Down‐regulation and redistribution of GPV/GPVf2, a subunit of von Willebrand factor receptor (GPIb/IX/V complex), on the surface membrane of thrombin‐stimulated human platelets
We have studied down‐regulation and redistribution of glycoprotein V (GPV) and its fragment GPVf2, a subunit of a receptor for von Willebrand factor (VWF), on the surface membrane of thrombin and thrombin receptor activating peptide (TRAP) stimulated platelets by using a newly developed GPVf2‐specif...
Gespeichert in:
| Veröffentlicht in: | British journal of haematology 1999-01, Vol.104 (1), p.55-63 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 63 |
|---|---|
| container_issue | 1 |
| container_start_page | 55 |
| container_title | British journal of haematology |
| container_volume | 104 |
| creator | Kawano, Hironori Suzuki, Hidenori Tanoue, Kenjiro Kimura, Akiro Fujimura, Kingo |
| description | We have studied down‐regulation and redistribution of glycoprotein V (GPV) and its fragment GPVf2, a subunit of a receptor for von Willebrand factor (VWF), on the surface membrane of thrombin and thrombin receptor activating peptide (TRAP) stimulated platelets by using a newly developed GPVf2‐specific monoclonal antibody (1D9). Immunoelectronmicroscopical studies revealed that about 50% each of total GPV and GPIX were expressed on the surface membrane of the resting human platelets, and about 83% of GPlbα was expressed on the surface. In thrombin‐stimulated platelets, the surface GPIbα, GPIX and GPV, after hydrolysis by thrombin, was converted to GPVf2, translocated from the surface to the intraplatelet pool and then again redistributed to the surface. In TRAP‐stimulated platelets, GPIbα, GPIX and GPV, without conversion to GPVf2, were translocated from the surface to the intraplatelet pool and then returned to the surface. Ristocetin‐induced agglutinations of both the thrombin‐ and TRAP‐stimulated platelets were lowered during the decreased surface expressions of GPIbα, GPIX and GPV/GPVf2 and then normalized when these GPs were again redistributed onto the surface, indicating that the redistributed GPIb/IX/Vf2 complex on the surface can act as a VWF receptor as efficiently as an intact GPIb/IX/V. |
| doi_str_mv | 10.1046/j.1365-2141.1999.01131.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_69580381</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>38480724</sourcerecordid><originalsourceid>FETCH-LOGICAL-p2821-e601948e1d87046dbe9e9b5080bf96ce8e2c2891880a9eee0c2d46b81686e70b3</originalsourceid><addsrcrecordid>eNpdks1u1DAQxyMEokvhFZCFECpSk_Uku4594EALbBdVogco3Cw7mbBeOR_YCd3eeAQehifiSbDb5UMcLI9nfv_RaPxPEgI0A7pg820GBVumOSwgAyFERgEKyHZ3ktmfwt1kRikt0yDgB8kD77eUQkGXcD85AErzsoR8lvx41V91P799d_h5smo0fUdUVxOHtfGjM3q6SfUNWV1czsNp8mOiiJ_01Jkx5r-G8kdjLWoXhY2qxt4FfYVDDI5WF2s9X3-aX5KqbweLu-fHJEjGDYYuLuBIWmyjGGO7ceP6Vps4kh9NG2fCmmymVnVkiA-Lo3-Y3GuU9fhofx8mH968fn96lp6_W61PX56nQ85zSJFREAuOUPMybK3WKFDoJeVUN4JVyDGvci6Ac6oEItIqrxdMc2CcYUl1cZg8u-07uP7LhH6UrfEVWhuG7ScvmVhyWnAI4JP_wG0_uS7MJkFwBnlJI_R4D026xVoOzrTKXcvfnxGAp3tA-UrZJuykMv4vxwpeLkXAXtxiV8bi9T9tZPSG3MpoARktIKM35I035E6evD2LUfELxsGvdg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>198612701</pqid></control><display><type>article</type><title>Down‐regulation and redistribution of GPV/GPVf2, a subunit of von Willebrand factor receptor (GPIb/IX/V complex), on the surface membrane of thrombin‐stimulated human platelets</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Wiley Online Library Free Content</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Kawano, Hironori ; Suzuki, Hidenori ; Tanoue, Kenjiro ; Kimura, Akiro ; Fujimura, Kingo</creator><creatorcontrib>Kawano, Hironori ; Suzuki, Hidenori ; Tanoue, Kenjiro ; Kimura, Akiro ; Fujimura, Kingo</creatorcontrib><description>We have studied down‐regulation and redistribution of glycoprotein V (GPV) and its fragment GPVf2, a subunit of a receptor for von Willebrand factor (VWF), on the surface membrane of thrombin and thrombin receptor activating