CREB-binding [corrected] protein interacts with the homeodomain protein Cdx2 and enhances transcriptional activity
Cdx2 encodes for a homeodomain protein that is expressed in intestinal epithelial cells. The Cdx2 protein triggers intestinal differentiation in cell lines and is necessary for maintenance of the intestinal phenotype in mice. CBP (cAMP response element-binding protein) is a transcriptional co-activa...
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| Veröffentlicht in: | The Journal of biological chemistry 1999-03, Vol.274 (11), p.7196-7199 |
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| container_issue | 11 |
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| container_title | The Journal of biological chemistry |
| container_volume | 274 |
| creator | Lorentz, O Suh, E R Taylor, J K Boudreau, F Traber, P G |
| description | Cdx2 encodes for a homeodomain protein that is expressed in intestinal epithelial cells. The Cdx2 protein triggers intestinal differentiation in cell lines and is necessary for maintenance of the intestinal phenotype in mice. CBP (cAMP response element-binding protein) is a transcriptional co-activator that interacts with many transcription factors and components of the basal transcriptional machinery. In this study, we demonstrate that CBP is markedly induced upon differentiation of the Caco-2 intestinal cell line and augments Cdx2-dependent transcriptional activity. Cdx2 interacts with the amino-terminal domain of CBP, and the two proteins coexist in vivo within the same nuclear protein complex. Moreover, expression of the CBP domain that interacts with Cdx2 acts as a dominant-negative inhibitor of transcriptional activation by Cdx2. These findings demonstrate a direct interaction between an intestinal homeodomain protein and CBP and suggest that CBP participates in the network of transcriptional proteins that direct intestinal differentiation. |
| format | Article |
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The Cdx2 protein triggers intestinal differentiation in cell lines and is necessary for maintenance of the intestinal phenotype in mice. CBP (cAMP response element-binding protein) is a transcriptional co-activator that interacts with many transcription factors and components of the basal transcriptional machinery. In this study, we demonstrate that CBP is markedly induced upon differentiation of the Caco-2 intestinal cell line and augments Cdx2-dependent transcriptional activity. Cdx2 interacts with the amino-terminal domain of CBP, and the two proteins coexist in vivo within the same nuclear protein complex. Moreover, expression of the CBP domain that interacts with Cdx2 acts as a dominant-negative inhibitor of transcriptional activation by Cdx2. 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The Cdx2 protein triggers intestinal differentiation in cell lines and is necessary for maintenance of the intestinal phenotype in mice. CBP (cAMP response element-binding protein) is a transcriptional co-activator that interacts with many transcription factors and components of the basal transcriptional machinery. In this study, we demonstrate that CBP is markedly induced upon differentiation of the Caco-2 intestinal cell line and augments Cdx2-dependent transcriptional activity. Cdx2 interacts with the amino-terminal domain of CBP, and the two proteins coexist in vivo within the same nuclear protein complex. Moreover, expression of the CBP domain that interacts with Cdx2 acts as a dominant-negative inhibitor of transcriptional activation by Cdx2. These findings demonstrate a direct interaction between an intestinal homeodomain protein and CBP and suggest that CBP participates in the network of transcriptional proteins that direct intestinal differentiation.</description><subject>3T3 Cells</subject><subject>Animals</subject><subject>Caco-2 Cells</subject><subject>CCAAT-Enhancer-Binding Proteins</subject><subject>Cell Differentiation</subject><subject>Cell Division</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Humans</subject><subject>Mice</subject><subject>Nuclear Proteins - metabolism</subject><subject>Protein Binding</subject><subject>Transcription, Genetic</subject><issn>0021-9258</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kE9LxDAQxXNQ3HX1K0hO3gpJmrbpUcv6BxYE2ZtISZOpjbRJTVJ1v70Bd-fwZmB-7zHMGVoTwmhWs0Ks0GUInyQVr-kFWlFCyrISZI1887q9zzpjtbEf-E0570FF0O949i6CsdjYCF6qGPCPiQOOA-DBTeC0m2Ran7BG_zIsrcZgB2kVBBy9tEF5M0fjrBxxyjDfJh6u0HkvxwDXx75B-4ftvnnKdi-Pz83dLpsLTrKOVJXQRDAgAqBntC4qxUhSyss0adr3dadECSpXrCoIz4FxnTw5p1R2-Qbd_semC78WCLGdTFAwjtKCW0Jb1iWpKOUJvDmCSzeBbmdvJukP7elJ-R_sUmOc</recordid><startdate>19990312</startdate><enddate>19990312</enddate><creator>Lorentz, O</creator><creator>Suh, E R</creator><creator>Taylor, J K</creator><creator>Boudreau, F</creator><creator>Traber, P G</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19990312</creationdate><title>CREB-binding [corrected] protein interacts with the homeodomain protein Cdx2 and enhances transcriptional activity</title><author>Lorentz, O ; Suh, E R ; Taylor, J K ; Boudreau, F ; Traber, P G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p540-b0778d082e08eef21957c209571467c2d1ff9bc86ec3c275043e24d78d3411ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>3T3 Cells</topic><topic>Animals</topic><topic>Caco-2 Cells</topic><topic>CCAAT-Enhancer-Binding Proteins</topic><topic>Cell Differentiation</topic><topic>Cell Division</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Humans</topic><topic>Mice</topic><topic>Nuclear Proteins - metabolism</topic><topic>Protein Binding</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lorentz, O</creatorcontrib><creatorcontrib>Suh, E R</creatorcontrib><creatorcontrib>Taylor, J K</creatorcontrib><creatorcontrib>Boudreau, F</creatorcontrib><creatorcontrib>Traber, P G</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lorentz, O</au><au>Suh, E R</au><au>Taylor, J K</au><au>Boudreau, F</au><au>Traber, P G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CREB-binding [corrected] protein interacts with the homeodomain protein Cdx2 and enhances transcriptional activity</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1999-03-12</date><risdate>1999</risdate><volume>274</volume><issue>11</issue><spage>7196</spage><epage>7199</epage><pages>7196-7199</pages><issn>0021-9258</issn><abstract>Cdx2 encodes for a homeodomain protein that is expressed in intestinal epithelial cells. The Cdx2 protein triggers intestinal differentiation in cell lines and is necessary for maintenance of the intestinal phenotype in mice. CBP (cAMP response element-binding protein) is a transcriptional co-activator that interacts with many transcription factors and components of the basal transcriptional machinery. In this study, we demonstrate that CBP is markedly induced upon differentiation of the Caco-2 intestinal cell line and augments Cdx2-dependent transcriptional activity. Cdx2 interacts with the amino-terminal domain of CBP, and the two proteins coexist in vivo within the same nuclear protein complex. Moreover, expression of the CBP domain that interacts with Cdx2 acts as a dominant-negative inhibitor of transcriptional activation by Cdx2. 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| ispartof | The Journal of biological chemistry, 1999-03, Vol.274 (11), p.7196-7199 |
| issn | 0021-9258 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | 3T3 Cells Animals Caco-2 Cells CCAAT-Enhancer-Binding Proteins Cell Differentiation Cell Division DNA-Binding Proteins - metabolism Homeodomain Proteins - metabolism Humans Mice Nuclear Proteins - metabolism Protein Binding Transcription, Genetic |
| title | CREB-binding [corrected] protein interacts with the homeodomain protein Cdx2 and enhances transcriptional activity |
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