Dexamethasone modulation of multidrug transporters in normal tissues
The expression of P-glycoprotein (P-gp) and canalicular multispecific organic anion transporter (cMOAT or Mrp2) was evaluated by Western blotting analysis of rat tissues isolated following daily administration (1 mg kg −1 day −1) of dexamethasone over 4 days. Dexamethasone rapidly increased P-gp exp...
Gespeichert in:
| Veröffentlicht in: | FEBS letters 1999-01, Vol.442 (2), p.208-214 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 214 |
|---|---|
| container_issue | 2 |
| container_start_page | 208 |
| container_title | FEBS letters |
| container_volume | 442 |
| creator | Demeule, Michel Jodoin, Julie Beaulieu, Édith Brossard, Mathieu Béliveau, Richard |
| description | The expression of P-glycoprotein (P-gp) and canalicular multispecific organic anion transporter (cMOAT or Mrp2) was evaluated by Western blotting analysis of rat tissues isolated following daily administration (1 mg kg
−1 day
−1) of dexamethasone over 4 days. Dexamethasone rapidly increased P-gp expression more than 4.5- and 2-fold in liver and lung, respectively, while it was decreased 40% in kidney. cMOAT expression was increased 2-fold in liver and kidney following dexamethasone treatment. The levels of both proteins returned to control values by 6 days after the conclusion of dexamethasone administration. These results indicate that dexamethasone can modulate P-gp and cMOAT expression in specific rat tissues and may have significant relevance for patients treated with dexamethasone as a single agent or in combination therapy with other drugs. |
| doi_str_mv | 10.1016/S0014-5793(98)01663-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69577087</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0014579398016639</els_id><sourcerecordid>69577087</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4919-72ecccd83630f8c947954046ad8d145f52b1d544dc7eaa4f762029460c585bd83</originalsourceid><addsrcrecordid>eNqNkF9PwyAUxYnRzDn9CEv6ZPShCi208GR0f9RkiQ_qM2Fwq5i2TGjVfXvbbdmrPhHuuecc-CE0JviKYJJdP2NMaMxykV4IftlNsjQWB2hIeJ7GKc34IRruV47RSQgfuLtzIgZoIEQiME6HaDqFH1VB866CqyGqnGlL1VhXR66IqrZsrPHtW9R4VYeV8w34ENk6qp2vVBk1NoQWwik6KlQZ4Gx3jtDrfPYyeYgXT_ePk9tFrKkgIs4T0FobnmYpLrgWNBeMYpopww2hrGDJkhhGqdE5KEWLPEtwImiGNeNs2flG6Hybu_Lus-ttZGWDhrJUNbg2yEywPMfd_0eIbRe1dyF4KOTK20r5tSRY9vTkhp7s0UjB5YaeFJ1vvCtolxWYvWuHq9Mftvq3LWH9v1A5n90lG6UXBN-M-6qbbRR0wL4seBm0hVqDsR50I42zfzz2F4bfk10</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69577087</pqid></control><display><type>article</type><title>Dexamethasone modulation of multidrug transporters in normal tissues</title><source>Wiley Online Library - AutoHoldings Journals</source><source>MEDLINE</source><source>Elsevier ScienceDirect Journals Complete</source><source>Wiley Online Library Free Content</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Demeule, Michel ; Jodoin, Julie ; Beaulieu, Édith ; Brossard, Mathieu ; Béliveau, Richard</creator><creatorcontrib>Demeule, Michel ; Jodoin, Julie ; Beaulieu, Édith ; Brossard, Mathieu ; Béliveau, Richard</creatorcontrib><description>The expression of P-glycoprotein (P-gp) and canalicular multispecific organic anion transporter (cMOAT or Mrp2) was evaluated by Western blotting analysis of rat tissues isolated following daily administration (1 mg kg
−1 day