peptide (TRAP) stimulated platelets by using a newly developed GPVf2‐specific monoclonal antibody (1D9). Immunoelectronmicroscopical studies revealed that about 50% each of total GPV and GPIX were expressed on the surface membrane of the resting human platelets, and about 83% of GPlbα was expressed on the surface. In thrombin‐stimulated platelets, the surface GPIbα, GPIX and GPV, after hydrolysis by thrombin, was converted to GPVf2, translocated from the surface to the intraplatelet pool and then again redistributed to the surface. In TRAP‐stimulated platelets, GPIbα, GPIX and GPV, without conversion to GPVf2, were translocated from the surface to the intraplatelet pool and then returned to the surface. Ristocetin‐induced agglutinations of both the thrombin‐ and TRAP‐stimulated platelets were lowered during the decreased surface expressions of GPIbα, GPIX and GPV/GPVf2 and then normalized when these GPs were again redistributed onto the surface, indicating that the redistributed GPIb/IX/Vf2 complex on the surface can act as a VWF receptor as efficiently as an intact GPIb/IX/V.</description><identifier>ISSN: 0007-1048</identifier><identifier>EISSN: 1365-2141</identifier><identifier>DOI: 10.1046/j.1365-2141.1999.01131.x</identifier><identifier>PMID: 10027712</identifier><identifier>CODEN: BJHEAL</identifier><language>eng</language><publisher>Oxford, U.K. and Cambridge, USA: Blackwell Science Ltd</publisher><subject>Biological and medical sciences ; Blood coagulation. Blood cells ; Blood Platelets - metabolism ; Down-Regulation ; Flow Cytometry ; Fundamental and applied biological sciences. Psychology ; GPIb/IX/V complex ; GPV, GPVf2 ; Hematology ; Humans ; immunoelectronmicroscopy ; Immunohistochemistry ; Microscopy, Electron - methods ; Molecular and cellular biology ; Peptide Fragments - metabolism ; Platelet ; Platelet Glycoprotein GPIb-IX Complex - metabolism ; Platelet Membrane Glycoproteins - metabolism ; Receptors, Cell Surface - metabolism ; Thrombin - pharmacology ; von Willebrand factor receptor</subject><ispartof>British journal of haematology, 1999-01, Vol.104 (1), p.55-63</ispartof><rights>1999 INIST-CNRS</rights><rights>Copyright Blackwell Scientific Publications Ltd. Jan 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2141.1999.01131.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2141.1999.01131.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,4010,27900,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1638759$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10027712$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kawano, Hironori</creatorcontrib><creatorcontrib>Suzuki, Hidenori</creatorcontrib><creatorcontrib>Tanoue, Kenjiro</creatorcontrib><creatorcontrib>Kimura, Akiro</creatorcontrib><creatorcontrib>Fujimura, Kingo</creatorcontrib><title>Down‐regulation and redistribution of GPV/GPVf2, a subunit of von Willebrand factor receptor (GPIb/IX/V complex), on the surface membrane of thrombin‐stimulated human platelets</title><title>British journal of haematology</title><addtitle>Br J Haematol</addtitle><description>We have studied down‐regulation and redistribution of glycoprotein V (GPV) and its fragment GPVf2, a subunit of a receptor for von Willebrand factor (VWF), on the surface membrane of thrombin and thrombin receptor activating peptide (TRAP) stimulated platelets by using a newly developed GPVf2‐specific monoclonal antibody (1D9). Immunoelectronmicroscopical studies revealed that about 50% each of total GPV and GPIX were expressed on the surface membrane of the resting human platelets, and about 83% of GPlbα was expressed on the surface. In thrombin‐stimulated platelets, the surface GPIbα, GPIX and GPV, after hydrolysis by thrombin, was converted to GPVf2, translocated from the surface to the intraplatelet pool and then again redistributed to the surface. In TRAP‐stimulated platelets, GPIbα, GPIX and GPV, without conversion to GPVf2, were translocated from the surface to the intraplatelet pool and then returned to the surface. Ristocetin‐induced agglutinations of both the thrombin‐ and TRAP‐stimulated platelets were lowered during the decreased surface expressions of GPIbα, GPIX and GPV/GPVf2 and then normalized when these GPs were again redistributed onto the surface, indicating that the redistributed GPIb/IX/Vf2 complex on the surface can act as a VWF receptor as efficiently as an intact GPIb/IX/V.