The Matrix Metalloprotease Pump-1 (MMP-7, Matrilysin): A Candidate Marker/Target for Ovarian Cancer Detection and Treatment
Matrix metalloproteases are known to play an important role in tumor invasion by mediating degradation of extracellular matrix. In this study, we have investigated the expression of the matrix metalloprotease pump-1 gene (also referred to as MMP-7, Matrilysin) in 44 ovarian tumors (12 low malignant...
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| Veröffentlicht in: | Tumor biology 1999-03, Vol.20 (2), p.88-98 |
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| container_title | Tumor biology |
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| creator | Tanimoto, Hirotoshi Underwood, Lowell J. Shigemasa, Kazushi Parmley, Tim H. Wang, Yinxiang Yan, Yan Clarke, John O’Brien, Timothy J. |
| description | Matrix metalloproteases are known to play an important role in tumor invasion by mediating degradation of extracellular matrix. In this study, we have investigated the expression of the matrix metalloprotease pump-1 gene (also referred to as MMP-7, Matrilysin) in 44 ovarian tumors (12 low malignant potential tumors, 32 carcinomas) and 10 normal ovaries using quantitative PCR. The PCR product was labelled with 32 P and a phosphoimager was used to determine the relative expression of pump-1 compared to an internal control β-tubulin. mRNA expression levels of pump-1 were significantly elevated in 9 of 12 low malignant potential tumors and 26 of 32 carcinomas. Northern blot hybridization showed that the 1.1-kb pump-1 transcript was abundant in carcinoma but seldom expressed in normal adult tissues including normal ovary. Immunohistochemical localization of the pump-1 protein confirms its expression by ovarian tumor cells. Our results suggest that pump-1 is frequently overexpressed in ovarian tumors and may contribute to its invasive nature or growth capacity, therefore pump-1 may serve as a useful marker for early detection of disease and/or a target for therapeutic intervention in downregulation of tumor progression. |
| doi_str_mv | 10.1159/000030051 |
| format | Article |
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In this study, we have investigated the expression of the matrix metalloprotease pump-1 gene (also referred to as MMP-7, Matrilysin) in 44 ovarian tumors (12 low malignant potential tumors, 32 carcinomas) and 10 normal ovaries using quantitative PCR. The PCR product was labelled with 32 P and a phosphoimager was used to determine the relative expression of pump-1 compared to an internal control β-tubulin. mRNA expression levels of pump-1 were significantly elevated in 9 of 12 low malignant potential tumors and 26 of 32 carcinomas. Northern blot hybridization showed that the 1.1-kb pump-1 transcript was abundant in carcinoma but seldom expressed in normal adult tissues including normal ovary. Immunohistochemical localization of the pump-1 protein confirms its expression by ovarian tumor cells. Our results suggest that pump-1 is frequently overexpressed in ovarian tumors and may contribute to its invasive nature or growth capacity, therefore pump-1 may serve as a useful marker for early detection of disease and/or a target for therapeutic intervention in downregulation of tumor progression.</description><identifier>ISSN: 1010-4283</identifier><identifier>EISSN: 1423-0380</identifier><identifier>DOI: 10.1159/000030051</identifier><identifier>PMID: 10050107</identifier><identifier>CODEN: OBIMD4</identifier><language>eng</language><publisher>Basel, Switzerland: Karger</publisher><subject>Biological and medical sciences ; Biomarkers, Tumor - metabolism ; Blotting, Northern ; Female ; Female genital diseases ; Gynecology. Andrology. Obstetrics ; Humans ; Immunohistochemistry ; Matrix Metalloproteinase 7 ; Medical sciences ; Metalloendopeptidases - genetics ; Metalloendopeptidases - metabolism ; Original Paper ; Ovarian Neoplasms - enzymology ; Ovarian Neoplasms - pathology ; Ovary - enzymology ; Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Tumors</subject><ispartof>Tumor biology, 1999-03, Vol.20 (2), p.88-98</ispartof><rights>1999 S. Karger AG, Basel</rights><rights>1999 INIST-CNRS</rights><rights>Copyright (c) 1999 S. 