Determination of angiogenic factors in serum by protein array in patients with renal cell carcinoma
Using the protein array method we determined the serum levels of a number of angiogenic factors. We identified serum levels of angiogenin, PDGF and MCP-1 (CCL2 chemokine) in serum of 32 patients with RCC, and 14 healthy volunteers by means of antibody array analysis. The patients were divided into t...
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| Veröffentlicht in: | Folia biologica 2008-01, Vol.54 (4), p.134-140 |
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| container_title | Folia biologica |
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| creator | Lukesová, S Kopecký, O Vroblová, V Hlávková, D Andrýs, C Morávek, P Cermáková, E |
| description | Using the protein array method we determined the serum levels of a number of angiogenic factors. We identified serum levels of angiogenin, PDGF and MCP-1 (CCL2 chemokine) in serum of 32 patients with RCC, and 14 healthy volunteers by means of antibody array analysis. The patients were divided into three groups according to their disease stages (I+II, III, and IV). We found significant differences between the controls and patients with RCC both pre-operatively and post-operatively in angiogenin, PDGF and MCP-1 serum levels. The increase in angiogenin, PDGF and MCP-1 lasted in patients with RCC stages I-III even without metastases eight weeks post-operatively. The patients with stage IV RCC showed disturbed production of PDGF and MCP-1. Protein array analysis is a powerful tool for the identification of large numbers of trace proteins. Multiplex antibody array is able to provide data more precisely reflecting the nature of pathological processes. |
| doi_str_mv | 10.14712/fb2008054040134 |
| format | Article |
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We identified serum levels of angiogenin, PDGF and MCP-1 (CCL2 chemokine) in serum of 32 patients with RCC, and 14 healthy volunteers by means of antibody array analysis. The patients were divided into three groups according to their disease stages (I+II, III, and IV). We found significant differences between the controls and patients with RCC both pre-operatively and post-operatively in angiogenin, PDGF and MCP-1 serum levels. The increase in angiogenin, PDGF and MCP-1 lasted in patients with RCC stages I-III even without metastases eight weeks post-operatively. The patients with stage IV RCC showed disturbed production of PDGF and MCP-1. Protein array analysis is a powerful tool for the identification of large numbers of trace proteins. Multiplex antibody array is able to provide data more precisely reflecting the nature of pathological processes.</description><identifier>ISSN: 0015-5500</identifier><identifier>EISSN: 2533-7602</identifier><identifier>DOI: 10.14712/fb2008054040134</identifier><identifier>PMID: 18808740</identifier><language>eng</language><publisher>Czech Republic: Charles University in Prague, First Faculty of Medicine</publisher><subject>Aged ; Carcinoma, Renal Cell - blood ; Carcinoma, Renal Cell - pathology ; Carcinoma, Renal Cell - surgery ; Chemokine CCL2 - blood ; Female ; Humans ; Male ; Middle Aged ; Platelet-Derived Growth Factor - metabolism ; Protein Array Analysis ; Ribonuclease, Pancreatic - blood</subject><ispartof>Folia biologica, 2008-01, Vol.54 (4), p.134-140</ispartof><rights>Copyright First Faculty of Medicine 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c277t-4e08721b71330a20549e2a8c257fd0917e894ef2786fdec8a59335112eff1bc63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18808740$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lukesová, S</creatorcontrib><creatorcontrib>Kopecký, O</creatorcontrib><creatorcontrib>Vroblová, V</creatorcontrib><creatorcontrib>Hlávková, D</creatorcontrib><creatorcontrib>Andrýs, C</creatorcontrib><creatorcontrib>Morávek, P</creatorcontrib><creatorcontrib>Cermáková, E</creatorcontrib><title>Determination of angiogenic factors in serum by protein array in patients with renal cell carcinoma</title><title>Folia biologica</title><addtitle>Folia Biol (Praha)</addtitle><description>Using the protein array method we determined the serum levels of a number of angiogenic factors. We identified serum levels of angiogenin, PDGF and MCP-1 (CCL2 chemokine) in serum of 32 patients with RCC, and 14 healthy volunteers by means of antibody array analysis. The patients were divided into three groups according to their disease stages (I+II, III, and IV). We found significant differences between the controls and patients with RCC both pre-operatively and post-operatively in angiogenin, PDGF and MCP-1 serum levels. The increase in angiogenin, PDGF and MCP-1 lasted in patients with RCC stages I-III even without metastases eight weeks post-operatively. The patients with stage IV RCC showed disturbed production of PDGF and MCP-1. Protein array analysis is a powerful tool for the identification of large numbers of trace proteins. 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We identified serum levels of angiogenin, PDGF and MCP-1 (CCL2 chemokine) in serum of 32 patients with RCC, and 14 healthy volunteers by means of antibody array analysis. The patients were divided into three groups according to their disease stages (I+II, III, and IV). We found significant differences between the controls and patients with RCC both pre-operatively and post-operatively in angiogenin, PDGF and MCP-1 serum levels. The increase in angiogenin, PDGF and MCP-1 lasted in patients with RCC stages I-III even without metastases eight weeks post-operatively. The patients with stage IV RCC showed disturbed production of PDGF and MCP-1. Protein array analysis is a powerful tool for the identification of large numbers of trace proteins. Multiplex antibody array is able to provide data more precisely reflecting the nature of pathological processes.</abstract><cop>Czech Republic</cop><pub>Charles University in Prague, First Faculty of Medicine</pub><pmid>18808740</pmid><doi>10.14712/fb2008054040134</doi><tpages>7</tpages></addata></record> |
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| subjects | Aged Carcinoma, Renal Cell - blood Carcinoma, Renal Cell - pathology Carcinoma, Renal Cell - surgery Chemokine CCL2 - blood Female Humans Male Middle Aged Platelet-Derived Growth Factor - metabolism Protein Array Analysis Ribonuclease, Pancreatic - blood |
| title | Determination of angiogenic factors in serum by protein array in patients with renal cell carcinoma |
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