Protein Purification from Polyacrylamide Gels by Sonication Extraction

A proteinpurification procedure using sonication extraction from polyacrylamide gels (PAGE), which involves identification of a particular protein band and its excision, homogenization, sonication, and subsequent passage through a Sephadex G-25 minicolumn, is reported. Our results show a high degree...

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Veröffentlicht in:	Analytical biochemistry 1999-03, Vol.268 (1), p.15-20
Hauptverfasser:	Retamal, Claudio A., Thiebaut, Paula, Alves, Elias W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Electrophoresis, Polyacrylamide Gel - methods gel electrophoresis Horses Male Membrane Proteins - isolation & purification native proteins protein purification proteins Proteins - isolation & purification Sonication sperm proteins Spermatozoa - chemistry
Online-Zugang:	Volltext
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