2647c1c92ee3e1c675b3c820133ff7f1a0c1c379253794a5901422e79e02aad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjmv.21300$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjmv.21300$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20696218$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18814249$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mota-Miranda, Aline Cristina</creatorcontrib><creatorcontrib>Araújo, Sérgio Pereira</creatorcontrib><creatorcontrib>Dias, Juarez Pereira</creatorcontrib><creatorcontrib>Colin, Denise Duizit</creatorcontrib><creatorcontrib>Kashima, Simone</creatorcontrib><creatorcontrib>Covas, Dimas Tadeu</creatorcontrib><creatorcontrib>Tavares-Neto, José</creatorcontrib><creatorcontrib>Galvão-Castro, Bernardo</creatorcontrib><creatorcontrib>Alcantara, Luiz Carlos Junior</creatorcontrib><title>HTLV-1 infection in blood donors from the Western Brazilian Amazon region: Seroprevalence and molecular study of viral isolates</title><title>Journal of medical virology</title><addtitle>J. Med. Virol</addtitle><description>To determine the seroprevalence of HTLV-1 in Brazil, and to review the virus molecular epidemiology in this Amazon population (Rio Branco-Acre), 219 blood donors were screened for HTLV-1. Only one case of infection (0.46% seroprevalence) was detected during July 2004 screening at the Acre Hospital Foundation (FUNDACRE). Neighbor-joining and Maximum Likelihood phylogenetic analyses of two (n = 2) complete LTR region sequences were performed with the PAUP* software. Since the HTLV-1 envelope surface (gp46) and transmembrane (gp21) glycoproteins are important for virus fitness, three envelope glycoproteins sequences (n = 3) were analyzed using the Prosite tool to determinate potential protein sites. Phylogenetic analysis demonstrated that the new isolate described in this study, and the unpublished LTR strain described in a previous report belong to the Transcontinental subgroup of the Cosmopolitan subtype, inside the Latin American cluster. A similar result was obtained when submitting, to the Automated Genotyping System, three LTR partial sequences from a previous study of the seroprevalence of HTLV-1 in the same Amazon population. In all analyzed env sequences, the potential protein site was found: two PKC phosphorylation sites at amino acid (aa) positions 310-312 and 342-344, one CK2 phosphorylation site at 194-197aa, three N-glycosylation sites at 222-225aa, 244-247aa and 272-275aa, and a single N-myristylation site at 327-338aa. In conclusion, potential protein sites described in HTLV-1 gp46 and gp21 confirm the presence of conserved sites in the HTLV-1 envelope proteins, likewise phylogenetic analysis suggests a possible recent introduction of the virus into North Brazil. J. Med. Virol. 80:1966-1971, 2008.</description><subject>Biological and medical sciences</subject><subject>Blood Donors</subject><subject>Brazil - epidemiology</subject><subject>Cluster Analysis</subject><subject>Conserved Sequence</subject><subject>Epidemiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Products, env - genetics</subject><subject>Genotype</subject><subject>glycoproteins</subject><subject>HTLV-I Infections - epidemiology</subject><subject>Human T-lymphotropic virus 1</subject><subject>Human T-lymphotropic virus 1 - classification</subject><subject>Human T-lymphotropic virus 1 - genetics</subject><subject>Human T-lymphotropic virus 1 - isolation & purification</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>LTR region</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>phylogenetic analysis</subject><subject>Phylogeny</subject><subject>protein sites</subject><subject>RNA, Viral - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology</subject><subject>Seroepidemiologic Studies</subject><subject>Viral diseases</subject><subject>Virology</subject><issn>0146-6615</issn><issn>1096-9071</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1vEzEQhlcIREPhwB8AX0DisK3H3i9zKxWkVAEESVtu1sQ7W1y862AngfTCX8cloZwQF4-led75erPsMfAD4FwcXvXrAwGS8zvZCLiqcsVruJuNOBRVXlVQ7mUPYrzinDdKiPvZHjQNFKJQo-znyWxyngOzQ0dmaf2QfmzuvG9Z6wcfIuuC79nyC7ELiksKA3sV8No6iwM76vE6KQJdJuFLNqXgF4HW6GgwxHBoWe8dmZXDwOJy1W6Y79jaBnTMRu9wSfFhdq9DF-nRLu5nZ29ez45P8smH8dvjo0luigZ4TqIqagNGCSJJYKq6nEvTCA5Sdl3dAfKUlbUSZXoKLFXaXQiqFXGB2Mr97Pm27iL4b6u0ie5tNOQcDuRXUVeqAg5J_T9QcFE2dS0T-GILmuBjDNTpRbA9ho0Grm9s0ckW_duWxD7ZFV3Ne2r_kjsfEvBsB2A06LqAg7HxlhM8zSegSdzhlvtuHW3-3VGfvjv_0zrfKmxy78etAsNXXdWyLvXF-7H-rMYfTz_BVM8S_3TLd-g1XoY0xdn05s4cyqJRspS_AGcQvD8</recordid><startdate>200811</startdate><enddate>200811</enddate><creator>Mota-Miranda, Aline Cristina</creator><creator>Araújo, Sérgio Pereira</creator><creator>Dias, Juarez Pereira</creator><creator>Colin, Denise Duizit</creator><creator>Kashima, Simone</creator><creator>Covas, Dimas Tadeu</creator><creator>Tavares-Neto, José</creator><creator>Galvão-Castro, Bernardo</creator><creator>Alcantara, Luiz Carlos Junior</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200811</creationdate><title>HTLV-1 infection in blood donors from the Western Brazilian Amazon region: Seroprevalence and molecular study of viral isolates</title><author>Mota-Miranda, Aline Cristina ; Araújo, Sérgio Pereira ; Dias, Juarez Pereira ; Colin, Denise Duizit ; Kashima, Simone ; Covas, Dimas Tadeu ; Tavares-Neto, José ; Galvão-Castro, Bernardo ; Alcantara, Luiz Carlos Junior</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4810-e2647c1c92ee3e1c675b3c820133ff7f1a0c1c379253794a5901422e79e02aad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Biological and medical sciences</topic><topic>Blood Donors</topic><topic>Brazil - epidemiology</topic><topic>Cluster Analysis</topic><topic>Conserved Sequence</topic><topic>Epidemiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Products, env - genetics</topic><topic>Genotype</topic><topic>glycoproteins</topic><topic>HTLV-I Infections - epidemiology</topic><topic>Human T-lymphotropic virus 1</topic><topic>Human T-lymphotropic virus 1 - classification</topic><topic>Human T-lymphotropic virus 1 - genetics</topic><topic>Human T-lymphotropic virus 1 - isolation & purification</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>LTR region</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>phylogenetic analysis</topic><topic>Phylogeny</topic><topic>protein sites</topic><topic>RNA, Viral - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology</topic><topic>Seroepidemiologic Studies</topic><topic>Viral diseases</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mota-Miranda, Aline Cristina</creatorcontrib><creatorcontrib>Araújo, Sérgio Pereira</creatorcontrib><creatorcontrib>Dias, Juarez Pereira</creatorcontrib><creatorcontrib>Colin, Denise Duizit</creatorcontrib><creatorcontrib>Kashima, Simone</creatorcontrib><creatorcontrib>Covas, Dimas Tadeu</creatorcontrib><creatorcontrib>Tavares-Neto, José</creatorcontrib><creatorcontrib>Galvão-Castro, Bernardo</creatorcontrib><creatorcontrib>Alcantara, Luiz Carlos Junior</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of medical virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mota-Miranda, Aline Cristina</au><au>Araújo, Sérgio Pereira</au><au>Dias, Juarez Pereira</au><au>Colin, Denise Duizit</au><au>Kashima, Simone</au><au>Covas, Dimas Tadeu</au><au>Tavares-Neto, José</au><au>Galvão-Castro, Bernardo</au><au>Alcantara, Luiz Carlos Junior</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HTLV-1 infection in blood donors from the Western Brazilian Amazon region: Seroprevalence and molecular study of viral isolates</atitle><jtitle>Journal of medical virology</jtitle><addtitle>J. Med. Virol</addtitle><date>2008-11</date><risdate>2008</risdate><volume>80</volume><issue>11</issue><spage>1966</spage><epage>1971</epage><pages>1966-1971</pages><issn>0146-6615</issn><eissn>1096-9071</eissn><coden>JMVIDB</coden><abstract>To determine the seroprevalence of HTLV-1 in Brazil, and to review the virus molecular epidemiology in this Amazon population (Rio Branco-Acre), 219 blood donors were screened for HTLV-1. Only one case of infection (0.46% seroprevalence) was detected during July 2004 screening at the Acre Hospital Foundation (FUNDACRE). Neighbor-joining and Maximum Likelihood phylogenetic analyses of two (n = 2) complete LTR region sequences were performed with the PAUP* software. Since the HTLV-1 envelope surface (gp46) and transmembrane (gp21) glycoproteins are important for virus fitness, three envelope glycoproteins sequences (n = 3) were analyzed using the Prosite tool to determinate potential protein sites. Phylogenetic analysis demonstrated that the new isolate described in this study, and the unpublished LTR strain described in a previous report belong to the Transcontinental subgroup of the Cosmopolitan subtype, inside the Latin American cluster. A similar result was obtained when submitting, to the Automated Genotyping System, three LTR partial sequences from a previous study of the seroprevalence of HTLV-1 in the same Amazon population. In all analyzed env sequences, the potential protein site was found: two PKC phosphorylation sites at amino acid (aa) positions 310-312 and 342-344, one CK2 phosphorylation site at 194-197aa, three N-glycosylation sites at 222-225aa, 244-247aa and 272-275aa, and a single N-myristylation site at 327-338aa. In conclusion, potential protein sites described in HTLV-1 gp46 and gp21 confirm the presence of conserved sites in the HTLV-1 envelope proteins, likewise phylogenetic analysis suggests a possible recent introduction of the virus into North Brazil. J. Med. Virol. 80:1966-1971, 2008.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>18814249</pmid><doi>10.1002/jmv.21300</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Biological and medical sciences Blood Donors Brazil - epidemiology Cluster Analysis Conserved Sequence Epidemiology Fundamental and applied biological sciences. Psychology Gene Products, env - genetics Genotype glycoproteins HTLV-I Infections - epidemiology Human T-lymphotropic virus 1 Human T-lymphotropic virus 1 - classification Human T-lymphotropic virus 1 - genetics Human T-lymphotropic virus 1 - isolation & purification Human viral diseases Humans Infectious diseases LTR region Medical sciences Microbiology Miscellaneous phylogenetic analysis Phylogeny protein sites RNA, Viral - genetics Sequence Analysis, DNA Sequence Homology Seroepidemiologic Studies Viral diseases Virology |
title | HTLV-1 infection in blood donors from the Western Brazilian Amazon region: Seroprevalence and molecular study of viral isolates |
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