</description><subject>Biological and medical sciences</subject><subject>Blood coagulation. Blood cells</subject><subject>Blood Platelets - metabolism</subject><subject>Down-Regulation</subject><subject>Flow Cytometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GPIb/IX/V complex</subject><subject>GPV, GPVf2</subject><subject>Hematology</subject><subject>Humans</subject><subject>immunoelectronmicroscopy</subject><subject>Immunohistochemistry</subject><subject>Microscopy, Electron - methods</subject><subject>Molecular and cellular biology</subject><subject>Peptide Fragments - metabolism</subject><subject>Platelet</subject><subject>Platelet Glycoprotein GPIb-IX Complex - metabolism</subject><subject>Platelet Membrane Glycoproteins - metabolism</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>Thrombin - pharmacology</subject><subject>von Willebrand factor receptor</subject><issn>0007-1048</issn><issn>1365-2141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdks1u1DAQxyMEokvhFZCFECpSk_Uku4594EALbBdVogco3Cw7mbBeOR_YCd3eeAQehifiSbDb5UMcLI9nfv_RaPxPEgI0A7pg820GBVumOSwgAyFERgEKyHZ3ktmfwt1kRikt0yDgB8kD77eUQkGXcD85AErzsoR8lvx41V91P799d_h5smo0fUdUVxOHtfGjM3q6SfUNWV1czsNp8mOiiJ_01Jkx5r-G8kdjLWoXhY2qxt4FfYVDDI5WF2s9X3-aX5KqbweLu-fHJEjGDYYuLuBIWmyjGGO7ceP6Vps4kh9NG2fCmmymVnVkiA-Lo3-Y3GuU9fhofx8mH968fn96lp6_W61PX56nQ85zSJFREAuOUPMybK3WKFDoJeVUN4JVyDGvci6Ac6oEItIqrxdMc2CcYUl1cZg8u-07uP7LhH6UrfEVWhuG7ScvmVhyWnAI4JP_wG0_uS7MJkFwBnlJI_R4D026xVoOzrTKXcvfnxGAp3tA-UrZJuykMv4vxwpeLkXAXtxiV8bi9T9tZPSG3MpoARktIKM35I035E6evD2LUfELxsGvdg</recordid><startdate>199901</startdate><enddate>199901</enddate><creator>Kawano, Hironori</creator><creator>Suzuki, Hidenori</creator><creator>Tanoue, Kenjiro</creator><creator>Kimura, Akiro</creator><creator>Fujimura, Kingo</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><general>Blackwell Publishing Ltd</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>199901</creationdate><title>Down‐regulation and redistribution of GPV/GPVf2, a subunit of von Willebrand factor receptor (GPIb/IX/V complex), on the surface membrane of thrombin‐stimulated human platelets</title><author>Kawano, Hironori ; Suzuki, Hidenori ; Tanoue, Kenjiro ; Kimura, Akiro ; Fujimura, Kingo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p2821-e601948e1d87046dbe9e9b5080bf96ce8e2c2891880a9eee0c2d46b81686e70b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Biological and medical sciences</topic><topic>Blood coagulation. Blood cells</topic><topic>Blood Platelets - metabolism</topic><topic>Down-Regulation</topic><topic>Flow Cytometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GPIb/IX/V complex</topic><topic>GPV, GPVf2</topic><topic>Hematology</topic><topic>Humans</topic><topic>immunoelectronmicroscopy</topic><topic>Immunohistochemistry</topic><topic>Microscopy, Electron - methods</topic><topic>Molecular and cellular biology</topic><topic>Peptide Fragments - metabolism</topic><topic>Platelet</topic><topic>Platelet Glycoprotein GPIb-IX Complex - metabolism</topic><topic>Platelet Membrane Glycoproteins - metabolism</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>Thrombin - pharmacology</topic><topic>von Willebrand factor receptor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kawano, Hironori</creatorcontrib><creatorcontrib>Suzuki, Hidenori</creatorcontrib><creatorcontrib>Tanoue, Kenjiro</creatorcontrib><creatorcontrib>Kimura, Akiro</creatorcontrib><creatorcontrib>Fujimura, Kingo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>British journal of