Purification of monoclonal antibodies from cell culture supernatants using a modified zirconia based cation-exchange support

A method suitable for the isolation of monoclonal antibodies (Mabs) is described. The protocol utilizes a zirconia based column modified with ethylenediamine- N, N′-tetra(methylenephosphonic) acid to create a novel cation-exchange chromatographic support. Initial experiments using a linear salt grad...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of Chromatography A 1999-01, Vol.831 (1), p.63-72
Hauptverfasser:	Clausen, Andrew M, Subramanian, Anuradha, Carr, Peter W
Format:	Artikel
Sprache:	eng
Schlagworte:	Adsorption Antibodies, Monoclonal - isolation & purification Biological and medical sciences Biotechnology Cations Cells, Cultured Chelating Agents - pharmacology Chromatography, Ion Exchange - methods Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Health. Pharmaceutical industry Industrial applications and implications. Economical aspects Monoclonal antibodies Organophosphorus Compounds - pharmacology Production of active biomolecules Serum Albumin, Bovine - immunology Transferrin - immunology Zirconium
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!