High-level and effective production of human mannan-binding lectin (MBL) in Chinese hamster ovary (CHO) cells

We have developed a high-expression system of recombinant human mannan-binding lectin (MBL) with CHO cells. Geneticin-resistant transformants harboring human MBL cDNA in the expression vector pNOW/CMV-A were screened by immunoblot analysis for secretion of recombinant MBL. Cloning and selection by b...

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Veröffentlicht in:	Journal of immunological methods 1999, Vol.222 (1), p.135-144
Hauptverfasser:	Ohtani, Katsuki, Suzuki, Yasuhiko, Eda, Souji, Kawai, Takao, Kase, Tetsuo, Keshi, Hiroyuki, Sakai, Yoshinori, Yamamoto, Satoshi, Sakamoto, Takashi, Wakamiya, Nobutaka
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Anti-viral activity Biological and medical sciences Biotechnology Carrier Proteins - biosynthesis Carrier Proteins - genetics Carrier Proteins - isolation & purification CHO Cells - metabolism CHO expression system Collectins Complement activation Cricetinae DNA, Complementary - genetics DNA, Complementary - metabolism Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Gene Amplification Hemagglutination Inhibition Tests Humans Mannan-binding lectin (MBL) Methods. Procedures. Technologies Protein engineering Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Recombinant Proteins - isolation & purification Transformation, Genetic
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