haematology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kawano, Hironori</au><au>Suzuki, Hidenori</au><au>Tanoue, Kenjiro</au><au>Kimura, Akiro</au><au>Fujimura, Kingo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Down‐regulation and redistribution of GPV/GPVf2, a subunit of von Willebrand factor receptor (GPIb/IX/V complex), on the surface membrane of thrombin‐stimulated human platelets</atitle><jtitle>British journal of haematology</jtitle><addtitle>Br J Haematol</addtitle><date>1999-01</date><risdate>1999</risdate><volume>104</volume><issue>1</issue><spage>55</spage><epage>63</epage><pages>55-63</pages><issn>0007-1048</issn><eissn>1365-2141</eissn><coden>BJHEAL</coden><abstract>We have studied down‐regulation and redistribution of glycoprotein V (GPV) and its fragment GPVf2, a subunit of a receptor for von Willebrand factor (VWF), on the surface membrane of thrombin and thrombin receptor activating peptide (TRAP) stimulated platelets by using a newly developed GPVf2‐specific monoclonal antibody (1D9). Immunoelectronmicroscopical studies revealed that about 50% each of total GPV and GPIX were expressed on the surface membrane of the resting human platelets, and about 83% of GPlbα was expressed on the surface. In thrombin‐stimulated platelets, the surface GPIbα, GPIX and GPV, after hydrolysis by thrombin, was converted to GPVf2, translocated from the surface to the intraplatelet pool and then again redistributed to the surface. In TRAP‐stimulated platelets, GPIbα, GPIX and GPV, without conversion to GPVf2, were translocated from the surface to the intraplatelet pool and then returned to the surface. Ristocetin‐induced agglutinations of both the thrombin‐ and TRAP‐stimulated platelets were lowered during the decreased surface expressions of GPIbα, GPIX and GPV/GPVf2 and then normalized when these GPs were again redistributed onto the surface, indicating that the redistributed GPIb/IX/Vf2 complex on the surface can act as a VWF receptor as efficiently as an intact GPIb/IX/V.</abstract><cop>Oxford, U.K. and Cambridge, USA</cop><pub>Blackwell Science Ltd</pub><pmid>10027712</pmid><doi>10.1046/j.1365-2141.1999.01131.x</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0007-1048 |
| ispartof | British journal of haematology, 1999-01, Vol.104 (1), p.55-63 |
| issn | 0007-1048 1365-2141 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69580381 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Wiley Online Library Free Content; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Biological and medical sciences Blood coagulation. Blood cells Blood Platelets - metabolism Down-Regulation Flow Cytometry Fundamental and applied biological sciences. Psychology GPIb/IX/V complex GPV, GPVf2 Hematology Humans immunoelectronmicroscopy Immunohistochemistry Microscopy, Electron - methods Molecular and cellular biology Peptide Fragments - metabolism Platelet Platelet Glycoprotein GPIb-IX Complex - metabolism Platelet Membrane Glycoproteins - metabolism Receptors, Cell Surface - metabolism Thrombin - pharmacology von Willebrand factor receptor |
| title | Down‐regulation and redistribution of GPV/GPVf2, a subunit of von Willebrand factor receptor (GPIb/IX/V complex), on the surface membrane of thrombin‐stimulated human platelets |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T17%3A54%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Down%E2%80%90regulation%20and%20redistribution%20of%20GPV/GPVf2,%20a%20subunit%20of%20von%20Willebrand%20factor%20receptor%20(GPIb/IX/V%20complex),%20on%20the%20surface%20membrane%20of%20thrombin%E2%80%90stimulated%20human%20platelets&rft.jtitle=British%20journal%20of%20haematology&rft.au=Kawano,%20Hironori&rft.date=1999-01&rft.volume=104&rft.issue=1&rft.spage=55&rft.epage=63&rft.pages=55-63&rft.issn=0007-1048&rft.eissn=1365-2141&rft.coden=BJHEAL&rft_id=info:doi/10.1046/j.1365-2141.1999.01131.x&rft_dat=%3Cproquest_pubme%3E38480724%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=198612701&rft_id=info:pmid/10027712&rfr_iscdi=true |