Development and standardization of methods to evaluate the antibody response to an HIV-1 candidate vaccine in secretions and sera of seronegative vaccine recipients

Enzyme-linked immunosorbent assays (ELISA) were developed to test, in serum and mucosal samples, total IgG, total IgA, serum albumin, and anti-gp120 MN and anti-p24 LAI IgG and IgA levels. These ELISAs were optimized according to reagents and experimental conditions. Inter- and intra-assay coefficie...

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Veröffentlicht in:	Journal of immunological methods 1999, Vol.222 (1), p.111-124
Hauptverfasser:	Raux, M, Finkielsztejn, L, Salmon-Céron, D, Bouchez, H, Excler, J.L, Dulioust, E, Grouin, J.M, Sicard, D, Blondeau, C
Format:	Artikel
Sprache:	eng
Schlagworte:	AIDS Vaccines - immunology AIDS Vaccines - pharmacology AIDS/HIV Biological and medical sciences Body Fluids - chemistry Body Fluids - immunology ELISA Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology Gene Products, gag - immunology gp120 MN HIV Antibodies - analysis HIV Antibodies - biosynthesis HIV Antibodies - blood HIV Envelope Protein gp120 - immunology HIV Seronegativity - immunology HIV-1 HIV-1 - immunology Human immunodeficiency virus 1 Humans IgA IgG Immunoglobulin A - analysis Immunoglobulin A - biosynthesis Immunoglobulin A - blood Immunoglobulin G - analysis Immunoglobulin G - biosynthesis Immunoglobulin G - blood Linear Models Microbiology Mucosal immunity p24 LAI Reference Standards Reproducibility of Results Saliva - chemistry Saliva - immunology Sensitivity and Specificity Serum Albumin - analysis Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Virology
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