−1) of dexamethasone over 4 days. Dexamethasone rapidly increased P-gp expression more than 4.5- and 2-fold in liver and lung, respectively, while it was decreased 40% in kidney. cMOAT expression was increased 2-fold in liver and kidney following dexamethasone treatment. The levels of both proteins returned to control values by 6 days after the conclusion of dexamethasone administration. These results indicate that dexamethasone can modulate P-gp and cMOAT expression in specific rat tissues and may have significant relevance for patients treated with dexamethasone as a single agent or in combination therapy with other drugs.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/S0014-5793(98)01663-9</identifier><identifier>PMID: 9929003</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Animals ; Anion Transport Proteins ; ATP Binding Cassette Transporter, Subfamily B, Member 1 - immunology ; ATP Binding Cassette Transporter, Subfamily B, Member 1 - metabolism ; BBM, brush border membranes ; Blotting, Western ; Carrier Proteins - immunology ; Carrier Proteins - metabolism ; cMOAT ; Dexamethasone ; Dexamethasone - administration & dosage ; Dexamethasone - pharmacology ; Drug Resistance, Multiple ; ECL, enhanced chemiluminescence ; Gene Expression - drug effects ; IAAP, [125I]iodoaryl azidoprazosin ; Kidney - drug effects ; Kidney - enzymology ; Kidney - metabolism ; Liver - drug effects ; Liver - enzymology ; Liver - metabolism ; Lung - drug effects ; Lung - enzymology ; Lung - metabolism ; mAb, monoclonal antibody ; Male ; Membranes - enzymology ; Molecular Weight ; Mrp2 ; P-glycoprotein ; P-gp, P-glycoprotein ; pAb, polyclonal antibody ; Protein expression ; Protein Isoforms - immunology ; Protein Isoforms - metabolism ; PVDF, polyvinylidene difluoride ; Rats ; Rats, Sprague-Dawley ; spgp, sister P-glycoprotein ; TBS-T, Tris buffered saline with 0.3% Tween 20</subject><ispartof>FEBS letters, 1999-01, Vol.442 (2), p.208-214</ispartof><rights>1999 Federation of European Biochemical Societies</rights><rights>FEBS Letters 442 (1999) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4919-72ecccd83630f8c947954046ad8d145f52b1d544dc7eaa4f762029460c585bd83</citedby><cites>FETCH-LOGICAL-c4919-72ecccd83630f8c947954046ad8d145f52b1d544dc7eaa4f762029460c585bd83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0014-5793%2898%2901663-9$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0014-5793(98)01663-9$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1417,1433,3550,27924,27925,45574,45575,45995,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9929003$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Demeule, Michel</creatorcontrib><creatorcontrib>Jodoin, Julie</creatorcontrib><creatorcontrib>Beaulieu, Édith</creatorcontrib><creatorcontrib>Brossard, Mathieu</creatorcontrib><creatorcontrib>Béliveau, Richard</creatorcontrib><title>Dexamethasone modulation of multidrug transporters in normal tissues</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>The expression of P-glycoprotein (P-gp) and canalicular multispecific organic anion transporter (cMOAT or Mrp2) was evaluated by Western blotting analysis of rat tissues isolated following daily administration (1 mg kg
−1 day
−1) of dexamethasone over 4 days. Dexamethasone rapidly increased P-gp expression more than 4.5- and 2-fold in liver and lung, respectively, while it was decreased 40% in kidney. cMOAT expression was increased 2-fold in liver and kidney following dexamethasone treatment. The levels of both proteins returned to control values by 6 days after the conclusion of dexamethasone administration. These results indicate that dexamethasone can modulate P-gp and cMOAT expression in specific rat tissues and may have significant relevance for patients treated with dexamethasone as a single agent or in combination therapy with other drugs.