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In this study, we have investigated the expression of the matrix metalloprotease pump-1 gene (also referred to as MMP-7, Matrilysin) in 44 ovarian tumors (12 low malignant potential tumors, 32 carcinomas) and 10 normal ovaries using quantitative PCR. The PCR product was labelled with 32 P and a phosphoimager was used to determine the relative expression of pump-1 compared to an internal control β-tubulin. mRNA expression levels of pump-1 were significantly elevated in 9 of 12 low malignant potential tumors and 26 of 32 carcinomas. Northern blot hybridization showed that the 1.1-kb pump-1 transcript was abundant in carcinoma but seldom expressed in normal adult tissues including normal ovary. Immunohistochemical localization of the pump-1 protein confirms its expression by ovarian tumor cells. Our results suggest that pump-1 is frequently overexpressed in ovarian tumors and may contribute to its invasive nature or growth capacity, therefore pump-1 may serve as a useful marker for early detection of disease and/or a target for therapeutic intervention in downregulation of tumor progression.</description><subject>Biological and medical sciences</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Blotting, Northern</subject><subject>Female</subject><subject>Female genital diseases</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Matrix Metalloproteinase 7</subject><subject>Medical sciences</subject><subject>Metalloendopeptidases - genetics</subject><subject>Metalloendopeptidases - metabolism</subject><subject>Original Paper</subject><subject>Ovarian Neoplasms - enzymology</subject><subject>Ovarian Neoplasms - pathology</subject><subject>Ovary - enzymology</subject><subject>Polymerase Chain Reaction</subject><subject>RNA, Messenger - metabolism</subject><subject>Tumors</subject><issn>1010-4283</issn><issn>1423-0380</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNpt0c1LHTEQAPBQlPrRHnouSBARhW5NsnnZpDd9flTwoYfteclmJ-3qfrwmWVH85812H1rEXBKY32QmE4S-UPKd0pk6InGlhMzoB7RJOUsTkkqyFs-EkoQzmW6gLe9vCYlYiY9og0YcY9kmesr_AF7o4OoHvICgm6Zfuj6A9oBvhnaZUHywWNwk2bdJNY--7g5_4GM8111VVzqM6e4O3FGu3W8I2PYOX99rV-tuNAYcPoUAJtR9h2MOzh3o0EIXPqF1qxsPn1f7Nvp1fpbPfyZX1xeX8-OrxKSSh6RSwEHbTFnGKy5KW5aVIGCYYixjFNSMMiszaUshLRWZzDhVzPBMkcpYydJttD_dG1_2dwAfirb2BppGd9APvhBKECqJinD3DbztB9fF3go21hIsHdHhhIzrvXdgi6WrW-0eC0qK8TuKl--Idmd14VC2UP0np_lHsLcC2hvdWBcHVvtXJ5Riir82djfO2L3E85PLf4WKZWUj-voumlp5BrFao58</recordid><startdate>19990301</startdate><enddate>19990301</enddate><creator>Tanimoto, Hirotoshi</creator><creator>Underwood, Lowell J.</creator><creator>Shigemasa, Kazushi</creator><creator>Parmley, Tim H.</creator><creator>Wang, Yinxiang</creator><creator>Yan, Yan</creator><creator>Clarke, John</creator><creator>O’Brien, Timothy J.</creator><general>Karger</general><general>Springer Nature B.V</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>19990301</creationdate><title>The Matrix Metalloprotease Pump-1 (MMP-7, Matrilysin): A Candidate Marker/Target for Ovarian Cancer Detection and Treatment</title><author>Tanimoto, Hirotoshi ; Underwood, Lowell J. ; Shigemasa, Kazushi ; Parmley, Tim H. ; Wang, Yinxiang ; Yan, Yan ; Clarke, John ; O’Brien, Timothy J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-d9e4eaf79f24d46bfbbd60ec2922721e9512f878fb68f167874192c4790dcf823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Biological and medical