Highly sensitive detection of swine vesicular disease virus based on a single tube RT-PCR system and DIG-ELISA detection

A highly sensitive detection of swine vesicular disease virus (SVDV) based on a single tube RT-PCR system and digoxigenin (DIG)-PCR-ELISA detection was developed. Using a one tube RT-PCR system, optimisation of the PCR conditions and optimisation of the microwell hybridisation and colourimetric dete...

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Veröffentlicht in:	Journal of virological methods 1999, Vol.77 (1), p.87-99
Hauptverfasser:	Callens, M, De Clercq, K
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Cells, Cultured Digoxigenin DNA Probes Enterovirus - genetics Enterovirus - growth & development Enterovirus - isolation & purification Enzyme-Linked Immunosorbent Assay - methods Epithelial Cells Faecal samples Feces - virology Foot-and-mouth disease virus Fundamental and applied biological sciences. Psychology Microbiology Reverse Transcriptase Polymerase Chain Reaction - methods RT-PCR-ELISA detection Sensitivity and Specificity SVDV diagnosis Swine Swine Vesicular Disease - diagnosis Swine Vesicular Disease - virology Swine vesicular disease virus Techniques used in virology Virology Virus Cultivation
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