</description><subject>Animals</subject><subject>Anion Transport Proteins</subject><subject>ATP Binding Cassette Transporter, Subfamily B, Member 1 - immunology</subject><subject>ATP Binding Cassette Transporter, Subfamily B, Member 1 - metabolism</subject><subject>BBM, brush border membranes</subject><subject>Blotting, Western</subject><subject>Carrier Proteins - immunology</subject><subject>Carrier Proteins - metabolism</subject><subject>cMOAT</subject><subject>Dexamethasone</subject><subject>Dexamethasone - administration & dosage</subject><subject>Dexamethasone - pharmacology</subject><subject>Drug Resistance, Multiple</subject><subject>ECL, enhanced chemiluminescence</subject><subject>Gene Expression - drug effects</subject><subject>IAAP, [125I]iodoaryl azidoprazosin</subject><subject>Kidney - drug effects</subject><subject>Kidney - enzymology</subject><subject>Kidney - metabolism</subject><subject>Liver - drug effects</subject><subject>Liver - enzymology</subject><subject>Liver - metabolism</subject><subject>Lung - drug effects</subject><subject>Lung - enzymology</subject><subject>Lung - metabolism</subject><subject>mAb, monoclonal antibody</subject><subject>Male</subject><subject>Membranes - enzymology</subject><subject>Molecular Weight</subject><subject>Mrp2</subject><subject>P-glycoprotein</subject><subject>P-gp, P-glycoprotein</subject><subject>pAb, polyclonal antibody</subject><subject>Protein expression</subject><subject>Protein Isoforms - immunology</subject><subject>Protein Isoforms - metabolism</subject><subject>PVDF, polyvinylidene difluoride</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>spgp, sister P-glycoprotein</subject><subject>TBS-T, Tris buffered saline with 0.3% Tween 20</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkF9PwyAUxYnRzDn9CEv6ZPShCi208GR0f9RkiQ_qM2Fwq5i2TGjVfXvbbdmrPhHuuecc-CE0JviKYJJdP2NMaMxykV4IftlNsjQWB2hIeJ7GKc34IRruV47RSQgfuLtzIgZoIEQiME6HaDqFH1VB866CqyGqnGlL1VhXR66IqrZsrPHtW9R4VYeV8w34ENk6qp2vVBk1NoQWwik6KlQZ4Gx3jtDrfPYyeYgXT_ePk9tFrKkgIs4T0FobnmYpLrgWNBeMYpopww2hrGDJkhhGqdE5KEWLPEtwImiGNeNs2flG6Hybu_Lus-ttZGWDhrJUNbg2yEywPMfd_0eIbRe1dyF4KOTK20r5tSRY9vTkhp7s0UjB5YaeFJ1vvCtolxWYvWuHq9Mftvq3LWH9v1A5n90lG6UXBN-M-6qbbRR0wL4seBm0hVqDsR50I42zfzz2F4bfk10</recordid><startdate>19990115</startdate><enddate>19990115</enddate><creator>Demeule, Michel</creator><creator>Jodoin, Julie</creator><creator>Beaulieu, Édith</creator><creator>Brossard, Mathieu</creator><creator>Béliveau, Richard</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19990115</creationdate><title>Dexamethasone modulation of multidrug transporters in normal tissues</title><author>Demeule, Michel ; Jodoin, Julie ; Beaulieu, Édith ; Brossard, Mathieu ; Béliveau, Richard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4919-72ecccd83630f8c947954046ad8d145f52b1d544dc7eaa4f762029460c585bd83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Animals</topic><topic>Anion Transport Proteins</topic><topic>ATP Binding Cassette Transporter, Subfamily B, Member 1 - immunology</topic><topic>ATP Binding Cassette Transporter, Subfamily B, Member 1 - metabolism</topic><topic>BBM, brush border membranes</topic><topic>Blotting, Western</topic><topic>Carrier Proteins - immunology</topic><topic>Carrier Proteins - metabolism</topic><topic>cMOAT</topic><topic>Dexamethasone</topic><topic>Dexamethasone - administration & dosage</topic><topic>Dexamethasone - pharmacology</topic><topic>Drug Resistance, Multiple</topic><topic>ECL, enhanced chemiluminescence</topic><topic>Gene Expression - drug effects</topic><topic>IAAP, [125I]iodoaryl azidoprazosin</topic><topic>Kidney - drug effects</topic><topic>Kidney - enzymology</topic><topic>Kidney - metabolism</topic><topic>Liver - drug effects</topic><topic>Liver - enzymology</topic><topic>Liver - metabolism</topic><topic>Lung - drug effects</topic><topic>Lung - enzymology</topic><topic>Lung - metabolism</topic><topic>mAb, monoclonal antibody</topic><topic>Male</topic><topic>Membranes - enzymology</topic><topic>Molecular Weight</topic><topic>Mrp2</topic><topic>P-glycoprotein</topic><topic>P-gp, P-glycoprotein</topic><topic>pAb, polyclonal antibody</topic><topic>Protein expression</topic><topic>Protein Isoforms - immunology</topic><topic>Protein Isoforms - metabolism</topic><topic>PVDF, polyvinylidene difluoride</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>spgp, sister