sciences</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Blotting, Northern</topic><topic>Female</topic><topic>Female genital diseases</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Matrix Metalloproteinase 7</topic><topic>Medical sciences</topic><topic>Metalloendopeptidases - genetics</topic><topic>Metalloendopeptidases - metabolism</topic><topic>Original Paper</topic><topic>Ovarian Neoplasms - enzymology</topic><topic>Ovarian Neoplasms - pathology</topic><topic>Ovary - enzymology</topic><topic>Polymerase Chain Reaction</topic><topic>RNA, Messenger - metabolism</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tanimoto, Hirotoshi</creatorcontrib><creatorcontrib>Underwood, Lowell J.</creatorcontrib><creatorcontrib>Shigemasa, Kazushi</creatorcontrib><creatorcontrib>Parmley, Tim H.</creatorcontrib><creatorcontrib>Wang, Yinxiang</creatorcontrib><creatorcontrib>Yan, Yan</creatorcontrib><creatorcontrib>Clarke, John</creatorcontrib><creatorcontrib>O’Brien, Timothy J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Tumor biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tanimoto, Hirotoshi</au><au>Underwood, Lowell J.</au><au>Shigemasa, Kazushi</au><au>Parmley, Tim H.</au><au>Wang, Yinxiang</au><au>Yan, Yan</au><au>Clarke, John</au><au>O’Brien, Timothy J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Matrix Metalloprotease Pump-1 (MMP-7, Matrilysin): A Candidate Marker/Target for Ovarian Cancer Detection and Treatment</atitle><jtitle>Tumor biology</jtitle><addtitle>Tumor Biol</addtitle><date>1999-03-01</date><risdate>1999</risdate><volume>20</volume><issue>2</issue><spage>88</spage><epage>98</epage><pages>88-98</pages><issn>1010-4283</issn><eissn>1423-0380</eissn><coden>OBIMD4</coden><abstract>Matrix metalloproteases are known to play an important role in tumor invasion by mediating degradation of extracellular matrix. In this study, we have investigated the expression of the matrix metalloprotease pump-1 gene (also referred to as MMP-7, Matrilysin) in 44 ovarian tumors (12 low malignant potential tumors, 32 carcinomas) and 10 normal ovaries using quantitative PCR. The PCR product was labelled with 32 P and a phosphoimager was used to determine the relative expression of pump-1 compared to an internal control β-tubulin. mRNA expression levels of pump-1 were significantly elevated in 9 of 12 low malignant potential tumors and 26 of 32 carcinomas. Northern blot hybridization showed that the 1.1-kb pump-1 transcript was abundant in carcinoma but seldom expressed in normal adult tissues including normal ovary. Immunohistochemical localization of the pump-1 protein confirms its expression by ovarian tumor cells. Our results suggest that pump-1 is frequently overexpressed in ovarian tumors and may contribute to its invasive nature or growth capacity, therefore pump-1 may serve as a useful marker for early detection of disease and/or a target for therapeutic intervention in downregulation of tumor progression.</abstract><cop>Basel, Switzerland</cop><pub>Karger</pub><pmid>10050107</pmid><doi>10.1159/000030051</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Tumor biology, 1999-03, Vol.20 (2), p.88-98 |
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| source | Karger Journals; MEDLINE |
| subjects | Biological and medical sciences Biomarkers, Tumor - metabolism Blotting, Northern Female Female genital diseases Gynecology. Andrology. Obstetrics Humans Immunohistochemistry Matrix Metalloproteinase 7 Medical sciences Metalloendopeptidases - genetics Metalloendopeptidases - metabolism Original Paper Ovarian Neoplasms - enzymology Ovarian Neoplasms - pathology Ovary - enzymology Polymerase Chain Reaction RNA, Messenger - metabolism Tumors |
| title | The Matrix Metalloprotease Pump-1 (MMP-7, Matrilysin): A Candidate Marker/Target for Ovarian Cancer Detection and Treatment |
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