Observation of a Novel Transient Ferryl Complex with Reduced CuB in Cytochrome c Oxidase

The reaction between mixed-valence (MV) cytochrome c oxidase from beef heart with H2O2 was investigated using the flow-flash technique with a high concentration of H2O2 (1 M) to ensure a fast bimolecular interaction with the enzyme. Under anaerobic conditions the reaction exhibits 3 apparent phases....

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Veröffentlicht in:	Biochemistry (Easton) 1999-02, Vol.38 (8), p.2307-2311
Hauptverfasser:	Zaslavsky, Dmitry, Smirnova, Irina A, Ädelroth, Pia, Brzezinski, Peter, Gennis, Robert B
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cattle Copper - chemistry Electron Transport Electron Transport Complex IV - chemistry Heme - analogs & derivatives Heme - chemistry Hydrogen Peroxide - chemistry Iron - chemistry Kinetics Oxidation-Reduction Oxygen - chemistry Protons
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