P-glycoprotein</topic><topic>TBS-T, Tris buffered saline with 0.3% Tween 20</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Demeule, Michel</creatorcontrib><creatorcontrib>Jodoin, Julie</creatorcontrib><creatorcontrib>Beaulieu, Édith</creatorcontrib><creatorcontrib>Brossard, Mathieu</creatorcontrib><creatorcontrib>Béliveau, Richard</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Demeule, Michel</au><au>Jodoin, Julie</au><au>Beaulieu, Édith</au><au>Brossard, Mathieu</au><au>Béliveau, Richard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dexamethasone modulation of multidrug transporters in normal tissues</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1999-01-15</date><risdate>1999</risdate><volume>442</volume><issue>2</issue><spage>208</spage><epage>214</epage><pages>208-214</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>The expression of P-glycoprotein (P-gp) and canalicular multispecific organic anion transporter (cMOAT or Mrp2) was evaluated by Western blotting analysis of rat tissues isolated following daily administration (1 mg kg
−1 day
−1) of dexamethasone over 4 days. Dexamethasone rapidly increased P-gp expression more than 4.5- and 2-fold in liver and lung, respectively, while it was decreased 40% in kidney. cMOAT expression was increased 2-fold in liver and kidney following dexamethasone treatment. The levels of both proteins returned to control values by 6 days after the conclusion of dexamethasone administration. These results indicate that dexamethasone can modulate P-gp and cMOAT expression in specific rat tissues and may have significant relevance for patients treated with dexamethasone as a single agent or in combination therapy with other drugs.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>9929003</pmid><doi>10.1016/S0014-5793(98)01663-9</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1999-01, Vol.442 (2), p.208-214 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_69577087 |
| source | Wiley Online Library - AutoHoldings Journals; MEDLINE; Elsevier ScienceDirect Journals Complete; Wiley Online Library Free Content; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Anion Transport Proteins ATP Binding Cassette Transporter, Subfamily B, Member 1 - immunology ATP Binding Cassette Transporter, Subfamily B, Member 1 - metabolism BBM, brush border membranes Blotting, Western Carrier Proteins - immunology Carrier Proteins - metabolism cMOAT Dexamethasone Dexamethasone - administration & dosage Dexamethasone - pharmacology Drug Resistance, Multiple ECL, enhanced chemiluminescence Gene Expression - drug effects IAAP, [125I]iodoaryl azidoprazosin Kidney - drug effects Kidney - enzymology Kidney - metabolism Liver - drug effects Liver - enzymology Liver - metabolism Lung - drug effects Lung - enzymology Lung - metabolism mAb, monoclonal antibody Male Membranes - enzymology Molecular Weight Mrp2 P-glycoprotein P-gp, P-glycoprotein pAb, polyclonal antibody Protein expression Protein Isoforms - immunology Protein Isoforms - metabolism PVDF, polyvinylidene difluoride Rats Rats, Sprague-Dawley spgp, sister P-glycoprotein TBS-T, Tris buffered saline with 0.3% Tween 20 |
| title | Dexamethasone modulation of multidrug transporters in normal tissues |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T10%3A01%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Dexamethasone%20modulation%20of%20multidrug%20transporters%20in%20normal%20tissues&rft.jtitle=FEBS%20letters&rft.au=Demeule,%20Michel&rft.date=1999-01-15&rft.volume=442&rft.issue=2&rft.spage=208&rft.epage=214&rft.pages=208-214&rft.issn=0014-5793&rft.eissn=1873-3468&rft_id=info:doi/10.1016/S0014-5793(98)01663-9&rft_dat=%3Cproquest_cross%3E69577087%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=69577087&rft_id=info:pmid/9929003&rft_els_id=S0014579398016639&rfr_iscdi=true |